Why Does The Pigment Appear Green?

In order to obtain the photosynthetic pigment’s absorption spectra the pigments are separated using paper chromatography. Paper chromatography is an analytical technique that separates a mixture based on the individual pigment’s size, polarity and solubility (Lewis, 2004). The separation of the mixtures involves a stationary phase (the chromatography paper), which a mobile phase (solvent) moves up through. When the mixtures is applied to the paper and allowed to flow with the mobile phase, the different pigments move at different rates (Campbell, 1996). This means the pigments that absorb the strongest to the stationary phase (the chromatography paper) will move the slowest, while the weakest will move the fastest. The rate of the pigments movement will separate each pigment individually from the mixture (Maitland, 2002). This natural separation shows that each pigment is chemically different and plays different roles in photosynthesis (Maitland, 2002).

Purpose: The purpose of this lab is to identify the dyes present in the different mixtures. Introduction: This lab uses chromatography to determine the dyes present. It is also used to identify the different intermolecular forces each solution and dye has.

Once the application of the spinach extract was finished, acetone, the solvent, was poured into the beaker. Then the cylinder was placed into the beaker to absorb the solvent with a jar top placed on top of it to hold it in place. The chromatography cylinder stayed in the beaker until the pigments in the spinach extract had been separated into different colors and was about 4-5 cm from the top of the paper. After this, the chromatography cylinder was removed to dry for the different pigments could be observed.<p>

To determine the effect of light on CO2 production for photosynthesis, the amount of light an Elodea plant received was inhibited with a mesh cloth while a second beaker of the same Elodea plant was exposed to the full light intensity. The purpose of this experiment was to then compare and determine if CO2 production would be decreased if direct light was inhibited.

In Scenario 4, two samples of Ivy were tested because two students, Malik and John disagreed on how plant pigments can be analyzed. Malik and John argue that the ivy in the sun has varied pigments while the ivy in the shade has green pigments. To settle Malik and John’s argument, a leaf from the shaded ivy were collected, and a leaf from the ivy growing in the sun was collected. To test these samples the pigments of the ivy in the sun and the pigments of the ivy in the shade were extracted to test the absorption level of each ivy sample. The samples were also tested for the determination of the absorption spectra of each pigment extract.

Paper chromatography will be used to separate the pigments from spinach extract. All chromatography has two phases: the stationary phase or the absorbant, which is the chromatography paper, and the mobile phase or the eluent, which is the developing solvent in the jar. The solvent used for this lab was nonpolar mixture of petroleum ether and acetone; while the chromatography paper was polar. Since the polarities of these substances are opposite, the spinach extract would either move up the chromatography paper along with the solvent to the solvent front, or be attracted to the chromatography paper and stay where the extract was placed

We were also careful about the concentration of the extract, as the transmittance of the extract at first should be between 65% to 85%; when it is under 65% the solution is too concentrated and some ethanol should be added, and when it is greater than 85% it is not concentrated enough. Another crucial point was placing the cuvette of ethanol into the sample compartment and calibrating to 100% transmittance using the '100%T/0A' knob prior to placing the cuvette of extract, as it is essential to zero the absorbance every time before qualifying the absorbance of the chlorophyll pigments. With special attention to these details, the aim of the experiment was achieved.

Photosynthesis is a process used by plants and other living organisms that use light energy from the sun into chemical energy that can later be used as energy for other organisms. Numerous factors that influence the rate of which photosynthesis occurs, which includes temperature, amount of light and carbon dioxide. The aim of the experiment was to investigate the effect of increasing concentration of carbon dioxide on the rate of photosynthesis. For example, to test the hypothesis, the effect of changing concentrations of carbon dioxide on the rate of photosynthesis in leaf discs was measured. This was done by removing air bubbles from the leaf discs, which caused the disc to sink to the bottom of the syringe and by placing them in different concentrations of sodium bicarbonate. The results on the graph started to decrease as the concentration level of the sodium bicarbonate was increased. A summary of errors occurred from some leaf discs floating before the experiment began (at time zero). However, results of the experiment supported the hypotheses, even though there were several errors

The purpose of this experiment is to determine the specific pigments that are found in each of the chosen plant leaves, as well as, discover the intermolecular forces present in each leaf. This is accomplished by using a technique called chromatography, which splits a mixture or solution into its different parts based on the mixtures ability to dissolve in a chosen solvent. Chromatography works by placing a strip of chromatography paper, that has a small amount of mixture on it, into a cup of the specific solvent chosen. In this experiment, one hundred percent acetone was chosen to be the solvent. Once the small amount of the mixture is aligned with the solvent, the mixture will begin to separate and rise by capillary action based on the solubility,

Abstract Photosynthesis is the primary focus of this experiment. We did a couple different tests in order to find out how the pigments in plants make photosynthesis possible. Paper chromatography was carried out in order to find out the solubility and polarity of each pigment we tested. Also each pigment was put in spectrophotometer to measure the absorption and wavelengths. Out of the four pigments we tested, our accessory pigment β-

This experiment focus on pigments quantification from chloroplast. Pigments are extracted from frozen chloroplasts extracted from barley leaves. Dilution was made using buffer. In order to get final concentration acetone (100%) was added into each dilution. Thereafter total concentration of pigments is determined

In addition to water and carbon dioxide, photosynthesis requires light and chlorophyll, a molecule in chloroplast.. Plants gather the sun's energy with

The blank, which was used in this lab, was acetone. Test tube a contained chlorophyll a and test tube b contained chlorophyll

Introduction Plants give us some of the oxygen we breath, they give us our food through agriculture, and they keep soil in place with their roots. Plants go through many different cycles to get the nutrients that they need to survive. They have to go through a process called photosynthesis to create the nutrients that they need survive. Photosynthesis is the process by which some autotrophic organisms use sunlight to synthesis foods from carbon dioxide and water, as stated in the Dictionary. In order to create food through photosynthesis they need chloroplast which gives plants their green pigment.

The spectroscopy lab was conducted to demonstrate the characteristics of different solutions using multiple methods. The different methods used in this lab for characteristic determination was spectroscopy to determine absorbance spectrums and concentration curves of different solution and chromatography was used to separate the chlorophyll extract solution into its individual pigments to determine its components. The first and second experiment used the spectrometer to determine absorbance values which would be input into absorption vs. wavelength graph and the concentration vs. absorbance graph. Ultraviolet spectroscopy (method used in lab) is used to determine the movement and the various properties of a molecule and its interaction with other molecules using ultraviolet light absorption (Demchenko, 2).