1. Flip-Flop is considered the LEAST likely to happen of the four membrane movements because it is the LEAST energetically favorable movement of a single fatty acid. For this question DRAW the action of a one phopholipid being flipped from outside facing plasma membrane, to the inside facing plasma membrane. On your drawing put an X near areas of the phospholipids that do NOT want to interact during this process.

4. The graph below represents two different modes of transport across a biological membrane. One employs a membrane protein and the other does not. Which line represents transport with a membrane protein? Label each line with the mode of transport. There are three key characteristics of transport across a biological membrane using a membrane protein. What are these three key features? List and describe each feature. 107 A B Concentration difference across the mebrane Rate of Transport

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Where is the KV1.1 (a membrane protein) polypeptide synthesized in the cell?  (word bank: cytoplasm, nucleus, rough ER, smooth ER, mitochondria)    The amino acids that line the outside of the KV1.1 protein (i.e., those in contact with the phospholipid bilayer's fatty acids tails) are more likely to be _______(word bank: hydrophobic, hydrophilic) The KV1.1 protein utilizes __ transport to move the K+ ions across the membrane.  (word bank: active, passive) This disease can be caused by missense mutations.  In general, how specifically would a missense mutation affect the KV1.1 protein?

Order Ca2+, CO2, ethanol, glucose, RNA, and H2Oaccording to their ability to diffuse through a lipid bilayer,beginning with the one that crosses the bilayer most read-ily. Explain your order.

6. You discover a new transmembrane ion channel and upon structural characterization you demonstrate that it is a homo-tetramer, in which each subunit possess 2 transmembrane a- helices spanning the entire length of the membrane. Both of these helices are perfectly perpendicular to the plane of the membrane and 20 amino acid residues are embedded in the lipid bilayer. a. Estimate the thickness of the membrane lipid bilayer. b. If the homotetramer has MW of 70 KDa, what percent of its mass would reside in the membrane-spanning portion? c. Upon conformational change one of the two helices in each subunit tilts to 45 degrees within the membrane in respect to the plane. How many amino acids in this helix are embedded in the membrane after the transition? d. What percent of the ion channel mass reside in the membrane-spanning portion after the conformational change occurs? e. Transmembrane pores and channels can also be formed by proteins that form B-barrel structures. B-barrels are…

Fat is stored in adipose cells in the body, and glycoproteins are produced by liver cells. Which of the following is likely to be more prominent in adipose cells than in liver cells? A B с D cell membrane smooth endoplasmic reticulum nucleus rough endoplasmic reticulum

The image below represents a patch of membrane, in which there are embedded some proteins with sugars attached to them. Please answer these two questions: 1. Describe at least one function that these sugars may serve to this cell in the figure. 2. Describe how/where (in the cell) these sugars must have been attached to these membrane proteins.

Your research advisor would like you to measure the movement of a protein X using FRAP. The results of your FRAP experiment are shown below. What can you conclude? Fluorescence Intensity (Arbitrary Units) Time (Seconds) Time point of exposure to an intense laser Protein X appears to be completely immobilized in the membrane Protein X appears to be partially immobilized in the membrane You cannot make predictions about the mobility of Protein X with these data Protein X appears to be completely mobile in the membrane

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