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1) Pretend you are investigating a newly seized tusk and wonder if the tusk is from a recently discovered crime where 10 elephants from the same population were found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently slaughtered elephants. C. a. Did the seized tusk come from one of the recently slaughtered elephants? If so, which one, or if no, why not? The seized fusks came from elephant #8. This is because the seized elephants matches Perfectly with the decreased, PCR. Since everyone has DNA that is unique, it was an obvious match- b. For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder that has DNA fragments that are 75, 125, 150, and 175 base pairs (bp) in size. Base Pairs 200 180 160 140 120 DNA Ladder Seized Tusk Matching Elephant dadong XAHA Seized Tusk Allele 1: Allele 2: to yonou Elephant 1 T able 1: Genotype for five STR loci recovered from a seized elephant tusk. Elephant 2 Elephant 3 Elephant 4 Elephant 5 Elephant 6 Elephant 7 Elephant 8 Elephant 9 FH19 FH60 FH67 FH71 147 97 62 147 103 62 Elephant 10 193 193 FH19 189 193 185 193 183 185 193 193 187 187 187 193 193 193 193 193 193 193 FH60 FH67 FH71 147 97 147 97 147 95 147 147 95 103 147 97 147 147 105 91 145 95 147 95 145 91 147 97 149 97 151 147 97 97 147 97 147 97 189 147 193 147 105 62 64 95 ៩ ៩ ៩៩៩ ៩៩ ៨ ៩ ៩ |8d| ថ ៨ ៩ ៩ ៩ 62 62 62 64 62 64 62 64 64 64 60 147 103 62 62 62 62 62 62 FH129 152 160 62 FH129 152 154 152 152 152 162 152 152 152 160 152 160 156 160 152 160 160 160 152 156 T Table 2: Genotypes using five STR loci for 10 slaughtered elephants. How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks?

1) Pretend you are investigating a newly seized tusk and wonder if the tusk is from a recently discovered crime where 10 elephants from the same population were found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently slaughtered elephants. C. a. Did the seized tusk come from one of the recently slaughtered elephants? If so, which one, or if no, why not? The seized fusks came from elephant #8. This is because the seized elephants matches Perfectly with the decreased, PCR. Since everyone has DNA that is unique, it was an obvious match- b. For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder that has DNA fragments that are 75, 125, 150, and 175 base pairs (bp) in size. Base Pairs 200 180 160 140 120 DNA Ladder Seized Tusk Matching Elephant dadong XAHA Seized Tusk Allele 1: Allele 2: to yonou Elephant 1 T able 1: Genotype for five STR loci recovered from a seized elephant tusk. Elephant 2 Elephant 3 Elephant 4 Elephant 5 Elephant 6 Elephant 7 Elephant 8 Elephant 9 FH19 FH60 FH67 FH71 147 97 62 147 103 62 Elephant 10 193 193 FH19 189 193 185 193 183 185 193 193 187 187 187 193 193 193 193 193 193 193 FH60 FH67 FH71 147 97 147 97 147 95 147 147 95 103 147 97 147 147 105 91 145 95 147 95 145 91 147 97 149 97 151 147 97 97 147 97 147 97 189 147 193 147 105 62 64 95 ៩ ៩ ៩៩៩ ៩៩ ៨ ៩ ៩ |8d| ថ ៨ ៩ ៩ ៩ 62 62 62 64 62 64 62 64 64 64 60 147 103 62 62 62 62 62 62 FH129 152 160 62 FH129 152 154 152 152 152 162 152 152 152 160 152 160 156 160 152 160 160 160 152 156 T Table 2: Genotypes using five STR loci for 10 slaughtered elephants. How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Please solve question b) and c) * The seized tusks came for elephant #8.
1) You are investigating a newly seized tusk to determine if it is from a crime scene where 10 elephants from the same population were found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently slaughtered elephants. **a.** Did the seized tusk come from one of the recently slaughtered elephants? If so, which one, or if not, why not? The seized tusk came from elephant #8. This is because the seized elephant’s matches perfectly with the deceased PCR. Since everyone has DNA that is unique, it was an obvious match. **b.** For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder with DNA fragments that are 75, 125, 150, and 175 base pairs (bp) in size. (Graph Explanation: A diagram showing a DNA ladder with base pairs marked at 75, 125, 150, and 175. Alongside, bands for the seized tusk and matching elephant are drawn to illustrate the alleles.) **c.** How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks? The pattern of bands in the gel supports that the seized tusk came from elephant #8 because the band sizes (alleles) perfectly match between the seized tusk and elephant #8 at all five loci. --- **Table 1:** Genotype for five STR loci recovered from a seized elephant tusk. - **Allele 1:** 193, 147, 97, 62, 152 - **Allele 2:** 193, 147, 103, 62, 160 **Table 2:** Genotypes using five STR loci for 10 slaughtered elephants. - **Elephant 1:** 189, 147, 97, 62, 152; 193, 147, 97, 64, 154 - **Elephant 2:** 185, 147, 95, 62, 152
Transcribed Image Text:1) You are investigating a newly seized tusk to determine if it is from a crime scene where 10 elephants from the same population were found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently slaughtered elephants. **a.** Did the seized tusk come from one of the recently slaughtered elephants? If so, which one, or if not, why not? The seized tusk came from elephant #8. This is because the seized elephant’s matches perfectly with the deceased PCR. Since everyone has DNA that is unique, it was an obvious match. **b.** For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder with DNA fragments that are 75, 125, 150, and 175 base pairs (bp) in size. (Graph Explanation: A diagram showing a DNA ladder with base pairs marked at 75, 125, 150, and 175. Alongside, bands for the seized tusk and matching elephant are drawn to illustrate the alleles.) **c.** How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks? The pattern of bands in the gel supports that the seized tusk came from elephant #8 because the band sizes (alleles) perfectly match between the seized tusk and elephant #8 at all five loci. --- **Table 1:** Genotype for five STR loci recovered from a seized elephant tusk. - **Allele 1:** 193, 147, 97, 62, 152 - **Allele 2:** 193, 147, 103, 62, 160 **Table 2:** Genotypes using five STR loci for 10 slaughtered elephants. - **Elephant 1:** 189, 147, 97, 62, 152; 193, 147, 97, 64, 154 - **Elephant 2:** 185, 147, 95, 62, 152
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