1) One of the most common buffers used in biological applications is Tris H*/Tris: LOH HO но. OH HO. OH NH, NH2 Tris H* (K, = 7.94 x 10) Tris la) What is the pH of 0.75 L of Tris buffer containing 13 mmol Tris H* and 11 mmol Tris? lb) You add 0.020 L of 0.15 M HCI to the exact buffer solution from part (a). What is final pH? Partially filled ICE table provided for convenience, units in mmol Tris H H Tris 13 11 C To a fresh 0.75L portion of Tris buffer identical to that in part (a) you add 0.10 L of 0.15 M HCI. lc) What is final pH? Partially filled ICE table provided for convenience, units in mmol Tris H* H Tris 13 11 E

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1) One of the most common buffers used in biological applications is Tris H*/Tris:
HO
HO
но.
HO.
HO
NH3*
NH2
Tris H* (K, = 7.94 x 109)
Tris
la)
What is the pH of 0.75 L of Tris buffer containing 13 mmol Tris H* and 11 mmol Tris?
lb)
Partially filled ICE table provided for convenience, units in mmol
You add 0.020 L of 0.15 M HCI to the exact buffer solution from part (a). What is final pH?
Tris H*
H
Tris
13
11
E
To a fresh 0.75 L portion of Tris buffer identical to that in part (a) you add 0.10 L of 0.15 M HCI.
lc)
What is final pH?
Partially filled ICE table provided for convenience, units in mmol
Tris H
Tris
H
13
11
E
Transcribed Image Text:1) One of the most common buffers used in biological applications is Tris H*/Tris: HO HO но. HO. HO NH3* NH2 Tris H* (K, = 7.94 x 109) Tris la) What is the pH of 0.75 L of Tris buffer containing 13 mmol Tris H* and 11 mmol Tris? lb) Partially filled ICE table provided for convenience, units in mmol You add 0.020 L of 0.15 M HCI to the exact buffer solution from part (a). What is final pH? Tris H* H Tris 13 11 E To a fresh 0.75 L portion of Tris buffer identical to that in part (a) you add 0.10 L of 0.15 M HCI. lc) What is final pH? Partially filled ICE table provided for convenience, units in mmol Tris H Tris H 13 11 E
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