Cell Growth Lab Report

Use a test tube holder to put the test tube into a container of boiling water for 5 minutes, or until the solution changes color.

I learned that anaerobic is an organism or tissue that is living in the absence of air or oxygen while aerobic is involves the organism or tissue receiving and requiring air. Furthermore I learned about the anaerobic cellular respiration that uses an electron acceptor rather than oxygen to complete metabolism using electron transport-based chemiosmosis. Also in this reading I learned about fermentation which is an anaerobic process in which energy can be released from glucose even though oxygen is not available.

Observation: no bugs were found except small, black, gnats were all close to the ground.

The guiding question relates to our background info because in order to know the effect the different solution had on the cell size we had to know our terminology, which was included in the background information. Section2: For this lab we had to follow certain procedures.

Experiment 1 In our first experiment, our question was which cell fraction would have the greatest amount of chloroplast present at an absorbance reading of 600 nm? To answer this question, we used centrifugation to separate out Pellet 1, Pellet 2, and Supernatant 2, and then we tested each of them at 600 nm three times to determine which one contained the most chloroplast. Based on our results for wet lab 1 we hypothesized that Pellet 2 would contain the most chloroplast. We predicted this because in wet lab 1, we learned that a homogenized solution contains all organelles, Pellet 1 contains large amyloplasts, and Pellet 2 contains chloroplasts and small amyloplasts (McAllister 83).

A 24-well plate was used to perform this experiment. For the start of the experiment, the 24-well plate had 12 wells that contained our HepG2 cells. A cell suspension was created, that contained 0.5-1.0x10⁶ cells/ml containing 10% fetal bovine serum. 500µl of the cell suspension was then added to each well in use. The well plate contained both the HepG2 cells, and cell suspension was then incubated in a cell culture incubator for 24 hours; this allowed for a monolayer to be formed in the wells. After the incubation period elapsed, the well plate was removed and placed inside a tissue culture hood. We then carefully removed all the media from the wells with the use of a P1000 pipette; making sure not to touch the bottom of the well plate, and

The hypothesis for this situation is that the plant that is not doing very well is that it is not getting the same amount of sun as the plant that was doing really well. Another possibility is that it’s not getting enough water as the other plant so it could not be doing as well because of those two

If feeding efficiency and reproduction have a direct correlation, and a population started with equal proportions of individuals with each of three feeding types, metal spoon, metal knife, and plastic fork, the frequency of the population with metal spoons as their feeding structure will increase in the next generation. While the frequency of metal knifes and plastic forks will decrease. Furthermore, since the organisms with the metal spoon feeding structure have a higher fitness level, this population will evolve by natural selection to a point where the metal spoon phenotype will be in abundant. While the organisms with metal knifes and plastic forks phenotypes will decrease in frequency due to the lack of reproduction. Eventually, if this population persist overtime, most of the organisms, if not all, will have the metal spoon phenotype, while very few, if not any, will have the metal knife or the plastic fork phenotype.

The purpose of this experiment is to show how different concentrations of Ampicillin affect Escherichia Coli Growth and how the bacteria become resistant to the antibiotic drug. Through a series of steps, which involves streaking agar plates with E.coli sample and application of ampicillin to the E.coli sample on the agar plate, the experiment yields a result that supports the hypothesis. The hypothesis acclaims that ampicillin would affect the growth of E.coli; measuring the zone of inhibition approves the claim in the experiment. The measurements of the zone of inhibition indicate that the generation of E.coli expands as the radius reduces. The reduction of the radius shows the E.coli population is reducing and becoming resistant. In other

The cells were washed with PBS and then incubated in serum-free media and treated with 2.5 μM of free sorafenib or an equivalent sorafenib concentration of SMA-Sor, 3 μM of free nilotinib or an equivalent nilotinib concentration of SMA-Nilo, DMSO or SMA for 48  h. Following treatment, media was collected, centrifuged to remove cell debris, and freeze-dried for 12  h. Samples were rehydrated and mixed with loading buffer (0.4 M Tris, pH 6.8, 5% SDS, 20% glycerol, 0.03% bromophenol blue). For zymography, samples were loaded on a 10% SDS-polyacrylamide gel containing 1  mg/mL of gelatin. After electrophoresis, the gels were incubated in renaturing solution (2.5% Triton-X-100 (w/v)) for 30 min at room temperature and then for 24  h at 37°C in a developing buffer containing 50 mM Tris, pH 7.5, 200 mM NaCl, 4  mM CaCl2, and 0.02% NP40. The gels were then stained with Coomassie blue R250, and regions without staining were indicative of gelatin lysis. The gels were briefly rinsed and scanned. For MMP-9 and isthmin-1 secretion, samples were loaded on a 10% SDS-polyacrylamide gel, and the expression of MMP-9 and isthmin-1 assessed by western blotting using anti-MMP-9 (D6O3H, Cell Signaling) and anti-isminth-1 antibodies (Biorbyt, San Francisco, CA,

After gaining some knowledge about bacteria, we were giving an investigating bacteria growth lab to do. Our objective was to observe the conditions required for bacteria to grow and to test the effectiveness of substances that may be antibacterial, disinfecting, and or sanitizing. My group and I began our procedure by gathering all the bacteria by swabbing our necks and mouths. After this, we inoculated the culture by rubbing the bacteria on the agar, a nutrient rich gel made from sea kelp, on the bottom side of the container where we grow bacteria, the Petridish. We hoped for the results to come back with little or even no colonies and an immense zone of inhibition around the tiny circle cut out of filter paper covered in toothpaste, Neosporin, and Chlorhexidine Gluconate 4% Solution.

The sixth lab I completed in Biology 101 taught me how autotrophs (self-feeders) and heterotrophs (other-feeders) make organic food molecules by using photosynthesis. Photosynthesis uses the energy from the sun and it is captured and stored in the chemical bonds of organic molecules. The sunlight consists of different wavelengths of light. In plant chloroplasts, they have different pigments that capture different wavelengths of light. Light capturing pigments in green plants are called chlorophylls and these absorb all the colors of light except green, which is mostly reflected. To separate molecules from each other according to their solubility in a particular solvent is done by the process of chromatography. This basically means that polar

In this lab write up, the population growth of a variety of living organisms was analyzed and upon analysis of graphs and background information given within the laboratory course notebook, the conclusion was made that abiotic factors are related to biotic factors in an ecosystem, if one factor is varied it can affect the entirety of the ecosystem. Abiotic factors have importance because they are directly correlated to how biotic organisms survive and the growth or decline of their populations. Abiotic factors refer to non-living physical and chemical elements in the ecosystem and biotic factors are living or once-living organisms in the ecosystem with the ability to reproduce and affect other organisms in the ecosystem.

On 07/11/2015 at the Lower Buckeye Jail (located at the above listed address) I was assigned as the floor officer for Tower 12. At approximately 1555 hours, Inmate Asad, Sami T188658 (V1) came out to Level 1 core stating that he had been hit in the back of the head multiple times inside his cell in T12 B pod. Inmate Asad was placed into the level 1 holding tank awaiting medical.

This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density