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Catalase Converting H2o2

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Chemical reactions take place in all living organism. All Most all chemical reactions require an enzyme. These enzymes act as a catalyst to help regulate the rate of a chemical reaction. Three small lab experiments were performed to find the optimum pH, temperature, and concentration of the enzyme and substrate. The lab used the reaction of Catalase converting H2O2 into H2O and O2. The reaction was measured by the production of oxygen, using quaiacol that turns brown when oxidized. A spectrophotometer set to an absorbance of 500 nm was also used to measure the reaction rate. The procedure can be found in the biology 1 lab manual on pages, 243,244,245. After preparing the tubes following the instruction in the procedure section, two test

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