Introduction:
In this lab, we studied biomolecules. Biomolecules are molecules that are existent in all living organisms. The four biomolecules, we are learning in class are proteins, carbohydrates, lipids, and nucleic acid. The three biomolecules being addressed in the lab are proteins, carbohydrates, and lipids. Biomolecules are organic molecules because they contain carbon and hydrogen, as organic molecules always contain carbon and hydrogen. Biomolecules are important because they make up our bodies entirely and are the reason our bodies continue to function. Proteins have many functions. They make up hair and nails, and collagen, which lend support to ligaments, tendons, and skin. Enzymes, another type of protein, speed chemical reactions
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For testing for complex sugars, take the well plate and put about 2-3 mL of the substance being tested for complex sugars in one of the holes using either a pipet or a small spoon (depending on the substance).
Add about 3-4 drops of the iodine solution in the well plate hole using a pipet.
Thoroughly stir the iodine in with the substance using a toothpick.
Continue these steps for testing the next substances for complex sugars.
For testing for proteins, take the well plate.
Put about 2-3 mL in of the substance being tested for proteins in one of the holes using either a pipet or a small spoon (depending on the substance).
Drop about 2 mL of Biuret reagent in the well plate hole using a pipet.
Thoroughly stir the Biuret reagent in with the substance using a toothpick.
Continue these steps for testing the next substances for containing proteins.
For testing for lipids, take a piece of brown paper and put the substance being tested for lipids on it.
Let the substance sit for a moment and then hold it up to a light source to see if it has left a translucent spot.
Continue these steps for testing the next substances for containing lipids.
Data
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An error that occurred that came up in the class’s results was that when egg whites were tested for lipid, it came up slightly positive. This is due to human error. When separating the egg whites from the egg yolk, the egg yolk was accidentally dropped into the egg whites. Due to the fact that an egg yolk is a lipid, it caused for cross contamination between the egg whites and the egg yolk. Therefore, the egg whites come up slightly positive when testing for lipids and was clearly a human error. A way to improve these results would have been to not let the potato water sit for as long as it did and to be more careful when separating the egg whites from the egg yolk. One could do so by gently cracking the egg into a bowl and use a pipet to suck up only the egg whites. One concept I learned was the difference between simple sugars and complex sugars. How simple sugars are unattached monosaccharides and polysaccharides are attached monosaccharides. I had known that carbohydrates were in many things, but I did not know the different types, why some tasted differently, and why some supplied me with energy for a longer period of time. For example, how monosaccharides taste sweet and polysaccharides do not. Now I also know what to eat if I am looking for a long lasting energy source. In
Introduction: The biological membranes are composed of phospholipid bilayers, each phospholipid with hydrophilic heads and hydrophobic tails, and proteins. This arrangement of the proteins and lipids produces a selectively permeable membrane. Many kinds of molecules surround or are contained within
7)a) You would do the Benedict’s reagent test for simple sugars and the Lugol’s solution or Iodine test for polysaccharides and starch. To test the lipids you would use the Sudan iV test and Biurets reagent test for protein.
3. State the name and structure of the functional group for each type of biologically
1. Record your hypothesis about what will happen when Biuret solution is mixed with the solutions from test tubes 1, 2, 3, and 4 here. Be sure to use scientific reasoning to support your hypothesis.
2. Measure out 25 to 45 grams of one of the four metals on the trough. This may require several "clicks."
1. State a commercial use for biochemical testing as performed in this online laboratory. (5 points) face wash! To be able to test what different ingredients within the facial cleanser and how they will effect the areas, and chemicals of the skin composition.
The purpose of this lab was to test different substances using various procedures to see what biomolecules were present and ultimately find out what restaurant Anna Lyza had eaten at before she died. For the first control test, we used vegetable oil to test for lipids. So, if the solution does not contain lipids, it does not become translucent when placed onto a paper bag square and held up to a light. So, it is a negative result. However, in the presence of lipids, the solution will become translucent when placed onto a paper bag square and held up to a light. Therefore in this case, the result is positive. On the other hand, we used albumin egg to test for proteins in another control test. If the solution does not contain proteins, it will not experience any color change and so it is a negative result. When there are proteins existing in the solution, it will turn bluish/purplish and for this reason it is a positive result. Furthermore in the third control test, we used dextrose to test for simple carbohydrates such as glucose. If the solution does not contain simple carbohydrates, it will not undergo any color change and will remain a blue color. So, it is a negative result in this circumstance. If there are simple carbohydrates present in the solution, the solution will turn reddish and so the result is positive. For the last control test, we used starch solution to test
Thereafter I used a pipette to meticulously drop in water up to the 250ml mark.
All living things contain some form of organic macromolecules including: Lipids, proteins, carbohydrates and nucleic acids. All of these organic molecules are alike in the sense that is they are made up of bonded elements such as carbon, hydrogen, oxygen, and to smaller quantities nitrogen, phosphorus and sulfur. The macromolecules each contain large long chains of carbon and hydrogen atoms and often consists of repeating smaller molecules bonded together in a repeating pattern (polymers). To test whether a specific solid white substance is a protein is simple due to the unique chains found in the respective type of macromolecule. To test if the substance is a carbohydrate, two tests could be performed on separate samples of the object. First,
Submerge the graduated cylinder in the plastic tub so that it is completely filled with water. Hold the open end of the graduated cylinder and move it vertically upside-down where the open end of the graduated cylinder is still submerged in the plastic tub. Clamp the graduated cylinder the ring stand of the lab table to keep it in place. perforate a hole in the top of the rubber cork for the solution container. Cut a straw the length of about four inches. place the straw inside of the rubber cork hole. Set up your timer for two minutes.
Analysis: In this experiment, every substance tested positive for at least 1 organic compound. Corn oil tested positive for lipids. Honey and oats tested positive for starch. Honey, apple juice, and unknown 1 tested positive for sugar. Egg white, gelatin, and unknown 2 all tested positive for proteins. So, not a single substance didn't test positive for at least 1 organic compound. All the indicators used in this experiment change color when in the presence of a compound; therefore, they chemically react with the compound. No errors were made during this experiment
Course description: The chemistry and biological properties of amino acids, proteins, nucleic acids, lipids, carbohydrates, and vitamins. The course includes laboratory and tutorial sessions.
4.Measure 35mL of warm water and add them into each of the 4 test tubes at about roughly the same time. It is essential that the water is warm. Do not seal the test tube.
Perform a series of accurate tests on biological molecules to detect the presence of carbohydrates and proteins, as well as the action of an enzyme on specific molecules.
The most common macromolecules found in living organisms are lipids, carbohydrates, proteins and nucleic acids. Briefly, the meaning of macromolecules is that they normally contain two or more molecules in them and their main functions are to store energy, information and much more. Most foods are known to be combinations of macromolecules. While some of these compounds can be detected by taste tests, many cannot. Scientists then use certain tests to determine the presence of macromolecules.