Reaction Paper On Campylobacter

My unknown organism #11 is Escherichia coli (E. coli). E. coli belongs to the family Enterobacteriaceae. E. coli is Gram-negative, facultative anaerobe, and rod-shaped bacteria (bacillus). [1]

Bacteria are ubiquitous; they can be found on the skin, in the soil, and inside the body. Because of the very nature of this ubiquity, it is important to be able to determine between different strains of bacteria. An example of this is determining the causative agent for a disease so that the patient will be treated with the appropriate antibiotics. It may be important to determine the bacteria in a certain region, because like with enteric bacteria, it is normal to find them in the digestive tract as they are in a symbiotic relationship with our bodies in this area; however, they also cause opportunistic infections in places outside of the digestive tract to our detriment, such as with a urinary tract infection. Some strains of bacteria are common to nosocomial infections, and identifying these bacteria as such helps create the guidelines for healthcare workers in antiseptic technique. All of the morphology and characteristics of each strain of bacteria help us to better understand the role of bacteria in the body as well as helps us understand how they can cause illness, and what treatment regimen to set in place. In lab this semester, a sample of unknown

The identification of unknown organisms carries important ramifications that can be applied to many real world scenarios. In keeping with quality assurance beverages, food, cosmetics, and other products are frequently inspected for contaminants resulting from a presence of pathogenic bacteria. In medicine, a physician’s diagnosis and consequent treatment is largely determined from samples collected from infection sites that have been analyzed using microbial tests.

I'm John, and I helped to discover, harvest, and study some of the most promising bacteria specimens found recently. Although I cannot accept all the credit for the research, it was the entire crew's effort.

Bacteria are single celled organisms. Some classify them as a separate (fourth) kingdom on the tree of life.

Unknown lab report# 24 Introduction Microbiology is a study of organisms that surrounds us every day. It requires an educational understanding to identify organisms, and the ability to distinguish different bacteria’s. In applying the learning process of the different bacteria’s, unknown bacteria samples are given to be studied and identified.

During the final experiment for Microbiology will have to figure out the unknown organism given to us. The organisms will be narrowed down by using skills we have learned throughout the lab this quarter and the test results will narrow down to a single organism. The reason to perform experiments that determines the identity of bacterium is to find out how to treat it or what infections it is causing. In a controlled environment, students learned how to use various methods to determine how to differentiate between multiple bacteria. This lab report will be detailed steps explaining how to determine the identity of my unknown organism number 12.

2. List what it is caused by (specific scientific and common names of the organism: Escherichia coli

I inoculated a T-Soy agar with bacteria number 118, for this I used a streak isolation method. Next, in order to distinguish between Gram positive and Gram negative I used a streak isolation technique on a CNA plate, then performed the same exact procedure on a MacConkey plate. The results from the CNA plate showed the Gram Positive bacteria was an Alpha hemolyzer. Next, I used a P Disc on a T-Soy agar inoculated with bacteria 118 and determined the Gram Positive bacteria was not sensitive to P Disc antibiotics. This revealed the Gram Positive bacteria to be Streptococcus Mitis. The results from the MacConkey plate proved the Gram Negative bacteria to be a lactose fermenter. With the Gram Negative bacteria I performed a lysine test with positive results. Next, I performed an ornithine test on the Gram Negative bacteria, with negative results, therefore I concluded the Gram Negative bacteria was Klebsiella pneumoniae.

Campylobacter enteritis is frequent in young children (<4 years of age) and in adults (15 – 44 years) (Ftizgerald and Nachamkin, 2011).

Unknown bacteria determined to be Alcaligenes faecalis because of its morphological, physiological and metabolic properties.

Campylobacter jejuni is a gram-negative spiral pathogen. Most commonly, it can be found in chicken guts or animal feces due its nature to thrive in environments with reduced levels of oxygen. According to the World Health Organization in 2013, the said bacteria is the leading cause of foodborne diseases in the United States. This is most often times due to the consumption of undercooked poultry, most especially chicken. It is known that an infection called Campylobacteriosis, as well as the dreaded gastroenteritis, can be contracted from being exposed to C. jejuni, and it commonly brings about a range of food poisoning symptoms like diarrhea, vomiting, and dysentery syndrome.

It is believed that the first report about Campylobacter was back in 1886 by Escherich who observed and described non-culturable corkscrew-shaped bacteria isolated from the colonic contents of infants who had died as a result of “cholera infantum” (Escherich, 1886; Skirrow and Butzler, 2000; King and Adams, 2008; Vandamme et al., 2010). Campylobacter identified for the first time in 1906 by John McFadyean and Stewart Stockman, who reported the presence of large numbers of a peculiar organism in the uterine mucus of a pregnant sheep (Skirrow, 2006; Zilbauer et al., 2008). After this in 1913, McFadyean and Stockman isolate these microorganisms from aborted bovine fetuses and successfully cultured for the first time (McFadyean and Stockman,

Infectious diarrhea is the second cause of morbidity and mortality in the world. In addition, it leads to other serious long-term complications such as hemolytic uremic syndrome with renal failure that followed by enterohemorrhagic E. coli infection. Also, Guillain-Barré syndrome comes after C. jejuni

Common illnesses that can occur are salmonella and trichinosis poisoning resulting from improper handling of chicken, eggs or pork (Jardin, 2009, para.2). Diseases such as: campylobacteriosis, cholera, and listeriosis also make the list of illnesses (Jardin, 2009, para.1). These diseases are the versions of food poisoning, which can be life-threatening. According to the World of Health article published in 2007,