Gas Chromatography Lab

After performing gas chromatography, the identity and the ratio of unknowns are determined by using retention time and areas. The retention time, which states how long each sample takes after injection to move through the column and interact with the detector, is used to distinguish the identity of the unknowns. Each compound has its own retention time. The peak area that combined underneath each peaks is used to determine the ratio of the unknowns. In this lab’s gas chromatogram, there were three

to understand how the percentages of each of the compound is determined. It is mainly possible due to the idea that gas chromatography is utilized. That being said, the equation used in the last lab report is again going to be used here because of the fact that the gas chromatography was used again to get the integrals of the graphs (Lab 2). The equation for that is as follows (Lab 2): (Area of Peak 1)/(Area of Peak 1+Area of Peak 2) x 100 Thus, it is important to note that if this equation was

Gas-liquid chromatography (GLC) is a process where an unknown organic sample is dissolved in a solvent then vaporised to separate it into its’ components. This is carried out by using two phases; the stationary phase and the mobile phase. The mobile phase is the gas containing the sample and the stationary phase is a liquid absorbed in a solid support. The liquid can be changed depending on the mixture being tested, so the stationary phase is packed in to a long, thin tube called the column (4college

Introduction: Using the analytical methods of Gas Chromatography and Mass Spectrometry, in order to determine the composition of a mixture and its individual pure compounds. This is done by analyzing the mixture of compounds using Gas Chromatography and the individual pure compounds by using Mass Spectrometry. Experimental Procedure: Obtain a screw cap and vial which will be ran in the Gas Chromatograph. Fill vial no more than half full of pre prepared unknown compound which is dissolved in either

The purpose of the gas chromatography experiment was to identify a compound by using its retention time on the chromatography column, then calculate the mole percentage of the compounds using the information from the relative peak area. During the gas chromatography experiment the retention time was calculated after the initial injection into apparatus allowed the compounds to retain in the columns. Throughout the experiment a polar column was used; therefore, the retention times increase as boiling

The extracted organic compounds were analyzed by use of gas chromatography coupled to a mass spectrometer. The sample extracts were injected into a Gas Chromatograph (Agilent 6890) fixed with a capillary column (DB-35MS). The Mass spectrometer used was an Agilent 5973 detector. The temperature of the column was first set at 70 degrees centigrade for 1 minute, then increased at a rate of 10 degrees per minute up to 300 degrees then held at that temperature for 10 minutes. The ion source & the transfer

report is to discuss about gas chromatography to determine chlorophenols using five differences extraction method that is ; solid phase microextraction, solid phase extraction, liquid phase microextraction, liquid-liquid microextraction, and dispersive liquid-liquid microextraction and choose the best extraction method that are suitable, easy to use, low cost and have more advantages than disadvantages. The separation techniques which collectively are called chromatography are widely been used for

limit of detection and detection threshold through instrumental analysis and human psychophysics, respectively. These values were then statistically compared to determine the superior method and inherent advantages/disadvantages were reviewed.1 Gas chromatography (GC) is a critical tool in a wide array of applications relating to food analysis, for the separation of various compounds (especially volatiles) within a mixture is typically essential in the characterization of inherent aroma compounds and

For this experiment, Gas Chromatography (GC) is utilized to observe the streo- and regiochemistry among four elimination products under either basic or acidic conditions. The reagents used in this experiment include primary and secondary alcohols which are in acidic conditions and dehydrobromintion of primary and secondary alkyl halides in basic conditions. When reacted the gas products are collected and undergo further analysis in the GC which will separate the product into its components and will

Part A 2. Gas Chromatography and High performance Liquid Chromatography (HPLC). Chromatography Chromatography is a collective term, essentially a process of separation of molecular mixtures. The analytical technique is widely used in forensic chemistry, in which a sample to be separated is dissolved in a solvent and the subsequent solution, the mobile phase, is passed through another material, the stationary phase. The technique is based on different rates of movement of constituents of a mixture

CHEM 4230, Tuesday Section Lab Memo Date: September 12, 2015 To: Dr. Stefanie Whitson From: Kerri Keller Lab Partners: Siri Alay, Landon Carver Subject: GC-MS of a gas, gas simulant, and octane booster Summary: Gas Chromatography- Mass spectrometry (GC/MS) was used to identify the major components of gasoline, a gasoline simulant, and an octane booster. GC-MS is an analytical method that separates mixtures into individual components and uses mass spectrometry to identify each component by

Fractional Distillation and Gas Chromatography Lab Report Introduction: Distillation is a method of separating two volatile chemicals on the basis of their differing boiling points. During this lab, students were given 30 mL of an unknown solution containing two colorless chemicals. Because the chemicals may have had a relatively close boiling point, we had to employ a fractional distillation over a simple distillation. By adding a fractionating column between the boiling flask and the condenser

Alkenes from Alcohols: Analysis of a Mixture by Gas Chromatography \ Cassie Coakley Lab partner: Julie Singh TA: Nicole Giddings Introduction/Background An elimination reaction involves getting rid of a proton from the Beta carbon to form a different bond, known as a Pi bond.2 In this specific reaction, the water molecules react with the unidentical beta carbons to initiate a leaving group and form the desired products: 2-methyl-1-butene and 2-methyl-2-butene. In both an E1 and E2 mechanism

Simple Distillation and Gas Chromatography The objective of the lab was to purify compounds that can be turned into gas at a reasonable temperature without using decomposition. Gas chromatography was also used to identify the proportions of the volatile compound in the different samples. The mixture of hexane and octane was assigned to me and my partner. The apparatus for simple distillation was assemble using a thermocouple, air condenser, distillation head, metal keck clamps, and a conical reaction

additionally concentrated with a nitrogen stream to 500 µl just before injection into the gas chromatograph and the components were analyzed by gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS) and GC-Olfactometry. Gas Chromatography (GC) A Hewlett-Packard (HP) 5890 Series II gas chromatograph equipped with a flame-ionization detector (FID) was employed. Helium was used as the carrier gas at a flow rate of 1 ml/min with a split ratio of 30:1. The column was 60 m x 0.25 mm i.d