Boiled Peas Lab Report

One gram of liver was sliced into pieces, then 10ml of homogenization buffer was added to it. The mixture of the liver and homogenization buffer was then placed into a homogenizer to make the liquid slurry. Once the slurry was made it was placed into a 50ml falcon tube to then be placed in the centrifuge. The slurry was centrifuged for 2 minutes at 2000rpm. Once the spin in the centrifuge was complete, the slurry had separated, the most dense particles to the bottom of the tube forming sediment and the lighter (liver succinate dehydrogenase enzyme) also known as the supernatant. The supernatant was extracted from the falcon tube and placed into a test tube. The tube was then kept at a low temperature, (in an ice bath) until it was required for use.

1. Record your hypothesis about what will happen when Biuret solution is mixed with the solutions from test tubes 1, 2, 3, and 4 here. Be sure to use scientific reasoning to support your hypothesis.

Wisconsin Fast Plants are known to be great educational tools for classroom experiments. They germinate quickly and are easy to take care of. These plants germinate after 1 or two days and have a life span of about 30 to 40 days. (Marin and Terrana, 2004).

3. We poured tube 1 with the solution in tube 3 to combine them. We repeated this for all of the tubes. Each of the tubes in step 1 was mixed with a tube in step 3, making there be 6 total test tubes with a solution in it.

We first started this experiment by obtaining twelve 15ml test tubes, in which we placed in a rack and labeled each with what

The next step of the project included preparing a Gram stain to discover the cell shape, arrangement, and if the bacteria is gram positive or

According to research done at the University of Florida (http://hendry.ifas.ufl.edu/HCHortNews_pHProblems.htm), the PH levels of the soil can affect the growth of a plant. In this research, the University of Florida was able to confirm that plants react better to PH levels that range from 6 – 7.5. In my experiment I will be pushing the plant's boundaries by watering the plants with plain, black (Name of coffee using) caffeinated coffee that has a PH level of (PH level). The plant I have chosen are lima beans. I chose the lima beans because they are a hardy plant.

tube was placed inside, then another test tube with an equal amount of substance would be placed

Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen and nitrogen and are bound more tightly to the paper than the other pigments.

Figure 1: . In 2010 A cartoon by Piraro about how fast food effect pigeons which representing the people who addicted on fast food.

For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.

The first experiment begun by filling a 600-ml beaker, almost to the top, with water. Next, a 10-ml graduated cylinder was filled to the top with water. Once water was added to the beaker and graduated cylinder, a thumb was placed over the top of the graduated cylinder. This would ensure that no water was let out and no bubbles were let into the graduated cylinder. Next, it was turned upside down and fully submerged into the beaker. Then, a U-shaped glass tube was attained. The short end of the glass tube was placed into the beaker with the tip inside of the graduated cylinder. Next, a 50-ml Erlenmeyer flask was received. After, 10-ml of substrate concentration and 10-ml of catalase/buffer solution were placed into the flask. A rubber stopper was then placed on the opening of the flask. After adding these, the flask was held at the neck and spun softly

Half of each tube’s contents are poured into a new test tube each respectively after the tubes are incubated for 1 hour. One set of tubes is tested for:

18) Looking into your microscope, you spot an unusual cell. Instead of the typical rounded cell shape, the cell has a very narrow middle separating two bulging ends. It sort of looks like the number 8! Then you realize that this cell is

Step 1 and 2 was repeated by using distilled water by replacing the test solution.