Design: Investigation of Fermentation Introduction Introduction Yeast (Saccharomyces)is a single-celled microorganism in the Fungi family. It anaerobically respires sugars to produce ATP, as well as the waste products ethanol and carbon dioxide gas. This process is known as fermentation. There are various factors that affect the rate at which yeast respires. Aim To investigate the effect of concentration of table salt (sodium chloride) on the rate of fermentation of sucrose using yeast, measured in the volume of carbon dioxide gas collected over a period of 10 minutes. Research question What is the effect of concentration of table salt on rate of fermentation? Hypothesis I predict that as salt concentration is increased, the rate …show more content…
3. Weighing boat and scales were used to measure up 10 g of sugar, which was poured into flask “0%”. 4. Weighing boat and scales were used to measure up 10 g of yeast, which was added to flask with sugar. 5. Measuring cylinder was used to measure up 80 cm3 of tap water, which was added to flask containing sugar and yeast. 6. Contents of flask were stirred using stirring rod. 7. Flask and delivery tube with plug were set up in a water bath of 37-40°C with a clamp-stand (according to Fig. 1). 8. Measuring cylinder was filled completely with water and turned upside-down in bucket, over delivery tube (according to Fig. 1). 9. Stopwatch was started. 10. After 10 minutes, volume of gas collected in measuring cylinder was recorded. 11. Apparatus was rinsed. 12. Steps 2-3 were repeated using flask “1%”. 13. Measuring cylinder was used to measure up 79 cm3 of tap water, which was added to flask containing sugar and yeast. 14. Weighing boat and scales were used to measure up 1 g of table salt, which was added to flask containing sugar, yeast and water. 15. Steps 6-11 were repeated. 16. Steps 12-15 were repeated for the remaining salt concentrations (flask “5%” contained 5% salt, i.e. 5 g salt and 75 cm3 of water; flask “10%” contained 10% salt, i.e. 10 g salt and 70 cm3 of water, and so on). 17. Steps 1-15 were repeated five times. 18. Apparatus was cleaned
First students obtained 8 graduated cylinders and labeled them. Each one contained a different ratio of vinegar and water. These graduated cylinders with the liquid were weighed and recorded.Then, students obtained an Alka-Seltzer tablet and recorded its mass. Then one alka-seltzer tablet was dropped into each of the graduated cylinders. Students had to
I took the graduated cylinder and started filling it up with water until the bottom of the meniscus was to the the 100.0 mL mark with the assistance of a dropper pipet. I then took the 13 x 100 mm test tube and slowly poured the water from the graduated cylinder into the test tube until it was full to the top. I then poured the water in the test tube out into the sink and put the graduated cylinder on the counter so I can get an accurate measurement of the lower meniscus to record on my data table. I once again followed the same procedure again filling a second test tube with water from the graduated cylinder then setting it on a straight surface to get an accurate measure of the volume to
Procedure: Measure the volume, mass, length and temperature of a variety of items. Create dilution of sugar water.
The measuring cylinder was then used to accurately measure out 20 mL of water, which was then poured into the test tube that would be used for the experiment. The test tube was then placed into the clamp, which was then adjusted in order to make sure that the test tube was grasped firmly and would not fall out.
Procedure: I used a ruler, thermometer, and scale to take measurements. I used a graduated cylinder, short step pipet, scale, and ruler to determine volume and density. I used a volumetric flask, graduated pipet, pipet bulb, scale, and glass beaker to determine concentrations and densities of various dilutions.
The volume of a small test tube and a thin-stemmed pipet were determined in this section of the lab. Water was poured into a small test tube until the water reached the very top edge of the test tube. The test tube was then emptied into a plastic 25 mL graduated cylinder and volume was measured and recorded into data table 3. A think-stemmed pipet was completely filled with water. Drops were carefully counted and emptied into the empty plastic 25 mL graduated cylinder until the water level reached 1 mL. The number of drops in 1 mL was recorded into data table 3. The thin-stemmed pipet had a total volume of 4 mL and that was also recorded into data table 3.
Respiration is a chemical process by which organic compounds release energy. There are two types of respiration reactions that cells use to provide themselves with energy: aerobic and anaerobic (fermentation). (Chemistry for Biologists: Respiration. 2015) Both processes are similar within the initial steps of the reaction- beginning with glycolysis. However, in fermentation instead of the pyruvic acid being converted to acetyl coenzyme A, it’s converted into both ethanol and carbon dioxide in yeast and some plants and lactic acid in animal cells. Another distinct difference between the two processes is that anaerobic respiration uses oxygen
* By using the dropper and measuring cylinder, an amount of 5ml milk was placed in the test tube
0.1 gram of my product from the second trial was weighed in a tray and was then added to a fourth test tube containing 2.0 mL of Iron (III) chloride, which was measured using a 10 mL graduated cylinder, to test for
Rate of Respiration in Yeast Aim: I am going to investigate the rate of respiration of yeast cells in the presence of two different sugar solutions: glucose, sucrose. I will examine the two solutions seeing which one makes the yeast respire faster. I will be able to tell which sugar solution is faster at making the yeast respire by counting the number of bubbles passed through 20cm of water after the yeast and glucose solutions have been mixed. Prediction: I predict that the glucose solution will provide the yeast with a better medium by which it will produce a faster rate of respiration.
2. Fill a flask with pure water (0% sugar), another with 0.2M water, another flask with 0.4 M water, and another flask with 0.8M water.
The materials used during the experiment included three plastic cups, three gummy bears, masking tape, marker, balance, calculator, tray, one plastic spoon, a measurement tray, and a ruler. The three plastic cups were used to hold the tap water, salt water, and sugar water. The masking tape and marker were used to label each cup with the
This lab investigates the effects of Sucrose concentration on cell respiration in yeast. Yeast produces ethyl alcohol and CO2 as a byproduct of anaerobic cellular respiration, so we measured the rate of cellular respiration by the amount of CO2
5) 5 mL of sugar was added to the solution and a balloon was placed over the opening of the bottle to minimize the loss of any gas from the system.
Fermentation a metabolic process with occurs in the absence of oxygen molecules also known as an anabolic reaction. It is a process of glycolysis in which sugar molecules are used to create ATP. Fermentation has many forms the two most known examples are lactic acid and alcoholic fermentation (Cressy). Lactic acid fermentation is used in many ranges from food production such as bacteria to its use by fatigued muscles in complex organisms (Cressy). When experimenting with organisms such as yeast which was done in this experiment you follow the metabolic pathway of Alcoholic fermentation (Sadava). Where the sugar molecules are broken down and become ethanol (Sadava). But the end product of fermentation is the production of