Lab And Using The Result Of The Chemiluminescence Reaction

294). This means even after a week the blood has been cleaned up, luminol can still detect it. Also after applying luminol, the area needs to be darkened so that blue glow can be emitted. “The iron from the hemoglobin in the blood causes luminol to glow, so a blue glow is produced when the solution is sprayed where there is blood. Only a tiny amount of iron is required to catalyze the reaction” (Helmenstine, 2012). The use of luminol revealed that there were blood stains on the bed sheets and suspected that the woman was killed in her bedroom and that she did not go to work that day or leave the house.

In this experiment, luminol, a compound that produces chemiluminescence when oxidized in the presence of a base by an oxidizing agent like hydrogen peroxide, was synthesized, and its presence was confirmed by observing chemiluminescence. Cheminluminescence is the process by which a photon is released when a reaction occurs, producing light. A similar process, bioluminescence is observed in nature when fireflies and jellyfish produce light. Chemiluminescence is identical to bioluminescence, except for the fact that bioluminescence involves an enzyme, whereas chemiluminescence uses a chemical catalyst.

Type of Reactions We did a lab on many reactions to find the products of the reactions. We conducted four group experiments and three class experiments, following very strict safety procedures given to us by our instructor. We used goggles and aprons which were required at all times. We could not sniff any gasses or walk into clouds of smoke unless we were instructed to.

This experiment is about glow sticks so here are some facts. Let’s talk about glowsticks and everything about them. Light is generated from a chemical reaction which is called chemiluminescence. Typical glow sticks use a hydrogen peroxide solution, a solution of phenyl oxalate ester, and a fluorescent dye. Glow sticks are plastic tubes containing glass vials that emit light when they are cracked/ bent. They have a glow time from 6-12 hours. It also depends on the size of the glow stick and other stuff like that. Glow sticks are also waterproof if you did not already know that. Energy created by the chemical reaction is what creates the color. If glow sticks did not have fluorescent dye there would be no color. Glow sticks might look like they

Everyone one has seen a crime scene show or at least a glimpse of one. If not well then you won't have a clue of what I’m talking about so let me enlighten you on the subject. My experiment has to do with crime scene investigation (C.S.I), and what they use to help catch the monster or perpetrator. The C.S.I investigators use this chemical solution that will light up a bluish green color when it comes to connecting with blood and many other bodily fluids. The history is really not that long because luminol

The main part of this reaction and also the way light is produced is when the iron in the blood oxidizes with luminol, resulting in an electronically charged particle, known as 3- amino phthalate, which then produces the blue light that we see (Micheal Seery).

In the past decades lasers have become evidently important to society. Lasers are normally perceived as devices used by our hands or large boxes found in research laboratories. Another evolvement in science that has taken place is the use of fluorescent dyes. Fluorescent dyes have become a common use for research and diagnostics in order to identify specific cell and tissue types. The use of such a dye is to identify for example, various chemical substances such as proteins, tag cells and DNA and their concentration. The only problem with such dyes is only as few as 10 colours have the ability to be distinguished. These newly developed lasers formed by fat cells are a combination of solid styrene beads. The purpose for this evolution is to capture light that is taken from the cells which are views using a spectrometer and thus, analyze the spectrum. It serves a function of measuring refractive index change. Before this step forward the ability to tag cells stood at around a couple hundred cells. Fat cells already consist of lipid droplets and act as natural lasers as it is. They need nothing more than to be supplied with nontoxic fluorescent dye. This new laser technology can help us further understand the cellular process and further improve medical diagnosis and therapies. Considering the level of

When we finished collect the data we make a graph. We start with white color light first trial. We saw 4-5 of Daphnia come stayed few second and two of them left. We get same result on second trial. When the third observation was different because only one Daphnia was left all of them went to dark place. While we are observing the Daphnia with red color light they are not attractive. Two of Daphnia came and stay about five second and one left only 1 was stayed stayed stayed We use the same light second time same result we got only 1 stayed. We use the same color light for third trial we saw some difference. They are was no Daphnia at all . None of Daphnia accept the red color. Finally we use green color. First trail was strange none of Daphnia came to near the light also same result in second trial. The third trial one Daphnia came on nearby the green color. Our group find out that white color light was most attractive for Daphnia. My dependent group is number of Daphnia attracted with light. My independent group is diffrent color of light. My control group is white light and my experimental group is green light and red light.

We’ve all seen the cop shows, however, what they neglect to show is the full extent of the work people like forensic analysts are responsible for behind the scenes. One of the uttermost important things they do is find out where the blood is at a crime scene, this can be difficult if the blood evidence has been cleaned. The primary way they find blood, even if it isn’t visible is by using luminol. One issue with using luminol is that the light doesn’t last very long (K. L. Lerner & B. W. Lerner (Eds.), 2006). Scientists, Katie Sosebee and Zenobia Merchant, are conducting experiments to test the effect of different temperature water to the longevity of luminols light.

Today I read an article on bioluminescence. Bioluminescence occurs when two types of chemicals come together to create a chemical reaction. Luciferin and either luciferase or photoprotein come together with oxygen it combines to form a different chemical that shows off the light. Bioluminescence can be found mostly in marine life, for example fish, bacteria, and jelly. It can be found on land in fireflies, fungi and railroad worm. Bioluminescence has been adapted to ade in many different task from mating, hunt prey, defending from predators and more! In the article it talked about how humans could use bioluminescence in everyday life as a safer alternative to light.

Luminol (5-Amino-2,3-dihydro-1,4-phthalazinedione) is a compound that made up with carbon, hydrogen, oxygen and nitrogen, the chemical formula of luminol is C8H7O3N3, it is in a form of powder at room temperature and room pressure. The chemical reaction of luminol and the reactant is a chemiluminescence reaction, which emits strong blue light during the reaction. Luminol can’t be used alone, in order to use it, criminalists must first activate it first. Luminol needs to mix with a strong oxidizing agent such as hydrogen peroxide (H2O2). Other than a strong oxidizing solution, a basic solution is also needed, for the basic solution, alkali like sodium hydroxide can be used. Addition of basic solution and strong oxidizing agent allow luminol molecules have

The experiment utilized acid-base reactions (Table 1) to manipulate the extraction and isolation of the three components. The first component that was extracted from the three component mixture was the base m-Nitroaniline. HCL was added to the three-component mixture, the base m-Nitroaniline separated from the ether, benzoic acid, and naphthalene. After this acidic extract was separated into an Erlenmeyer flask along with the water extract, the acidic extract was converted back to m-Nitroaniline by adding NaOH until the solution became alkaline. Following vacuum filtration the remaining compound was observed as yellow powder. Benzoic acid was then separated and extracted after adding NaOH. HCL was then added until the solution became acidic.

In the following experiment, fluorescence methods and techniques will be used to determine the detection limit and the concentration of quinine in tonic water. As shown in the graph of the quinine standard samples, the concentration was on average was 350 ppm. This concentration is incorrect, since it did not factor in the max emission and the emission at smaller concentrations. Despite the error in the calibration data, the pH data was able to be observed and assesse the relationship of pH to emission. As the pH increase the emission decreased, which showed that there was minimal concentration quenching. The detection limit of the fluorimeter was calculated using the data from the calibration. Meaning that there will be slight error within the calculations. It was determined to by approximately 3 ppm.

Blood is the most common type of biological fluid found at crime scenes. Blood consists of plasma, red blood cells, white blood cells, and platelets (Houck & Siegel, 2015, Chapter 10, p. 240). Red blood cells transport oxygen and carbon dioxide throughout the body and is accomplished by hemoglobin. That being said, when a presumptive test is done to test for blood, the presumptive test will react with the hemoglobin that is present in blood. One thing that might happen is the stain

Bioluminescence is the production of light by living organisms that involve biochemical reactions. Fluorescence is the emission of light through absorbing short wavelengths of light and emitting that light in longer wavelengths. Both processes emit light but do so in different ways. With bioluminescence, light energy is released by the biochemical reaction that happens inside the organism and does not require an external input of energy. In contrast, fluorescence does not involve biochemical reactions but instead requires an external source of light energy that is absorbed and then re-emitted as a longer wavelength.