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Yeast Fermentation Lab Report

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Brittney Tokarick
Dr. Yorty
Bio111 – Lab
November 5, 2014
CO2 Evolved due to Temperature’s Effect on Yeast Fermentation of Glucose
Abstract
This experiment measured the amount of CO2 evolved based on yeast fermentation of glucose. It was hypothesized that at room temperature, about 22 degrees Celsius, less CO2 would be produced than when tested at 37 degrees Celsius. In order to determine the amount of CO2 evolved, an experiment was set up involving yeast, glucose, deionized water, and heat. To begin the experiment, six fermentation flasks were acquired. Six milliliters of 10% glucose was pipetted into each flask, followed by 10 mL of deionized water and 8 mL of the prepared yeast solution. After each flask was placed at the appropriate temperatures necessary for the experiment, the production of CO2 started to be monitored. Data was recorded consecutively every two minutes over a time period of twenty minutes, and recorded in a data chart. The average CO2 production at 37 degrees Celsius and 22 degrees Celsius was determined from the t-test results. According to these results, the average amount of CO2 produced after twenty minutes at 37 degrees Celsius was 123 millimeters, and the average CO2 produced at 22 degrees was 26 millimeters. These results proved the hypothesis as more …show more content…

In this experiment, the rate of fermentation was increased while at higher temperatures due to the increased rate of enzymatic activity. Enzymatic activity due to the level of kinetic energy also plays a significant role in the rate of yeast fermentation of glucose (Gale, T., 2006). Given that enzymes are more active at higher temperatures, this means that fermentation occurs at higher temperatures as well. Since heat causes enzymes to become more active, naturally more CO2 was produced over time at 37 degrees rather than at 22

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