preview

The Use Of Crispe Technology In Wine Production

Decent Essays
Open Document

A mechanism of modification is by altering the genome of a type of plant to engineer improved plant strains. In some cases, these modifications are being done to an already successful product, such as wine. This is seen in production of various and new types of wine being produced from the new grape varieties created using CRISPR. Given that grapes are very underrepresented in research, this research has provided new information on the genomes of grapes. This is also important research, because wine is a commercial good found all throughout the world, so this research can be utilized to improve wine production in many places in the world. (1). The use of CRISPR in wine production includes CRISPR technology using the target genes from the …show more content…

(cite) Results Various data was collected from this experiment taken from four different sites on exons targeted for cleavage. This was to see if a CRISPR/Cas 9 construct can be used for initiating mutagenesis in grape leaves (6). The results indicated that there was some successful CRISPR/Cas 9 induced mutagenesis, but at low levels in the data collected from grape calli. The physical characteristics of the wild type grape plants were normal, but transgenic plants differed in the physical appearance of their leaves. The study included an image (figure 1) that showed that there were noticeable white and pale green spots on the plants leaves. This physical difference demonstrates that, in the leaves, there was a larger number of mutations. A trend found when analyzing these leaves were newer leaves measured lower for mutations than older leaves. (9). The number of successful mutation ranged greatly from plant to plant type. The plant altered by CRISPR/Cas 9 did show that the expression of Cas 9 has some effect on the level of mutations measured using western blot analysis (12). This study successfully demonstrated that CRISPR/Cas9 mutagenesis in grapes and that the frequency of mutagenesis is relatively stable and efficient. (13) This study also shows a lot of promise for future engineering of altered plants. This study also shows support that CRISPR and Cas 9 can be used to engineer a new plant type, giving scientists another mechanism of plant

Get Access