1. Imagine you are a student in Alfred Hershey and Martha Chase's lab in the late 1940s. You are given five test tubes containing E. coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forgot to mark the tubes and are uncertain about which were labeled with ³2P and which with 35S. You place the contents of each tube in a blender and turn it on for a few seconds to shear off the phage protein coats. You then centrifuge the contents to separate the protein coats and the cells. You check for the presence of radioactivity and obtain the following results. Which tubes contained E. coli infected with ³2P- labeled phage? Explain your answer. Tube number 1 2 3 4 5 Radioactivity present in Cells Protein coats Protein coats Cells Cells

KEY: Hydrophobic interactions: Salt bridge: Covalent bonds: Hydrogen bonding: Metal ion coordination: 9. 10What components of the plasma membrane might you drug interact with? Explain can use as many components as you need (may need more or less). Component 1 and why: Component 2 and why: Component 3 and why:

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