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Separation Of Milk Lab

Decent Essays

Objectives: The point of this lab was to identify the major components of milk. It was also used to understand polar and non-polar properties to make a separation diagram. Introduction: The main purpose of this lab is to identify and separate the main components of milk. To do this an understanding of the properties of these components in needed to separate them from one another. We will be separating the components with their polarity or non-polarity and the temperature at which specific components precipitate. To do this we will be using hot plates, gravity filters and vacuum filters1, water and ice baths, and blot drying. Experimental Procedure: 100 mL of whole milk was measured in 250 mL flask and put on a hot plate in the hood. A thermostat was used to make sure that the milk did not go over 40 ͦC. When the Milk reached 39.2 ͦC, 11.0 mL of 10% acetate acid solution was added and stirred form 1 minute. Three coffee filters were nested into one another and used with a funnel to filter the solution into a clean 250 mL flask. A vacuum filter was set up while the solution was …show more content…

Each bubble of casein has a light negative charge. Negative charges are repelled so the bubbles of casein do not bump into one another. Because acid was added the milk gains a small electrical charge. When small amounts of positive charge are floating in the milk, the bubbles no longer repel so they bump together and stick. Instead of microscopic bubbles of protein we have the curds. The reason that the fat that was extracted sat on top of the water is because water is polar and the fat is …show more content…

The results obtained matched what was expected when the experiment was started. The procedure was successful in the fact that the components of the milk were separated and the qualitative tests went

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