Experiment Results Predict Questions: Metabolic Rate Of Rat

f. The student is conducting a nutrition experiment using lab rats. One group (control group) is fed a normal diet, while the other group (experimental group) is fed a diet high in protein. There were 30 rats total used in the experiment. At the end of the experiment, the average mass (weight) of a rat in the control group was 60.4 g while the average mass (weight) of a rat in the experimental group was 0.0689 kg. What is the average difference in weight between the two groups in grams?

| | | |Males: Calories Expended for Body Function(6.27 x weight in lbs.) + (12.68 x height in inches) - (6.76 x age) + 66| |

The purpose of this lab is to gain knowledge and understanding of the scientific principle of RER, using indirect calorimetry to measure caloric expenditure, including the caloric equivalent and to understand the concepts behind estimating oxygen consumption and caloric expenditure from heart rate, including the limitations. In addition the purpose was to learn skills of how to measure and graph the linear relationship between heart rate and VO2 and then determine the regression equation line and to estimate the caloric expenditure from VO2, heart rate, and caloric equivalent values.

To determine the metabolic rate of a goldfish two different methods can be applied, direct or indirect calorimetry. Direct calorimetry analyzes the exothermic reaction when ATP is produced by measuring the amount of heat that is released. Meanwhile, indirect calorimetry measures the amount of carbon dioxide or oxygen because both are components of aerobic respiration, a process which repeatedly supplies more ATP to match the demands of metabolic rate of an organism. Evidently, metabolic rate is the cumulative sum of energy used by all the cells. Most of this energy comes from regulating homeostasis, locomotion and thermoregulation. On the other hand, ectotherms like goldfish have a slight difference in their metabolic rates because their internal temperature directly correlates with the temperature of their environment. For this reason, ectotherms use less energy because they do not need to worry about thermoregulation, maintaining constant body temperature. However, temperature, size, amount of light and stimulus are factors that can affect metabolism of goldfish. Thus, this experiment will measure the metabolic rate of goldfish through in

Breathing Rate 2.6 2.9 3 2.8 TV(L) 2.9 3 2.9 2.9 Resting Values ERV(L) IRV(L) 3.9 4.3 4.3 4.2 5.5 5.9 5.9 5.8 RV(L) 3.4 3.6 3.7 3.6 Breathing Rate 2.2 2.3 2.3 2.3 TV(L) 4 4.3 4.4 4.2 Exercising Values ERV(L) IRV(L) 5.6 5.9 6 5.8 6.2 5.3 6.7 6.1 RV(L) 42.2 50.2 49.5 47.3

Liver is known as the metabolic port of entry for any endobiotic and xenobiotic substance. The anabolism and catabolism of most of the nutrients are in major performed by the liver. Carbohydrate, protein and fat metabolism by the liver is of significance as the anomalies in metabolism of these nutrients may introduce several types of metabolic syndromes. The protective effects of aqueous green tea extract have been shown on hyperglycaemia, hyperlipidaemia and liver dysfunction in diabetic and obese rat models [74]. Different nutrients combinations were tested in a long-term feeding in experimental mouse model. Regional and continental food habit and practices are very much different. Effects of Western, vegetarian, and Japanese dietary fat

Hashimoto’s Disease is a chronic Lymphocytic thyroiditis also known as autoimmune thyroiditis. It is due to chronic inflammation; the disease is a failure of the thyroid gland. Hashimoto’s disease is the most common inflammatory disorder of the thyroid and the leading cause of having hypothyroidism. The name of the disease came from a Japanese surgeon named Hashimoto Hakaru. Hakaru first described the chronic disease as Struma Lymphomatosa in a pathology report in the year 1912. Hashimoto’s Disease is a genetic trait and found to be more common in women then in men; and commonly found in women between the ages of thirty to fifty years of age. Hashimoto’s Disease can be found in children too; in the first-degree relatives that have the disease is found to have an increased incident.

O B J E C T I V E S 1. To define the following terms: metabolism, hormone replacement therapy, type 1 diabetes, type 2 diabetes, and glucose standard curve. 2. To explain the role of thyroxine in maintaining an animal’s metabolic rate. 3. To explain the effects of thyroid-stimulating hormone on an animal’s metabolic rate. 4. To understand how estrogen affects bone density. 5. To explain how hormone replacement therapy works. 6. To explain how fasting plasma glucose is used to diagnose diabetes. 7. To understand how levels of cortisol and ACTH can be used to diagnose endocrine diseases.

In this task I will be examining how the different body systems use energy and why the body needs energy. I will also be analysing cellular respiration which is the process in which energy is made, along with the by-products and anabolism and catabolism. Later on in this task I will be analysing how ATP is used in muscle action, protein production and how we as mammals use the energy released as heat.

The topic of this lab is on biochemistry.This experiment was conducted to show how cells prevent the build of hydrogen peroxide in tissues. My group consisted of Lekha, Ruth, and Jason. There were used two different concentrations of hydrogen peroxide through this experiment , 1.5% and 3%. By testing two different types it is easier to understand how the H2O2 and catalase react with one another. To do this both the yeast, which was our catalase, and H2O2 were mixed together in a beaker. Each concentration was tested out twice for more accurate results . 1.5% concentrated H2O2 had an average reaction rate of 10.5 seconds while 3% concentrated H2O2 had an average reaction rate of 7.5 seconds. From this experiment we learned that by increasing the concentration of H2O2 and chemically combining it with a catalase it will speed up the reaction. Enzymes speed up chemical reactions . The independent variable in this experiment was the concentration of the H2O2. Some key vocabulary words are Catalase, enzyme, hydrogen peroxide ( H2O2), and concentration.

BMR = 655 + (4.35 x 159) + (4.7 x 67) – (4.7 x 31) = 1515.85 kcal/day

In subjects who have acclimatized to altitude over a period of more than 3 weeks, exercising at the

Put the 100 g of potato in 1 petri dish and 100 g of liver in another petri dish. Then put equal amounts of hydrogen peroxide for an equal period of time in both dishes. Drain the dishes and titrate them with potassium permanganate simultaneously. The numbers can now be compared using the steps in the sample calculation above in the lab report. In order to compare perform a potassium permanganate titration on pure hydrogen peroxide.

Diabetes is a lifelong disease that can affect both children and adults. This disease is the sixth leading cause of death in the United States. It claims about 178,000 lives each year. Type one diabetes, also known as insulin dependent diabetes mellitus, usually occurs in people less than thirty years of age, but it also may appear at any age. Diabetes is a very serious disease with many life threatening consequences, but if it is taken care of properly, diabetics can live a normal life.

Chronic diseases such as hyperthyroidism can be debilitating if not managed well. Hyperthyroidism is an overactive thyroid gland that secrets too much hormones throughout the body causing many different clinical manifestations within all systems. The thyroid gland is located right underneath the larynx also known as the voice box, and straddling the windpipe.