. What would be the effect of the following errors in chromatographic work? a. The solvent level in the developing chamber is higher than the spotted sample.  b. Too much sample is applied to the paper. c. The paper is allowed to remain in the chamber after the solvent front has reached the top of the plate.

1. What is the purpose of adding immersion oil when using the 100X objective?

Which of the following specimens cannot be tested on the Velocity Macroscopic Chemistry Module? a. Gross Hematuria b. Specimens <2 mL c. Pyridium d. Diluted Specimens e. All of the above

4. TLC is still useful in the lab but is being replaced by automated chromatographic methods. For example, TLC, FPLC, and HPLC are now automated processes, and computers can control various parameters, such as flow rate, blends of solvent mixtures, etc. Can you think of how the ability to manipulate the parameters of these automated chromatographic methods (sample size, column plate length, flow rate, solvent gradients, etc.) might accentuate or improve an investigator's ability to separate amino acids or other biomolecules?