Major Steps in Plant Tissue Culture Essay examples

8. I added the 3 ml of soap to the beaker and stirred the solution 40-50 times with a fresh pipette.

Wait, another 3 minutes. While waiting for Plant A to soak, proceed to put Plant B (the leaf exposed to no air through soda lime) into the same beaker of boiling water. Once time is up for Plant A, rinse the leaf with water and place leaf off to the side. Next, grab tongs and place Plant B directly into the beaker with alcohol solution (Making sure to grab new alcohol into the beaker and not using the same solution from Plant A). Wait, another 3- 5 minutes. Once time is up place Plant B, using tongs, into the petri dish and completely cover the leaf in iodine solution. Wait 3 minutes. Then rinse off the leaf and compare the coloration with Plant A. Record

There are many procedures during this lab and many materials needed for an accurate analysis of data. First, fill a 100 mL graduated cylinder with 50 mL of water. Add 25 germinating peas and determine the amount of water that is displaced. Record this volume of the 25 germinating peas, then remove the peas and put those peas on a paper towel. They will be used for the first respirometer. Next, refill the graduated cylinder with 50 mL of water and add 25 non-germinating peas to it. Add glass beads to the graduated cylinder until the volume is the same to that of germinating peas. Remove the beads and peas and put on a paper towel. They will be used in respirometer 2. Now, the graduated cylinder was filled once again, determine how many glass beads will be require to reach the same volume of the germinating peas. Remove the beads and they will be used in respirometer 3. Then repeat the procedures used above to prepare a second set of germinating peas, dry peas and beads, and beads to be used in respirometers 4,5,and 6, the only difference is the temperature of the water.

14. Use the same loop and technique to spread the same cell suspension (+) on the LB+AMP agar plates. Dispose of the sterile loop in a beaker of germicide.

Please answer these questions then place them in the drop box for this lab. Use Microsoft word if possible.

Next, aseptically add 20mL of T-soy broth and 2mL of late log phase of B. thuringiensis culture. Incubate this flask for 24 hours at thirty degrees Celsius shaking at 180rpm. The next time in lab, remove between 1 and 1.5 mL from the top of the tube that holds the soil using a syringe. Open a package of .22μm filter and place the syringe in the top of the filter and dispense the liquid into a centrifuge tube that is labeled as 100 and close the tube immediately. For the negative control, dispense 1mL of SM buffer into a microcentrifuge tube labeled negative control using a syringe. With four microcentrifuge tubes label them -1, -2, -3, and -4. Add 90 μL of SM to each tube, and then add 10μL of the 100 tube to the -1 tube and vortex. Then add 10μL of the -1 tube to the -2 tube and vortex. Keep this process going till the -4 tube. Next, dispense 50μL of the undiluted sample into .5 mL of B. thuringiensis and then vortex. Now mix in 5 mL of TA to the culture tube and pour onto a plate labeled 100 and let solidify. On a different plate, draw a grid with sections labeled negative control, -1, -2, -3, and -4. Aseptically add 4.5mL of TA with .5mL of B. thuringiensis by pouring and then pour onto the plate evenly. Let the plate sit for about ten minutes. After ten minutes transfer 5 μL of the negative control and the dilutions into the proper

There were two tubes used in this process: the tube that contained the primary culture and the tube that contained the nutrient agar where the unknown bacteria would grow. First, the inoculating loop was flamed. After removing the caps of both the test tubes, they were flamed to prevent contamination of the unknown bacteria. The inoculating loop was cooled for a few seconds and was then placed into the test tube containing the bacteria. The inoculating loop with the bacteria was placed into the nutrient agar test tube for cultivation. Before the test tubes were capped, they were flamed once again. Also, isolation of the unknown bacteria had to completed. Nutrient agar was placed in the petri dish, and was left to gel for a few minutes. After the agar gelled, the inoculating loop was used to acquire bacteria and streak the unknown onto the plate for

From a diagram of an idealized flower, correctly label the following structures and describe the function of each structure:

Add three seeds to the potting mix and cover seeds with little remaining potting mix. After the addition of the potting mix, use a dropper filled with water and water each cell until water drips from the wick. Then place the quads on a watering tray under the fluorescent light bank. Each cell should have an equal distance from the light bank. Quads should be three inches below the fluorescent light; the light should also be left on all day. Make sure all wicks are in contact with the mat that sits on the watering tray. Also watch out for the watering system regularly throughout the experiment. After four to five days record plants in the quads, giving their phenotypes in a table for each cell removed all but the strongest plant.

The solution has been dechlorinated and adjusted to be slightly acidic. Place 75 mL of the solution in each of three labeled beakers. Obtain an animal organism, small fish, and a plant organism, Elodea. One beaker will be the control and will not have anything in it. Place exactly 25 mL of water in a 50-mL graduated cylinder. Place each organism in a cylinder and note the increase in volume above the original 25mL. The increase equals the volume of the organism. After taking measurement, cover each beaker with the plastic film. Place the beaker containing the Elodea in the dark by covering it with aluminum foil. Allow organisms to respire for 15 min. Gently remove the organisms from the beakers and return them to their original culture bowls. Then add four drops of phenolphthalein to the contents of each beaker. The solutions should remain clear because the solutions are acidic. Using a dropper bottle, dispense NaOH into the contents of the beaker drop by drop. Thoroughly mix the contents of the beaker after adding each drop. Continue adding drops until you first notice that the solution turns pink. Repeat for each beaker with at the living organism until the solution is the same shade of pink as the

The purpose of the “chi-square test” was to see if our data was in an acceptable range of a specific ratio listed above. The chi-square test took into account the expected deviations in the F2 offspring’s alleles.

Before proper tests can be performed, the most important aspect to laboratory procedures are aseptic techniques. These techniques are crucial to ensure that no contamination occurs during the culturing process (1). Although the exact steps may vary depending on the media and type of transfer, the main components of the aseptic techniques

Embryonic stem cell research is a controversial topic nationwide, because of its clash of ethical and moral values. Many people, including those suffering from diseases that this research is seeking to cure, do not believe in killing a living embryo in order to advance research and science.

Read in your lab manual about the following agar mediums: Blood Agar (pg 168), EMB Agar (pg 170), Mannitol Salt Agar (MSA)(pg 172) ), MacConkey Agar (pg 174), and PEA Agar (pg 176) to answer the following:

The tobacco plant like many plants contain a cell callus. A cell callus contains somatic undifferentiated cells and can be used to differentiate into specialized tissues of the tobacco plant, or any plant used, by being induced with the addition of different types of hormones, such as cytokinin and auxin. Cytokinin and auxin are mostly used in plant tissue culture simultaneously to provoke the formation of a plantlet or callus. There is a common use of Kinetin in plant tissue culture since when added it will promote cell division to initiate shoot tissues from calluses of the plant. Kinetin is a type of cytokinin hormone. In regards to auxin related hormones, Indole-3-acetic acid (IAA) is also commonly used since it promotes the initiation of roots for the root tissue of the plant. In this