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Elisa: Immune System and Serum D= Donor Essay

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Lab Report: 10 ELISA Purpose: This test is often used to see if you have been exposed to viruses or other infectious substances. It is frequently used to screen for present or past infections. Introduction: ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An ELISA or enzyme-linked immunosorbent assay is a method used in the laboratory to aid in the diagnosis of a wide range of diseases. This test is performed on blood or urine and is used for measuring the amount of a particular protein or substance in these bodily fluids, such as infectious agents, allergens, hormones or drugs. This test relies on the interaction …show more content…

Then you add 100ul of PBS to each well, and then remove it. After that, to each well using different tip each time add 100ul PBS to number 1, add 100ul B to number 2, add 100ul C to number 3 and add 100ml D to number 4. Incubate at 37C (Body temp) for 15 mins. After that, remove liquid using different tips. Next add 100ul PBS, and then remove using different tips. Add 100ul 2C AB to each well and incubate at 37C for 15 min. remove the liquid using different tips, add PBS then remove the PBS. Add 100ul of substrate to each well and incubate at 37C for 5 mins. A= HIV Antigens B= Positive control C= Donor 1 serum D= Donor 2 serum PBS= Phosphate Buffered Saline Results: A | B | C | D | 1 | 2 | 3 | 4 | - | + | - | + | Conclusion: For the first row, the results can back negative because that was the control. The second column was positive meaning for this control this is what a positive would look like. Now from the third column this donor serum is just negative for that antibody and the fourth row would be a positive for HIV antibodies. This test is medically important because it’s used for measuring certain hormone levels such as HCG in the pregnancy test, thyroid hormones, detecting dust and food allergies, detection of illicit drugs such as cocaine and methamphetamines. Also to measuring antibodies which are produced in auto-immune conditions such as Lupus and rheumatoid arthritis.

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