Purpose Of Osmosis Lab

The concentration of dissolved substances is greater in the extracellular fluid than in the cytoplasm. Hypertonic Solution Concentration of dissolved substances (solute) Concentration of water (solvent) What happens to an animal cells? Inside the cell Less Greater Outside the cell Greater Less Membrane

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Which of the following is true of osmosis? You correctly answered: c. It is a type of diffusion. 2. Which of the following occurs when a hypertonic solution is added to cells? You correctly answered: d. The cells shrink. 3. The variable that affects osmotic pressure is You correctly answered: a. the concentration of nondiffusing solutes. 4. The net movement of water would be into the cell in a You correctly answered: b. hypotonic solution.

Explain what happened to the blood cells at the various levels of concentration. Be sure to refer to the solutions as being hypotonic, hypertonic and isotonic.

The Osmosis and Diffusion lab was conducted to provide us with information on how built up mucus affects those conflicted by the recessive genetic disease, Cystic Fibrosis., due to a mutation to the membrane regulating chloride (Cl-). This mutation prevents the Cl- from leaving the cell causing the amount of sodium (Na+) in epithelial cells, which results in extreme mucus on the lungs and airways causing this disease to be fatal if not treated but treatment does not equate to a long lifetime. During the lab we took the data from three parts: Diffusion, Osmosis in an Elodea Cell, and finally the Role of Osmosis in Cystic Fibrosis. During Part 1 we looked at diffusion across a semipermeable membrane for starch and glucose, which resulted in both having a negative solution when placed in a semipermeable membrane. Then we looked at osmosis in the Elodea Cell to watch for the occurrence of Plasmolysis, when a cell’s plasma membrane pulls away from the cell, and how a plant cell is affected by both hypertonic and hypotonic solutions. Finally, we observed the role of Osmosis in Cystic Fibrosis using dialysis bags to represent a normal cell and a Cystic Fibrosis cell with the normal containing 1% NaCl while the Cystic Fibrosis bag contained 10% NaCl. After we ran the experiment, we looked at the Percent Change in Mass and compared them after 30 minutes. We found that Cystic Fibrosis cells didn’t change mass as much as the normal cell ending with a change in mass over -1%. The

In this lab experiment, half our group observed and measured osmosis using dialysis tubes that were represented as the semipermeable membrane. It is permeable to water and other small molecules but is impermeable to larger molecules such as the sucrose solution used in each of the four beakers and tubing. The other half of our group observed the tonicity of sheep blood to determine whether the blood was isotonic, hypotonic, or hypertonic. The 85 g/dL of NaCl solution was the ideal isotonic number in relation to the sheep blood cells as well as a reference to the other observations of the solutions.

The purpose of this experiment was to determine the effects of tonicity on a cell membrane using red blood cells, potato strips and three unknown solutions (A, B, C). First three slides were prepared containing RBC’s and unknown solutions A, B and C. A control slide was prepared only using RBC’s. After observing each slide under the microscope it was determined that unknown solution A was hypertonic because the RBC appeared to have shrunk. The RBC in unknown solution B appeared to be swollen, therefor, the tonicity of unknown solution B was hypotonic. Unknown solution C showed no change to the RBC shape, it was suggested that unknown solution C was isotonic. To confirm the tonicity

Osmosis is described in one of three ways when comparing more than one solution. The cell’s external and internal environment helps determine tonicity, which is defined as how the cell reacts to its environment. When the cell’s environment is equal in osmolarity to itself and there is no change, it is considered an isotonic solution. When the environment has a higher osmolarity, shrinkage occurs and it is considered a hypertonic solution. When the environment has a lower osmolarity, swellings occurs and it is considered hypotonic.

The lab for this paper was conducted for the topic of osmosis, the movement of water from high to low concentration. Five artificial cells were created, each being filled with different concentrated solutions of sucrose. These artificial cells were placed in hypertonic, hypotonic, or isotonic solutions for a period of 90 min. Over time, the rate of osmosis was measured by calculating the weight of each artificial cell on given intervals (every 10 minutes). The resulting weights were recorded and the data was graphed. We then could draw conclusions on the lab.

Osmosis is a special type of diffusion where water molecules move down a concentration gradient across a cell membrane. The solute (dissolved substance) concentration affects the rate of osmosis causing it either to speed the process up or slow it down. Based on this, how does different concentrations of sucrose affect the rate of osmosis? If sucrose concentration increases in the selectivity-permeable baggies, then the rate of osmosis will increase.

Osmosis is a special type of diffusion. It is the diffusion of water across a semipermeable membrane which is a membrane that is freely permeable to water but is not freely permeable to solutes, the water moves from a dilute solution to a more concentrated solution (Karp, 2010). Both diffusion and osmosis are passive transport, energy is not used in the transport. In osmosis water moves across a membrane toward the solution of greater concentration, because the concentration of water is lower there (Martini and Bartholomew., 2007).

Osmosis is a process in which molecules in a solvent pass across a semipermeable membrane into a more concentrated solution from a less concentrated one, attempting to make both sides isotonic or equal to each other. Isotonic can also be described as an equilibrium, where there is no net movement of the molecules. Osmosis is relevant in everyday life whether the general population is aware of it or not. It could be as simple as sitting in the pool too long and getting pruney fingers or as complex as a cholera infection in the intestinal cells that does not allow the intestinal cells

Osmosis is a natural occurrence constantly happening within the cells of all living things. For osmosis to occur, water molecules must move across a semipermeable membrane from an area of low concentration to an are of high concentration. In order to understand osmosis, people must understand the different types of concentrations that can be present within solution. One of them is an Isotonic solution where the concentration of dissolved particles is equal to that of a cell’s. Another is a hypertonic solution where there is a higher concentration of dissolved particles then inside the cell. And lastly there is a hypotonic solution where there are less dissolved particles than inside the cell. As dissolved particles move to a region of lower concentration, water moves the opposite direction as a result of there being less water in the highly concentrated region. In this experiment, gummy bears were placed in salt water, sugar water, and tap water to find the measure of osmosis between the solution and gummy bear.

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

The difference is that along with large molecules, living cells prevent molecules with positive charges and solubility. This is not representing in dialysis tubing, and is only found in living cells because the tubing is only based on molecular size (98). When referring the rate of diffusion, the concentration gradient influences the diffusion rate, based on the factors of temperature. The ability for molecules diffuse from high to low concentrations primarily depends on the concentration gradient between the two areas.(96-99). My hypothesis for the study is that in the hypotonic, hypertonic, and isotonic solutions, the direction and rate of osmosis will determine based on the concentration inside the dialysis tubing. My prediction is that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant.

When RBCs are suspended in an isotonic solution, nothing should be observed as there will be no net water movement between the RBCs and the solution. Thus, when 200 µL of blood was added to 10mL isotonic saline (0,154 M NaCl), voltage of 0V is recorded. When RBCs are suspended in a hypertonic solution, for instance, 0.4 M NaCl, RBCs shrunk due to decrease in cell volume as water diffused out of the cells by osmosis. The protein concentration within the cells become greater and more light is scattered and a negative