.columns with one million theoretical plates operating by slip flow. The figure shows the gradient separation of two forms of fluorescently labeled bovine serum albumin separated on a)50 x 2.1 mm UHPLC column of 1.7 µm C4 silica particles and b)10 mm x 75µm colloidal crystal column of 470 nm C4 silica nanoparticles. What are the main differences in the chromatogram? What is the reason for the difference? Why does the colloidal crystal column have such a high number of theoretical plates (N)? How is the problem of high pressures with very small particles overcome?
.columns with one million theoretical plates operating by slip flow. The figure shows the gradient separation of two forms of fluorescently labeled bovine serum albumin separated on a)50 x 2.1 mm UHPLC column of 1.7 µm C4 silica particles and b)10 mm x 75µm colloidal crystal column of 470 nm C4 silica nanoparticles. What are the main differences in the chromatogram? What is the reason for the difference? Why does the colloidal crystal column have such a high number of theoretical plates (N)? How is the problem of high pressures with very small particles overcome?
Chapter6: Random Errors In Chemical Analysis
Section: Chapter Questions
Problem 6.13QAP
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4.columns with one million theoretical plates operating by slip flow.
The figure shows the gradient separation of two forms of fluorescently labeled bovine serum albumin separated on
a)50 x 2.1 mm UHPLC column of 1.7 µm C4 silica particles and
b)10 mm x 75µm colloidal crystal column of 470 nm C4 silica nanoparticles.
- What are the main differences in the chromatogram? What is the reason for the difference?
- Why does the colloidal crystal column have such a high number of theoretical plates (N)?
- How is the problem of high pressures with very small particles overcome?
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