1) Pretend you are investigating a newly seized tusk and wonder if the tusk is from a recently discovered crime where 10 elephants from the same population were found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently slaughtered elephants. C. a. Did the seized tusk come from one of the recently slaughtered elephants? If so, which one, or if no, why not? The seized fusks came from elephant #8. This is because the seized elephants matches Perfectly with the decreased, PCR. Since everyone has DNA that is unique, it was an obvious match- b. For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder that has DNA fragments that are 75, 125, 150, and 175 base pairs (bp) in size. Base Pairs 200 180 160 140 120 DNA Ladder Seized Tusk Matching Elephant dadong XAHA Seized Tusk Allele 1: Allele 2: to yonou Elephant 1 T able 1: Genotype for five STR loci recovered from a seized elephant tusk. Elephant 2 Elephant 3 Elephant 4 Elephant 5 Elephant 6 Elephant 7 Elephant 8 Elephant 9 FH19 FH60 FH67 FH71 147 97 62 147 103 62 Elephant 10 193 193 FH19 189 193 185 193 183 185 193 193 187 187 187 193 193 193 193 193 193 193 FH60 FH67 FH71 147 97 147 97 147 95 147 147 95 103 147 97 147 147 105 91 145 95 147 95 145 91 147 97 149 97 151 147 97 97 147 97 147 97 189 147 193 147 105 62 64 95 ៩ ៩ ៩៩៩ ៩៩ ៨ ៩ ៩ |8d| ថ ៨ ៩ ៩ ៩ 62 62 62 64 62 64 62 64 64 64 60 147 103 62 62 62 62 62 62 FH129 152 160 62 FH129 152 154 152 152 152 162 152 152 152 160 152 160 156 160 152 160 160 160 152 156 T Table 2: Genotypes using five STR loci for 10 slaughtered elephants. How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks?

DNA strands are so tiny that they cannot be seen under a microscope; however, there is a technique to determine them without touching or seeing them. Gel electrophoresis is the name given to this technique. This method is also useful for separating molecules such as proteins.Answer b: 1 band for STR loci----- 147bp for FH 60 For FH129----- 2 bands of 152 bp and 160 bp will be observed Hence, Well, 1 ---- have seized tusk DNA Well 2 ---- investigating the elephant DNA We discovered that well 1 bp corresponds identically with well 2 bp, indicating that the allele identified for the stolen tusk and elephant has a probable match for STR loci FH60 of FH129.Answer c: Here, data from table 1 matches data from elephant 8 in table 2, indicating that the seized tusks originated from a freshly slaughtered elephant.