1. Use the prokaryotic gene DNA sequence below to answer the following questions: 1 11 21 31 ATGAAGCTAC 51 TTTGCCCAGG 101 TTTGACAGTC 61 AGGTTTAACA 111 TTCTATCGAA CAAGCATATA ATCAGCGATA 71 ACATGTCAAG 121 d. Where is the 3' UTR? Circle one. 12-72 35-72 -35 consensus sequence: -10 consensus sequence (Pribnow box): Shine-Delgarno sequence in the corresponding mRNA: Start (initiation) codon in the corresponding mRNA: Stop (termination) codon in the corresponding mRNA: 81 131 TGCTCCAAAG AAAAACCGAA ACATCTGACA GGGAAAAAAT CCCCCCCCCC TTTTTTTTTTTTTTTTCCCG 109-146 41 a. Write the corresponding sequences, circle & label them in the sequence above: (label as -35) (label as PB) 91 c. What will be the nascent polypeptide sequence translated from this mRNA? 141 (label as SD) (label as start) b. What region of this prokaryotic DNA sequence will be transcribed into mRNA? Circle one. 1-120 35-108 54-146 72-146 (label as stop) 121-146

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Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.