Materials/Methods and Results-Haematology Practical
Haemoglobin Estimation: To determine the amount of haemoglobin concentration in the patient’s blood, an anticoagulated blood sample from the patient and a healthy control, were lysed by converting haemoglobin into cyanmethaemoglobin. Potassium ferricyanide and potassium cyanide are used for this conversion to occur, and the absorbance of this colouring was measured at 540nm using a spectrophotometer. The results were recorded for the patient and the control, with the haemoglobin concentration seen below.
Results for the patient and control samples
Sample Absorbance Readings 1 2 3 Mean
Reference preparation 0.378
Patient Sample 0.546 0.552 0.618 0.572
Control Sample 0.622 0.617 0.638 0.626
Figure 10
Results showing the haemoglobin concentration for the patient and the control Haemoglobin Concentration (g.L-1)
Patient Sample 174
Control Sample 190
Figure 11
Red Blood Cell Count:
Control Results for the number of red blood cells
Chamber Number of cells counted
1 72
2 116
3 86
4 99
5 81
Total number of cells in central square: 2230
To determine if there was an increase or decrease in red blood cells in the patient’s blood, a counting chamber was filled with a whole blood sample from the patient, which was diluted with an isotonic diluent. Using the 4mm objective lens, the central 1mm2 area of the grid was found, and in this grid, only the four small corner squares and the central square were counted for red blood cells. The
To test the blood, we first dropped two drops of blood into two spots in a micro-well plate, then we added the appropriate serum to both and mixed with a small stick. One side was labeled A, for the A Anti-Serum, and one was labeled B. If the A blood clotted it meant that the A antigen was found, and likewise with B. If both clotted the blood type was AB, and if neither clotted the blood was type O. Some errors made
Exercise 11: Blood Analysis: Activity 1: Hematocrit Determination Lab Report Pre-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. 1. Hematocrit Your answer : a. refers to the percentage of white blood cells (WBCs) in a sample of whole blood. Correct answer: b. of 40 means that 40% of the volume of blood consists of RBCs. 2. A buffy coat layer You correctly answered: d. is all of the above. 3. The diagnosis of anemia indicates You correctly answered: a. a lower-than-normal hematocrit. 4. Polycythemia refers to You correctly answered: c. a significant increase in RBCs.
1.Full blood counts: To assess the Hb for evidence of anemia, WBC for evidence of infection.
The goal of this experiment is to determine the blood types of the samples given and to learn what interactions occurred to each blood type. Determining an individual’s blood type and how it reacts with Anti A, Anti-B, and Anti Rh serums played a crucial part in this experiment. The researcher concluded that agglutination (clumping) occurred in some of the blood samples. For example, Mr. Smith’s blood reacted with Anti-A and Anti-Rh serums (antibodies) allowing the researcher to determine the blood type is A. Mr. Jones’s blood reacted with Anti-B serum but it did not react to Anti-A or Anti Rh allowing the researcher to believe that the blood type is B. Mr. Green’s blood reacted with all serums and caused a reaction to occur resulting the blood type to be AB positive. Mr. Green’s blood also had a positive marker for Rh factor. However, Ms. Brown’s blood had no reaction at all and the researcher determined if no reaction occurred then the sample had no antigens but proved to have some antibodies, resulting in blood type to be O. The purpose of this experiment is to determine whose blood has type A, B, AB, or O.
The purpose of this experiment was to determine the effects of tonicity on a cell membrane using red blood cells, potato strips and three unknown solutions (A, B, C). First three slides were prepared containing RBC’s and unknown solutions A, B and C. A control slide was prepared only using RBC’s. After observing each slide under the microscope it was determined that unknown solution A was hypertonic because the RBC appeared to have shrunk. The RBC in unknown solution B appeared to be swollen, therefor, the tonicity of unknown solution B was hypotonic. Unknown solution C showed no change to the RBC shape, it was suggested that unknown solution C was isotonic. To confirm the tonicity
There was a key on the dipstick container, and the colors of the squares were compared to the key. The information was then recorded in a results chart. At forty seconds, the ketone square was observed and recorded. At forty five seconds, the specific gravity number was determined and recorded. At one minute, the presence of red blood cells, albumin, nitrites, and urobilinogen was recorded based off the color and key of the dipstick.
The purpose of this lab being performed is for one to determine the normal osmolarity of our fluids, and how the different concentration solutes has an impact on the red blood cells shape. Depending on the solution being used the cell can either, swell up, crenate, burst, or keep its shape. Depending on the osmolarity of a cells interstitial fluid, the cell might change its tonicity if osmosis occurs. Osmolarity is the concentration of ions in a one-liter solution. We will be able to determine what solutions causes change in shape and tonicity by the different types of solutions we have by changing the extracellular fluid concentration of the solutes in the red blood cells. We will place the red blood cells in a 5% hypertonic solution, hypotonic
In the continued process of realizing how much sugar content was in the food and beverages I was consuming and going to the doctor receiving my blood work results, I decided to change my nutritional habits. I went to my primary care provider to get my six-month checkup and was informed that my Hemoglobin A1C levels were high (5.9%). Over the course of the summer/winter break I was consuming too much of sugary food items and beverages while on a cruise as well. I can agree that I consumed large amounts of sugary foods while on my summer break and now this has changed my way of thinking about nutritional sugary content. Whenever I would feel the need to have a snack, I would want something sweet to curve my appetite at the moment.
Intraoperative ultrasonography has been used to determine whether there is metastatic disease to other organs. Laboratory test will exhibit elevated WBC and AST. A complete blood count (CBC) is a common laboratory test used to measure blood counts (or the number of blood cells circulating in the bloodstream). A CBC measures the level of red blood cells (which carry oxygen throughout the body), white blood cells (which fight infection), and platelets (which help with blood clotting) in a blood sample. A CBC also measures hemoglobin (a protein in red blood cells that carries oxygen) and hematocrit (the ratio of red blood cells to
HOSPITAL COURSE: This 36-year-old gravid of four para 2012, now para 3013. Presented in labor and to delivery at 38 and 3 weeks at just gestational age with spontaneous ruptured membranes. During her labor course she was known to have fetal mouth presentation in the form of breech, and the decision was made to perform a primary cesarean section, secondary to this breech position. The risks, benefits, and alternate procedures were discussed and the patient expressed understanding. All questions were answered; patient's preoperative hemoglobin were 12.7. A primary low transverse cesarean was performed, please see separate operative report for
Anemia is a worldwide condition that affects many people of various ages, lifestyles and health history. This condition affects the red blood cells in the body when not enough hemoglobin is being made due most often to the lack of iron in the body. A way to help with this is by administering drugs, such as Epogen and Procrit, which can increase the hemoglobin level in the body. There is no one optimal hemoglobin level for everyone as it varies due to different factors, including sex, health, and lifestyle.
To begin this portion of the total blood cell count a finger prick was completed. In order to do this gloves were put on the participant’s hands and the finger being used to prick was thoroughly cleaned with an alcohol pad (allowed to air dry). Next a sterile lancet was used to stick the cleaned finger until blood appeared. Holding the hand down, two capillary tubes were filled at least two thirds of the way full with the participant’s blood. After the capillary tubes were filled they were sealed by pressing the clean end into the clay pad. Both capillary tubes were then placed onto the centrifuge for three minutes to spin, (which separated the elements of blood). The group then used a hematocrit card reader to measure the percentage of red blood cells. To do this the bottom of the capillary tube was placed on the scale at 0 and the top of the plasma at 100. The value at the top of the red blood cell portion indicates the percentage of whole red blood cells. Materials for collecting the white blood cell differential information included three clean glass slides, a sharp, wrights stain, a buffer for wrights stain, bibulous paper, a stacking rack, and a microscope. This experiment also requires a finger prick, if more blood was needed then it was performed again as previously stated. A small drop of blood was placed on two of the three clean slides. The third clean slide was used to smear the blood on each of the slides containing the drop. The slides were to be held flat while the third slide was used to push the blood down the slide in a thin layer. After a thin blood smear was created on both slides the blood was allowed to air dry for the staining process. Following the slides drying, they were placed on a stacking rack with the blood
While viewing the red blood cells, we were able to view
Investigating haemoglobin (Hb) concentration in blood samples using the haemoglobincyanide method and in foetal haemoglobin samples
Blood chemistry or blood test is identifying the different levels of chemical ingredients found in the blood. The analysis of these substances provides clues to a patient’s condition and the functioning of the major body systems such as, blood sugar levels, liver functions, essential nutrients etc. This helps doctors in diagnosing the patient’s conditions much easier and quicker.