Fruit Fly Lab Report

A) Their F1 offspring were 97 wild type quahaug flies. What is the genotype of these F1 flies??

This may have been the cause of the low numbers of white lozenge in the F2 generation of flies. However, the cause of white eyes is a defective red pigment gene and should not affect the vision of the flies, whereas the lozenge gene should have a greater affect due to it causing the malformation of the fly's eyes. Therefore the lozenge flies should have also been in lower than expected numbers, but it was found that they were actually in higher than expected numbers making the validity of this argument questionable.

The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.

It was decided that there would be 80 vestigial flies and 20 wild type flies to total to an initial population of 100 drosophila. Next, the flies were anesthetized flies using Fly Nap. The flies were counted out to reach desired ratio, sexing the flies making sure there are equal amounts of males and females to be sure there is ample individuals to allow successful mating. The fly’s food was prepared by taking a frozen rotten banana, cutting it in half, mashing up the banana meat, and mixing yeast into it. The

Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.

We started out with three populations; B, D, and G. In order for us to properly create controlled genetic crosses, we had to ensure that all the female flies were “virgins”.

Maintaining healthy cultures is essential in achieving the proper outcome expected for this lab. Before preparation of vials, or observation of flies, the workbenches and equipment, such as brushes, pipets, and measuring tools were wiped down with 70% ethanol ,and fly pads, as well as Co2 guns, were thoroughly disinfected with kim wipes. Hands were required to be thoroughly washed with anti-bacterial soap and completely dry, especially before preparation of food. Distilled water and other required sources were regularly changed in order to maintain

The expected number of wild type flies in the F2 generation is 734.25 and the expected number of shaven bristle flies in the F2 generation is 244.75. This, again, exhibits a 3:1 ratio of normal phenotype to affected phenotype.

Now you have determined some facts about the grounded allele and the trait that it causes. Given what you know, do you expect the mutant F1 flies to be homozygous or heterozygous for the allele that causes the grounded trait? According to your reasoning, if you mated two mutant F1 flies, what percentage of flies would you expect to be wild type versus mutant in the F2 progeny? Draw a Punnett square of this cross to justify your answer.

The gender in this experiment does not matter and so while testing the ratios predicted it seems to be correct because since I predicted a 2:2 ratio the numbers seemed to be really close to each other. When I crossed the flies the vestigial and purple mutations were dominant over the wild type because in the end of the crossing the filial products were 620 for the vestigial and purple mutation, while for the wild type it appeared to be 618. My null hypothesis is that if the genetic characteristic of the fruit flies tested are not linked, then the vestigial wings and the purple eye mutations in the alleles of the offspring flies will be recessive to the wild type flies. My alternate hypothesis is that if the fruit flies tested are linked, then the vestigial wings and purple eyes mutations in the alleles of the offspring flies will be dominant to the wild type flies. The observations that led me to this conclusion is that when following the procedure of the experiment, I observed that my hypothesis is correct because when I finished crossing the fruit flies.

We will use Punnett Squares to find out the genotype and phenotype in the offspring of the fruit flies. Our experimental hypothesis of this experiment is that the genes of the fruit flies are linked, where the body color of the fruit fly is dark (b) recessive or light (B) dominant and type of wing, normal wings (W) dominant and abnormal wings (w) recessive is linked. The null hypothesis is that there will be an unlinked ratio for the phenotypes if the loci was

As stated by the World Health Organization, “all fifteen HA subtypes and nine DNA subtypes have been detected in free flying birds”. (WHO, 2005, 12) They, in turn, provide a huge and highly mobile pool of genetic diversity.

METHODS: In this experiment, the instructor provided us with 30 ebony individuals and 20 wild type individuals. In order to get an exact amount of each type, we anesthetized the flies and counted them off by gently using a fine point paint brush. Then all 50 Drosophila were put into a population cage which had a lid that had six holes for the centrifuge tubes. Two food tubes and four clean, empty tubes were added on the first day. Each food tube consisted of half a cup full of food mixed with 6-7 milliliters of water. This was the fly medium. The food should turn blue once the water is added. Each tube was labeled with a number and with the date. Every two to three days we added one more food tube until all 6 tubes contained the fly medium. After all 6 tubes were filled, the following days after we exchanged the first food tube with a new food tube. At the end of the experiment, we fed the flies with a total of 8 food tubes. Then the flies were anesthetized, again. At the end of this four week lab, the number of living ebony and wild

When scoring the flies, five male and five females of the ebony and wildtype were counted out for a total of twenty flies. They were identified by looking at the hooks of the male flies under the microscope. After counting them, they were put into the culture vial and left in an area with temperature between 20 to 25 degrees Celsius for one

Moreover, the migration of individuals from one genetically distinct population to another is also an important way for alleles to be added to or subtracted from a local population. Whenever an organism leaves one population and enters another, it subtracts its genetic information from the population it left and adds it to the population it joins. If it contains rare alleles, it may significantly affect the allele frequency of both populations. The extent of migration need not be great. However, as long as alleles are entering