Determination of Sugars in Sports Drinks: A Spectrophotometric Analysis
By: Crystle Culling Student Number: 2686923 Class: Tuesday 12pm (odd weeks)
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1015MSC Lab Report
Crystle Culling 2686923
Abstract
The concentration of sugars in two well-known sports drinks, Powerade and Gatorade, were determined by monitoring an enzyme-catalysed reaction sequence involving the appearance of NADPH. Sucrose and glucose concentrations were calculated from the concentration of NADPH formed by the reaction of glucose-6-phosphate and NADP+. Spectrophotometric absorbance readings were taken at 340nm, this is because NADPH absorbs strongly at this wavelength, whilst NADP+ does not (1015MSC, 2010). The concentration of glucose and sucrose in
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Absorbance of NADPH at 340nm
1.2 1.0 0.8 0.6 0.4 0.2 0.0 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 Concentration (mM) y = 0.1897x R2 = 0.99
Calculations
To calculate the concentration of the sucrose and glucose after the first reaction, and the final concentration of glucose of the samples of Powerade and Gatorade, the Beer-Lambert equation was used.
Absorbance Difference
Beer – Lambert Equation: A = εcl
A = Absorbance difference = Molar extinction coefficient C = Concentration L = Path length
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1015MSC Lab Report
Crystle Culling 2686923
The molar extinction coefficient () was determined from the gradient of the line of best fit. Path length was neglected from the calculations as it was equal to 1cm. As the samples were prepared with a 1:100 dilution factor, this was included in the calculations (DF=100).
Sucrose plus Glucose Calculations (from Table 1 and Figure 1) Powerade Dilute = 0.36 / 0.1505 =2.39 mM/L C = Dilute x DF = 2.39 x 100 = 239 mM/L = 0.239 M/L Gatorade Dilute = 0.39 /
My hypothesis is that powerade would give that boost to make runners run fast. 10 runners are placed in a track to do a 2 mile run. 5 of them would be given powerade to drink in the mile mark while the other 5 are not given anything. The 5 runners that drank the powerade ran 2 miles in 10 minutes while the other 5 runners that didn’t drink anything ran the 2 miles in 12 minutes. As a result powerade helped the runners run faster for their times were faster than the ones that didn’t drink it.
Abstract: The concentration of Allura Red in red Gatorade was determined. It was done by using the equation M1V1 = M2V2 and comparing the concentration of the diluted solution to find concentration of the undiluted concentration. The concentration of the Allura Red in Gatorade was 1.238*10^-4 M.
With these absorbance numbers a concentration curve was constructed and the unknown solution was determined by finding the point of absorbance on the curve.
of sugar to 25ml of water and dissolve it. When we have the water at
Have you ever gotten a sports drink instead of a soda thinking you were choosing a healthier option? Well this might change your perspective. Powerade and Gatorade are just watered down soda; yes, it gives you electrolytes which is what you lose when you sweat, but that does not mean it is good for you. There are many other healthier ways of getting electrolytes than by just drinking a sugar drink. In the Powerade article written by the company does not argue about how water is not helpful to athletes and does not have the opposing argument. Although the gatorade article written by David R. Lamb they make it more persuasive than informational.
The materials used for this experiment were a glass beaker, a scale, 3 cups, three 3.5’’ bones extracted from a chicken wing, 100 mL of water, 100 mL of water mixed with 5.95 grams of sugar, and 100ml of red Gatorade, which contains 5.95 grams of sugar. The experiment was started by measuring out 100 mL water and placing it in a cup labeled “A”. The second step was to mix 100 mL of
Each sports drink contains 21 grams of sugar are contained in a 12 ounce bottle of Gatorade. With most bottles being 32 ounces, the consumer is actually ingesting 56 grams of sugar. Although this is not healthy, it is less grams of sugar than an average soda per ounce. “In fact, Berkeley researchers say the sugar in sports drinks may be contributing to the child obesity epidemic by increasing their caloric intake”. For consumers that are less active, the added sugar in their diet is not recommended. Weight gain from extra calories has also been found to be a problem for less active consumers. “For people who are not exercising for at least one hour, 5 days per week, water is the best bet for staying hydrated.” Experts suggest sports drink consumption should be monitored by children’s parents. The best source of hydration for children continues to be water (Schaefer). Unless a person is participating in vigorous exercise for a long period of time, water is the best option for
Sports drinks contain ingredients which have raised health concerns. The main ingredients in sports drinks are glucose, sucrose, and fructose or high-fructose corn syrup. (Insidetracker.com, 2012) Gatorade’s Glacier Cherry Perform drink, contain 41g of sugar per serving, which is equivalent to 10 teaspoons of sugar. (Griffith-Greene, 2014) Although sugar is necessary for the drink to taste good, sugar can have long term effects on your health. Too much sugar can over time lead to diabetes, high blood pressure and weight gain. (Hagy, 2007) As well as sugar, the acidity in sports drinks can dissolve teeth. These drinks are often full of sugar and sodium, which cling to the teeth, and eat away the protective enamel. People, who is drinking sports drinks, are often dehydrated, meaning they are low on fluids. Being low on fluids, affects the saliva production in the mouth, which protects the teeth by washing away products which cling to them. Being low on fluids means that there is less saliva to wash away
Time) because it had a correlation closest to 1. All three orders were graphed and a linear regression was used to see which graphed order was closest to 1. The order was determined by comparing the concentration and time to the mathematical predictions made using the integrated rate laws. Analyzing each graph and finding each correlation helped determine which graph was closest to 1. The more concentrated a solution is, the higher the absorbance of that solution. This is due to Beer’s Law. The law measures the absorbance of a solution by determining how much light passes through a solution. As the concentration of a solution increases, fewer wavelengths of light are able to pass through the concentrated solution. The absorbance at 60 seconds was 0.573 (Figure 1: Table1). To calculate the concentration (molarity), the Beer’s Law equation was used, Abs = slope(m)+b. Plugging in what is known into the Beer’s Law equation resulted in 0.573 = 3.172e+004 + 0, where the concentration is determined by M = 0.573-0/ 3.172e+004. So, the concentration at 60 seconds using the equation (M = 0.573-0 / 3.172e+004) was 1.824e-5 M. The 1st order graph resulted in k=0.006152 (Figure 1: Graph 1). Other groups also resulted in their decolorization of CV to be the 1st rate
Gatorade is a flagship brand of PepsiCo and has a commanding 75% market share of the sports nutrition beverage marketplace globally, being sold into 80 different countries according to the latest PepsiCo annual report published in late 2011. Gatorade's success in branding and product marketing has actually expanded the global market for sports nutrition beverages during the late 1990s and into the 21rst century. Recently however the company has faced many channels including product line extensions of the last decade which failed to deliver strong results (Pollack, 1997) and a more critical analysis of their ingredients as many of their beverages are sold in public schools (Tallon, 2009). Despite these challenges however, Gatorade continues to experience strong market share and growth. The intent of this analysis is to evaluate and provide recommendations for each of the four areas of the marketing mix including product, price, promotion and place or distribution.
The aim of the study is two-fold: to study the rate of absorbance with increasing concentration of glucose, and to measure the activity of enzyme yeast invertase on sucrose. In task 1, the product formation was measured using 3, 5-dinitrosalicyclic acid that reacts with glucose leading to a change in colour from yellow to reddish brown. In task 2, the enzyme kinetics of yeast invertase on sucrose was studied. The absorbance values of the corresponding volumes of the solutions were measured using a spectrophotometer. Michaelis-Menten curve and Lineweaver-Burk Plot were made in order to estimate the values of Vmax and Km
Powerade has become the official sports drink of the Australian, New Zealand, and Ireland rugby teams, the AFL, PGA Tour, NASCAR, NHRA, NCAA, the U.S. Olympic Team (excluding basketball and soccer, which have deals with Gatorade) and several other leagues and organizations around the world.”
In order to test the predictions of the hypotonic, hypertonic, and isotonic hypothesis for the solution made during the study, four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Beaker 1 is 250- mL and contained 150-mL of 10% sucrose with dialysis tubing A, while beaker 2 (a large bowl) contained 1% sucrose, with dialysis tubing B, C, and D. Tubing A contained 10-mL with 1% sucrose. Tubing B
As well, sport drinks have the ability to provide a subject with an increased amount of endurance for their exercise. One reason why sport drinks have this capability is due to the carbohydrates found in the beverages. These carbohydrates exist as either of two sugars, sucrose and glucose-fructose, which provide the energy for the body that makes sport drinks better to use during exercise than water (Tsintzas 155). Carbohydrate consumption allows athletes to work harder for longer periods of time and to feel better while doing the exercises. A recent study determined that runners who had only water to drink lost speed during the race compared to those who had sport drinks. Also, performance time was faster in runners who consumed the sport drinks as opposed to water. It was only during the final 17.2 kilometres of the race that the performance times began to differ and the runners who drank the sport drinks ran faster (Tsintzas 156). Furthermore, the exercise intensity was higher towards the end of the race from those runners who had the sport drinks. As with most performance enhancing implements, sport drinks have potential drawbacks.
Gatorade has emerged as the global leader in sports nutrition beverages by continually managing their brand to signify high energy, athletic excellence combine with one of the most efficient new product development and introduced processes in the beverage industry. As a result of being able to consistently synchronize these many components of their business so well, Gatorade today holds a 75% market share in the sports nutrition market globally today. Gatorade is owned by PepsiCo, which has made it possible for the company to sell in 80 countries today. Gatorade relies heavily on the PepsiCo distribution and retail network globally. Gatorades' revolutionary approach to managing branding for beverages has served to increase the total market size for this product category globally (Huang, Sarigöllü, 2012). Despite the continued widespread adoption of Gatorade as a healthy energy drink, the company has encountered resistance to its brand and the ingredients used for creating the many variations of Gatorade energy drinks (Tallon, 2009). Despite these setbacks the Gatorade brand continues to experience exceptional growth and stability over time.