Unknown Lab Report

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

The unknown bacteria plate chosen was plate #2. It was identified to be Micrococcus luteus. It is a gram positive, Coccus bacteria that is commonly found in dust, water, soil, and the air. M. luteus also thrives in the human mouth and upper respiratory tract. Sir Alexander Fleming discovered it in 1928 before he identified penicillin. It is part of the normal flora on human skin as well as other mammals. Since it is part of normal flora it is normally not pathogenic, but can become opportunistic in an immune-deficient person. It has been known to cause septic shock, UTI’s, and even pneumonia. Micrococcus luteus is both urease and catalase positive. It does not utilize tryptophan for indole production. It is a facultative anaerobe. Mobility is not present for this bacterium. Starch is also not hydrolyzed and oxidase is not present.

This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.

A sample of urine was taken from a patient with kidney disease was labeled as

The goal of the experiment was to identify Unknown 33A and 33B. Unknown 33A was a white, crystalline solid that had a sweet cherry smell and Unknown 33B was a beige, yellowish color liquid that was translucent and had a viscosity similar to water. Also, the liquid was homogenous and smelled sour, similar to mildew.

To be able to classify bacterial unknowns through identification techniques for medical and research fields is a crucial method. It can be used for a range of investigations, from being able to pinpoint the underlying cause of a disease to knowing if a certain ecosystem is cycling properly. These identification methods are used regularly in medical and research fields. There are various identification methods that select, differentiate, and cultivate microbes. These include using various types of mediums and biochemical tests.

Aim: To classify unknown substances according to their structure type and to observe how the structure of materials affects their uses.

The results that I observed by Gram Staining was Gram Negative rod (bacillus). Then, I performed a Negative Stain in order to further more validate that I had bacillus, since Negative Staining would provide an accurate determination of the shape of the microbe by staining the background of the microbe. In observation of my unknown using Negative Staining; I knew that I had a microbe with bacillus.

We then observed the slide to see if a reaction took place. The next test we performed distinguished gram-positive bacteria depending on their salt tolerance. We tested for growth on Mannitol Salt Agar (MSA). A live culture was prepared using water and a sample of our bacteria from an isolated colony. We then spread the cells across the agar on the MSA plate, and incubated the Petri dish at 37°C for 2 days.

In this experiment, each student was randomly assigned with a different species of gram- negative bacteria. The organism that I was assigned was Unknown #16. The identity of the gram-negative bacteria was determined to be Escherichia coli. The purpose of this report is to describe the various tests that helped develop a better understanding of the unknown microorganism in terms of the physiology, morphology, motility, and antimicrobic sensitivity it is characterized with. Indole production, hydrogen sulfide, and the colony morphology on the Eosin-methylene blue (EMB) plate, were the critical results that led to the conclusion that the organism was E. coli. In the indole production test, E. coli was one of two organisms,

-B2 was Gram positive cocci that seemed to cluster and also showed no growth on the EMB plates.

The pH of saliva was 7 this means that it is neutral and there is a large amount mineral reserves, unlike when it is 6.25 to 6.75 which indicates that there is an excess of acid and that the there is a very low amount of mineral reserves and that on its own is not healthy. The saliva contributes to supporting the pH by two components. To begin with, the flow of saliva kills starches that could be utilized by microorganisms and evacuates acids created by microscopic organisms (Baliga et al, 2013). Second, acidity from beverages and nourishments, as well as bacterial activity, is neutralized by the buffering action of salivation (Baliga, 2013). The solution that was mixed with water and Biuret test reagents was clear because there was no protein presence,

CE3.13 Since I had previous experiences in using the survey software, I recommended the usage of Ekahau site survey. Even though there are different survey tools were available in the market, the reason I selected this tool is that it facilitates WLAN planning at the pre-deployment stage. I advised Mr. Surya, who was the lead systems engineer, to use of this tool for the detection of the coverage holes and co-channel interference zones in the various location of campus. Ekahau wireless adapter was used to analyse the coverage holes and Ekahau site survey software was used to monitor the coverage holes. Heat maps to detect the holes in coverage and analysed the coverage distribution in software tools. Since this project was aimed to cover

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Mahan, C.R. Lehman, D.C. and Manuselis, G. (2014). Textbook of Diagnostic Microbiology. 5ed. Elesevier. UK.