Da Ye Kang
Population Design Lab
Ms. Chow
6th May 2014
Research Question/ Aim
To investigate how the change of the temperature on the surface of a glass jar, in conditions of 18°C(±0.5),20°C, 22°C, 24°C, 26°C, 28°C , 30°C respectively, affects the population growth of Drosophila melanogaster for 17 days.
Independent Variable
Seven different temperature conditions of 1L glass jar: 18°C(±0.5), 20°C, 22°C, 24°C, 26°C, 28°C, 30°C.
Dependent Variable
The number of adult Drosophila melanogaster (±1 fly) in 1000mL(±0.5) jar after 17 days, yet not including unhatched larvae and pupa.
Background Investigation
Drosophila Melanogaster are ectothermic animals, in which belongs to large family of Drosophilae, which is found commonly near human habitation. Adult are about 1mm in length with slightly larger female. Adult D. Melanogaster has large red compound eyes with short antennae, and mouth for sopping up liquids. Their main source of food is herbivore, and they often lay egg on fruits. For these reasons, their population tend to peak during fall, and are considered as major pests.
The average life span of D. Melanogaster is found be 26 days for female and 33 days for a male. Drosophila has evolutionary features of having a temperature sensor within its body. Its ability to sense and regulate the body temperature has been a great field of interest among researchers, in order to reveal how such traits evolved. Drosophila is an easy model for investigation in many occasions,
The 792 Drosophila melanogaster mutation is known as guindo. Guindo or guinda is the spanish word for a deep red wine, burgundy or ox-blood color. The phenotypic differences between a WT D.melanogaster and a 792 MUT differ at their larval, pupal and adult stage. In the larval stage there was not any significant phenotypic differences noticed, only behavioral differences were apparent. The wandering MUT larvae had a tendency to clump or travel as a group; while the WT wandered and scattered individually. At the pupal stage the MUT Drosophila melanogaster had a uniform shape and size. All of the MUTs seemed to have spiracles. The WT pupa differed in size and shape, and spiracles were not noticeable in all of them. In the adult stage MUT wings were almost parallel to the body and close together, compared to the flared out WT wings. The antennae on MUTs also seemed to be larger than in WT. The mouth was also a noticeable difference, MUTs had a much smaller mouth than WT did. The most noticeable difference is the eye color and shape. MUTs had a
METHODS: In this experiment, the instructor provided us with 30 ebony individuals and 20 wild type individuals. In order to get an exact amount of each type, we anesthetized the flies and counted them off by gently using a fine point paint brush. Then all 50 Drosophila were put into a population cage which had a lid that had six holes for the centrifuge tubes. Two food tubes and four clean, empty tubes were added on the first day. Each food tube consisted of half a cup full of food mixed with 6-7 milliliters of water. This was the fly medium. The food should turn blue once the water is added. Each tube was labeled with a number and with the date. Every two to three days we added one more food tube until all 6 tubes contained the fly medium. After all 6 tubes were filled, the following days after we exchanged the first food tube with a new food tube. At the end of the experiment, we fed the flies with a total of 8 food tubes. Then the flies were anesthetized, again. At the end of this four week lab, the number of living ebony and wild
In order to obtain F1 data, monohybrid crosses were done, crossing virgin females for each of the traits mentioned with wild type males. 4-5 flies of each gender were anesthetized, and then put into a vial together, including media and a foam stopper, to mate. Anesthetization was done using FlyNap. One brush was dipped into FlyNap and inserted into the vial until 90% of flies were asleep. Media was created by mixing one small cup of potato flakes with a small cup of distilled water and 3 yeast pellets until it reaches a thick consistency.
Describe the sex and phenotype of the mutant fly. Describe the phenotype as it compares to the wild type.
4.14. The accuracy of the data is dependent on the correct controls being in place, Ensuring temperate stays the same throughout all solutions, making sure that the solutions are correct that the weight and size of the eggs are the measured correctly and that when the eggs are removed they are removed at the same time. Some of the eggs were not completely submerged in the solution this may cause some errors in the experiment and may have caused varying results. The eggs were of different sizes this also will cause the results to vary, Gathering eggs of exact size and weight would have ensured the results were correct. The eggs may not have been dried enough also causing results to vary; this can be fixed by ensuring that the eggs are thoroughly dried. The eggs were taken out at a time around about 24 hours, having an exact time to remove the eggs would have eliminated errors.
Running hot water over the metal lid of a glass jar makes it easier to open the jar. Why? Discuss two other practical examples based on matter and/or heat concepts.
Week 4: The F2 generation flies were present and identified by phenotype and gender. After identification, they were let go and the number of flies with each set of 2 traits were combined.
Drosophila melanogaster is a small, common fly found near unripe and rotted fruit. It has been in use for over a century to study genetics. Thomas Hunt Morgan was the best biologist studying Drosophila early in the 1900’s. Morgan was the first to discover sex-linkage and genetic recombination, which placed the small fly in the forefront of genetic research. Scientists have used Drosophila for many reasons. For one they are very easy to maintain, breed, anesthetize, and kill with little equipment. They are also very small and it is easy to distinguish males vs females and sexually mature flies and virgins. At lastly, the flies have a very short two week life span. On days 2-7 of their life
Hypothesis: If the temperature increases, then rate of the chemical reaction will increase as well, and if the temperature decreases, then the rate of reaction will also decrease.
Drosophila melanogaster show an innate behaviour where move towards a light source after being startled, showing positive phototaxis. This preference for light appears to be species- and strain-specific, and has been described as part of a fly's personality. Mated female flies have been seen to avoid UV light during egg laying. Wing defects have also been shown to affect phototaxis in walking flies as seen in flies with clipped wings who did not display the phototactic response to light. Manipulation of the wings which is a relatively unrelated organ and its effect on phototaxis suggest that it may not be a simple matter of stimulus and rigid, innate response, but that it contains at least a certain element of flexibility (Gorostiza, Colomb,
The short life cycle of the Drosophila Melanogaster consists of four stages. Drosophila start off as an egg and quickly hatch into a larva (Flagg 8). During the larval stage, the immature fruit fly is constantly eating in order to consume enough nutrients to undergo its metamorphosis from pupa to adult. As the larva gets ready to pupate, its outer covering becomes hardened and darker in color. In this pupal case, its wormlike body will undergo metamorphosis. After about two days, the new adult emerges and looks for a mate to start the cycle again. Males can be distinguished from females from simple observations of the abdomen. Male Drosophila are generally smaller than the
“Drosophila larvae are small, white, and glossy with a similar appearance to worms” (YourGenome.org, 2015). A female can lay 30-50 eggs throughout her lifetime at room temperature (YourGenome.com, 2015). Egg production has the possibility of becoming decreased at lower temperatures
In most kitchens the small flies that are found are Drosophila Melanogaster also called fruit fly. They are often brought in by ripened tomatoes, grapes and other perishable items from the garden. Drosophila melanogaster is a little two winged insect about 3mm long two winged insect that belongs to the Diptera, the order of the flies. The drosophila egg is about half a millimeter long. Fertilization takes about one day the embryo to develop and hatch into a worm-like larva. The larva eats and grows continuously, after two days as a third in star larva; it moults one more time to form an immobile pupa. Over the next four days, the body is completely remodeled to give the adult winged form, which then hatches from the pupal
Mature flies have no functioning mouth-parts and are not associated with transmission of diseases or considered pests
• medium heat : This starts from melting of Gandhak followed by profuse fuming. At this stage, clearing of the mouth of glass bottle by Tapta Shalaka (hot rod) is done. The temperature ranges between 350°C - 450°C.