Brittney Tokarick
Dr. Yorty
Bio111 – Lab
November 5, 2014
CO2 Evolved due to Temperature’s Effect on Yeast Fermentation of Glucose
Abstract
This experiment measured the amount of CO2 evolved based on yeast fermentation of glucose. It was hypothesized that at room temperature, about 22 degrees Celsius, less CO2 would be produced than when tested at 37 degrees Celsius. In order to determine the amount of CO2 evolved, an experiment was set up involving yeast, glucose, deionized water, and heat. To begin the experiment, six fermentation flasks were acquired. Six milliliters of 10% glucose was pipetted into each flask, followed by 10 mL of deionized water and 8 mL of the prepared yeast solution. After each flask was placed at the appropriate temperatures necessary for the experiment, the production of CO2 started to be monitored. Data was recorded consecutively every two minutes over a time period of twenty minutes, and recorded in a data chart. The average CO2 production at 37 degrees Celsius and 22 degrees Celsius was determined from the t-test results. According to these results, the average amount of CO2 produced after twenty minutes at 37 degrees Celsius was 123 millimeters, and the average CO2 produced at 22 degrees was 26 millimeters. These results proved the hypothesis as more
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In this experiment, the rate of fermentation was increased while at higher temperatures due to the increased rate of enzymatic activity. Enzymatic activity due to the level of kinetic energy also plays a significant role in the rate of yeast fermentation of glucose (Gale, T., 2006). Given that enzymes are more active at higher temperatures, this means that fermentation occurs at higher temperatures as well. Since heat causes enzymes to become more active, naturally more CO2 was produced over time at 37 degrees rather than at 22
There are many substances that can be manipulated and cause the rate of reaction in fermentation to either speed up or slow down. Substances that alter the rate of the reaction could be temperature of the water, the yeast concentration, pH, and the glucose concentration. In the experimental group of the experiment the amount of yeast concentration was manipulated. The objective of this experiment was to determine what factors affect the rate of the fermentation. To test this objective we changed the amount of yeast being used. A higher yeast concentration replaced the controlled yeast amount. A prediction made by my group was that higher amount of yeast would speed up the process of fermentation. Our null hypothesis is there will be no
First I will set up the apparatus as show above. I will add 1.5 grams
Yeast is a fungus that can generate glucose into energy without using any oxygen molecules. We tested the fermenting ability of yeast from two different carbon sources: glucose and aspartame. We hypothesized that yeast is unable to use the carbon sources of aspartame. To do this, we decided to use both carbon sources in the same concentration. Each carbon source was mixed with the same amount of yeast solution. The experiment group of 5.5 mM aspartame solution was compared with the control group of 5.5mM glucose solution. We recorded the rate of fermentation for glucose and aspartame in the Vernier Lab Quest. The fermentation rate of aspartame is a negative number, and glucose is a positive number. Our results show that yeast was unable to ferment aspartame as yeast fermented glucose. The results indicate that aspartame has no effect on yeast fermentation rate because yeast do not catabolize aspartame because it does not have the appropriate enzymes to break it down.
Abstract: This lab’s purpose was to see how different levels of yeast, distilled water, and sugar interact to affect the level of carbon dioxide evolved in fermentation. In this experiment we had two sections. The first section tested four test tubes with varying levels of yeast, glucose and distilled water for evolved carbon dioxide levels. The tubes were timed for 20 minutes. The amounts of solution in the test tubes are noted in the methods section of this lab report. The second section of the lab used three test tubes and flowed the same procedure except added spices. The levels of ingredients are also in the methods section. The main goal of this experiment was to see the effects of yeast concentration.
PH can affect the way fermentation occurs due to the irregularity of the acidity or alkalinity within the glucose solution. This is an enzyme-based reaction that is susceptible to pH. The aim of this experiment was to determine how pH affects the yeast fermentation rate by performing the experiment numerous times with a different pH of glucose solution which included pH 3, 5, 7, 9, 11. The hypothesis was ‘If the pH is lower than the neutral point then the fermentation reaction will occur faster?’ The experiment conducted was to measure the amount of C02 produced by the yeast going into fermentation, however varying the pH of glucose solution by using different pHs . To test this every 5 minutes the volume of gas in the test tube was observed and recorded until a period of 30 minutes had been. The end results
For the experiment, the changes of temperature on anaerobic fermentation the process in which cells undergo respiration without oxygen in Saccharomyces cerevisiae was observed. The purpose of this experiment was to test the effect of four different temperatures on the rate of carbon dioxide production in yeast by measuring the fermentation rate. Saccharomyces cereviviae, also known as Baker 's yeast, is a unicellular, eukaryotic sac fungus and is good for this experiment because of its characteristic of alcohol fermentation. It was hypothesized that fermentation increases with increased temperature to a point of 37°C; above that point, enzyme denaturing will occur and fermentation will decrease. The group was able to document the carbon dioxide production and mark each of the temperature intervals which were tested at temperatures 4°C (refrigerator temperature), 23°C (Room temperature), 37°C (Human body temperature) and 65° Celsius (Equal to 150°F). The experiment was conducted by pouring yeast solution with 2% glucose in fermentation tubes, placing the tubes in the appropriate incubation temperature, marking the rise of the gas bubbles in the fermentation tubes which indicated carbon dioxide production. The results of this experiment were not supported by the hypothesis, creating different results from what was predicted. It is important to understand the fermentation rate of yeast so
1. Lab reports are to be computer-generated and double-spaced. All sections of the report must
There are many processes that are needed to occur to produce something that help organisms live. Cellular respiration and fermentation are two process that are important to the survival of organisms. Cellular respiration is the way cells make ATP, which they need to survive. The process starts with the breaking down of glucose into other compounds that can be used by the cell. However, there are more steps in the process than just cellular respiration and how precise cellular respiration is depends on how much ATP can be taken from food particles in the body (Hill 646). Fermentation is mostly known in the world of beer and wine, but it also produces lactate in organisms. Fermentation is breaking glucose into separate components like water or carbon dioxide, much like that of cellular respiration. N’guessan and some peers did an experiment and they found that after fermentation had stopped, they had over 200 counts of yeast in the beer (N’guess, Brou, Casaregola, Dje 858). Under the
Hypothesis: The yeast will have a greater rate of respiration for the natural sugars specifically the glucose and lower rate of respiration for the unnatural sugars
The purpose of this investigation is to test the effect of different sugar sources on yeast respiration.
The objective of the experiment is to test the effect of five different temperatures on the rate of carbon dioxide production in yeast. In order to achieve this, five fermentation tubes are filled with 30 ml of yeast and a sugar culture. The tubes are then added to water baths of varying temperatures to determine which one yields the most carbon dioxide. Results support the conclusion that yeast will produce carbon dioxide most efficiently in a setting with a temperature at or about 45°C.
This lab investigates the effects of Sucrose concentration on cell respiration in yeast. Yeast produces ethyl alcohol and CO2 as a byproduct of anaerobic cellular respiration, so we measured the rate of cellular respiration by the amount of CO2
The procedure for this experiment was to first obtain four balloons and blow them up in order to stretch them. Then obtain and fill the four large test tubes each with thirty milliliters of warm forty degrees Celsius water and two grams of dry yeast which was weighed on a scale and scooped out by a spatula. After five milliliters of water, ten percent glucose, fructose or sucrose went into one of the four test tubes. Then parafilm was placed on top of each of the test tubes to seal them and they were swirled activating the yeast through rehydration. After swirling the film was removed and the balloons were tightly placed on the test tubes. Then finally observed the tubes build up of CO2 all the while swirling gently every fifteen minutes, recording observations.
Anaerobic fermentation is a process that produces energy after glycolysis occurs if oxygen is not present or available. There are two types of anaerobic fermentation: alcoholic fermentation and lactic acid fermentation. Alcoholic fermentation is a process that converts sugars (pyruvic acid) into ethanol and carbon dioxide. This process occurs in the cytoplasm of yeast (fungus) cells and allows bread to rise and bubbles to form in champagne when introduced to specific temperatures. The purpose of this lab was to determine the effect of temperature on the rate of alcoholic fermentation. The hypothesis was that a higher temperature will allow the rate of fermentation to occur faster. The independent variable was the temperature the yeast was exposed to, and the dependent variable was the rate of fermentation. The experimental group was the test tube introduced to the room temperature beaker (Beaker C). The control group were the test tubes that were introduced to the hot and cold beakers (Beaker A and B). The constants in this experiment were the experiment took place,
4) One package of active dry yeast was added to bottle labeled ‘5mL’ and solution was swirled.