RIG-I recognizes bacterial ligands in glial cells. RIG-I is traditionally viewed as a viral pattern recognition receptor, however, more recently the pathogen ligands for RIG-I have been expanded to include bacteria nucleic acids. In non-CNS cell types RIG-I has been documented to identify bacteria RNA directly or DNA indirectly via an RNA polymerase III dependent mechanisms. RIG-I dependent recognition of bacterial nucleic acids was demonstrated to be pathogen and cell type specific. In glial cells the role and mechanism of RIG-I in pathogen detection has not fully been explored. We have previously demonstrated expression of RIG-I and the contribution of RIG-I to recognition of RNA and DNA viruses. However, the requirement of RIG-I for …show more content…
Confocal imaging of RIG-I recognition of bacterial ligands and nano-TNAs. In this proposal we will investigate the subcellular localization of bacteria and bacterial nucleic acids in relation to the cytosolic innate immune receptor, RIG-I. In order to accomplish this goal we will combine traditional immunofluorescence for RIG-I and cellular compartment protein markers with the use of commercially available products EU and EdU to label bacteria RNA and DNA, respectively. The Thermo Fisher Scientific products EU and EdU were originally developed to detect global RNA transcription and measure de novo DNA systhesis or S-phase synthesis, respectively. By growing bacteria in the presence of these products prior to infection of glial cells we can track not only bacteria but nucleic acid subcellular localization. This will provide insight into the accessibility of nucleic acids to host innate immune receptors. By innovatively combining immunofluorescence and nucleic acid labeling we will characterizing the localization of bacteria, bacterial nucleic acids, and RIG-I over a given time course providing valuable insight into the mechanisms of host cell recognition of bacteria in glial cells.
Nano-TNAs present significant advantages over traditional counterparts for therapeutic and adjuvant applications.
A. Compare and contrast afferent and efferent lymphatic vessels. Describe in one way in which they are similar and one way they are different.
Give the title of the presentation, name of the presenter, and when and where you attended the presentation (2 points).
This is proven by the survival of the bacteria in the LB/amp +pGLO dish, as the same bacteria that lacked the modification were killed by the ampicillin. However, we were unsuccessful in genetically transforming bacteria to be fluorescent. This is because in the LB/amp/arbo +pGLO dish, we were unable to locate any bacteria.
Bacteria belongs to a group of organism that lacks cell nucleus and other membrane bound organells. This group of organisms are called "Prokaryotes" and they follow the central dogma of molecular biology first proposed by Francis Crick in 1958 for protein synthesize. Protein synthesize is how instruction written by the bacteria DNA are being copied into a temporary form called mRNA and these mRNA are sent to
It's been said that Connor McDavid was the second coming of Sidney Crosby - and then it was said that Auston Matthews was the second coming of McDavid. So it has been every year with the first overall pick, and so it goes.
Who’s Boo? Is He A Monster? In the killing a mocking bird they talked about a man name Boo Radley. Now you’re probably thinking who the heack is Boo Radley.
In order to identify possible diagnostic targets, I will take advantage of the in vivo Microbial Antigen Discovery (InMAD) approach23. This methodology, especially useful vis-à-vis of pathogens that are present in very small quantities in the blood, is based on the immunization of mice using filtered serum from infected animals, in order to promote an immune response against circulating microbial antigens, but not against cell-related antigens. Then, the immunized sera are tested against bacterial lysates in 2D-western blots and positive spots are identified by mass spectrometry as potential diagnostic candidates.
Once the initial proteins are made, then eight complementary positive sense RNA strands are made from the eight negative sense RNA segments (at least in influenza A and B. . . influenza C has seven segments). These lack the 5' capped primer, as well as the 3' poly (A) tail found in the mRNA. From this cRNA, a negative sense RNA is produced. Various proteins then help this negative sense RNA exit the nucleus and into the cytoplasm of the host.
The ancient civilisation of Egypt has always been an admirable one, and until this day it is still full of mysteries. Despite all the discoveries that archeologists have made, there are many unexplained matters that they are still trying figure out. People considered pharaohs as they were descended from gods; however pharaohs knew that they are normal beings. Of Course they couldn’t show that to the people, and in order to keep their majestic image they turned to making such spectacular breathtaking status, tombs, and paintings.
Similarly, the fact that the DNA sensing pathway is quick to become anomalous is likely due to the initial detection of viral ssRNA by RIG-I (Pichlmair, et al., 2006).
Addiction is a chronic brain disorder conceptualized as a progression of spiraling dysregulation of brain reward systems that yield adverse physiological, behavioral, and sociocultural consequences. Addiction involves components of both impulsivity and compulsivity that produce characteristic, eccentric behaviors of drug addicts. Currently there are no efficacious treatments for substance use disorders, which behoove the development of novel pharmacotherapies. Recent studies have proposed Sigma Receptors to play a role in the mechanisms behind the addictive and neurotoxic properties of drugs of abuse, such as cocaine, methamphetamine, and alcohol. This chapter examines the mechanisms of the Sigma Receptor system in the effects of drugs and
The body responds differently to an infection, depending on whether it is a virus or a bacterial infection. Generally, viruses are considered to be more dangerous, but aside from generalities, there are also pathophysiological differences in the ways in which both viruses and bacteria (invasive organisms) infect human beings (hosts). When a virus is the agent that is infecting the host, the goal of the virus is to invade on a cellular level and replicate itself. “Once inside, the cells of the immune system cannot ‘see’ the virus and therefore do not know that the host cell is infected. To overcome this, cells employ a system that allows them to show other cells what is inside them” (Immune, 2015). The virus then uses the cell in order to make proteins and replicate itself, further compromising the immune system. The immune
Cellular Basis Of Disease: Why has the discovery of Toll-like receptors revolutionised our understanding of how the innate immune system works, and what is the therapeutic potential?
The bacterium evolved by loosing its genes converting it from a free-living microbe into a pathogen. It needs the host’s nutrients in order to survive. The bacterium latches itself onto the host epithelial cells by a 160 kDa type 1 pilli. The pilli, located on a specific organelle on the polar region of the
Previous experiments have characterised the role of SpnA as a cell wall anchored DNAse important for immune evasion through the destruction of neutrophil extracellular traps (NETs) (Buchanan et al. 2006). While small scale in vivo experiments in mice have been conducted (Hasegawa et al. 2010), a Galleria mellonella infection model allows for a larger scale screening of mutants to further refine the role of SpnA in GAS virulence. This is due to the lower cost, easy maintenance, small size and suitability of the wax worm (Ramarao et al. 2012).