1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µl as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 pl of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of DH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µl 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram

1) Make up the following standard samples NOTE: when measuring the volumes to make the bellow samples, use only one pipette/volume rounding it to the closet setting to the required volume (do not measure 247.5 µl as 240 µL and 7.5 µL, rather measure it as 248 µL) a. 247.5 pl of dH2O + 22.50 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) b. 258.75 µl of DH2O + 11.25 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) c. 262.5 µL of dH2O + 7.5 µL Stock BSA (5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) d. 232.5 µL of dH2O + 37.5 µL 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) e. 251.25 µl of dH2O + 18.75 µl 1/10 BSA (0.5 µg/µL) + 30.0 µL 0.25 M Tris (pH 7.5) please help me determine (show calculation) the BSA loaded in microgram

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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