Microorganisms Vs Antiseptic Research Paper

AIM – The aim of the experiment is to determine the relative effectiveness of several anti-microbial substances on developing pathogens. (E. coli)

Before bathing/showering with soap and water prior to a procedure/surgery was accepted. It has been proven that antiseptic showering decreases skin microorganism count. Chlorhexidine gluconate products necessitate the need for several applications for the maximum antimicrobial benefit. Thus, each patient receives two preoperative antiseptic showers.

Rubbing alcohol, also known as isopropyl alcohol “refers to two water-soluble chemical compounds—ethyl alcohol and isopropyl alcohol—that have generally underrated germicidal characteristics” (“Guideline for Disinfection and Sterilization in Healthcare Facilities”). Ethyl alcohol kills bacteria in two ways: “protein denaturation and dissolving the lipid membrane” (“How Does Ethyl Alcohol Kill Bacteria?“). Next, Betadine, also known as Povidone-iodine is a solution [that is] often used in hand scrubs and hand rubs in hospital settings” (Ngan). It is used in “Treating minor wounds and infections, as well as killing bacteria” (“Povidone/ iodine solution”). Povidone-iodine’s way of killing bacteria is still being researched and “is yet to be fully understood, [but] it appears to have the ability to penetrate the cell wall of bacteria and affect their structure and function. This ensures their rapid destruction and helps protect against wound and skin infection” (“What is an Antiseptic”). Germ-X Hand Sanitizer is a hand gel that is used for waterless “washing” of hands to kill germs on the surface. It is an antiseptic primarily used by those who want to kill germs quickly and cannot wash their hands (Samuels). The formula contains about 63 percent ethyl alcohol according to livestrong.com (Samuels). Neosporin is a topical antibiotic used to

9. After the 48 hour time period the inhibition of the Escherichia coli bacteria around the disinfectant disks was measured. A ruler was used to measure the clearing from the edge of the disk to the perimeter of the clearing. Irregularities in the clearing were not included in the area of clearing.

When reflecting back to experiment 3, Aseptic Technique and Culturing Microbes, I realized the large amount of microorganisms that can be found in everyday life. Many different types are found with in the human body. Theses experiments focused on two types of bacteria. First was Staphylococcus epidermidis, found on the skin, and second was Lactobacillus acidophilus, found in the gastrointestinal tract. Both have similar needs for growth when it comes to temperature, however, different growth environments are used.

1 and 3). This antibiotic is effective against both gram positive and negative bacteria, so it would be effective against Staphylococcus epidermidis. This antibiotic is able to kill bacteria by interfering with the cell wall synthesis, weakening it, and causing its death. However, just like with Penicillin, some bacteria have become resistant to this antibiotic (6). The hypothesis stated that Staphylococcus epidermidis would be susceptible to Ampicillin because although some bacteria have become resistant this one has not (7). According to the class data, two of the tests resulted in in susceptibility, one resulted in an intermediate effect, and the rest were resistant (see Figs. 2 and 5). It seems that Staphylococcus epidermidis is in the process of becoming resistant to Ampicillin because of the varied data. However, the group data did not support the hypothesis that the bacteria would still be

Research Question: What is the antimicrobial effect of common household spices/herbs such as cinnamon, cloves, mustard, ginger, and garlic, on the zone of inhibition in the Staphylococcus epidermis covered nutrient agar?

Out of the all the antiseptics being used in this investigation the more well known antiseptics such as dettol and sudocrem will be more effective when stopping the growth of bacteria than natural antiseptics like lemon.

The zone of inhibition, the circular area lacking bacterial growth around the test disk, was used to study the effectiveness of the antibacterial solution. The compounds used in the experiment were 10% solutions of Febreze, Clorox, Anti-bacterial soap, moisturizing soap, Bactine, and Hydrogen peroxide.

When analyzing all of the data in the graphs and the t-test charts, the researcher was able to come to the conclusion that the data supported his hypothesis that the oleuropein created the largest kill zone area. Not only was there a visible difference of oleuropein’s kill zone of the K-12 E-coli bacteria as seen in graph 1.1, but there was a statistically proven significant difference (tables 2.2, 4.1, 4.2, and 4.3). The data collected by the researcher supports the current research that oleuropein is a natural antiseptic. That oleuropein has very strong antibacterial properties that can be used for medicinal use (Omar, 2010).

5) My hypothesis: “The higher the concentration of antiseptic, the more powerful it will be at inhibiting/ killing microorganisms which cause bacterial growth.” This theory supports my result because as you can see the zone of inhibition increases due to the increasing concentration of antiseptic. The higher concentration of antiseptic means it has been less diluted, which means its more stronger than other more diluted concentrations, this high concentration will determine how powerful the antiseptic will be at killing/inhibiting the growth of bacterium, this high concentration of antiseptic will break down the bacterial cell wall, after this process has happened the antiseptic can stop the mutation of the bacteria and inhibit the

In the future, any further investigations into the effect of antimicrobial substances may wish to investigate the effect of different leading brands of antiseptic solutions that contain the active ingredient Chloroxylenol, and how they affect the growth of bacteria as well as, whether different ingredients, cause the solution to be more or less effective in inhibiting bacterial growth. This will allow consumers and medical professionals to determine which brand or type of antiseptic solution is the most effective in protecting against pathogens such as

This assignment will encompass how the results of the personal hygiene and susceptibility microbiology experiments provide a framework for the basis of the NICE Clinical Guidance (CG139) on Infection and how crucial the reasons for the hand wash protocol and hygiene is in all healthcare settings.

The very process of reviewing the literature resulted in the scope of the topic narrowing from “antibiotic use in livestock” down to “thyme oil as an alternative for one particular livestock disease”. This review is organized in chronological fashion, reflecting the literature search process through time as new information informed closer, more specific focus.

Antiseptics are antimicrobial substances applied to skin, mucous membranes or wounds for their bactericidal or bacteriastatic anti-infective effects (McDonnell and Russell, 1999).