Aseptic Technique & Culturing Microbes
Purpose: To learn and employ aseptic technique and basic forms of culture media as well as become familiar with the basic requirements of microbial growth and the methods used to control microbial growth.
Procedure: Obtained a small Styrofoam cooler placed two small light bulbs in side and observed temperature over 24 hours to ensure temperature could be maintained between 98-100 degrees. Using a 10% bleach solution I then cleaned my work area. Transferring one capsule of L. acidopholis into a tube of MRS broth using the aseptic transfer technique then marked a line on test tube to record sediment. Labeled tube of nutrient broth S. epidermidis, then using a sterile swab obtained sample of
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Bacterial refers to killing bacteria and bacteriostatic refers to inhibiting the growth of bacterial cells. Sterilization is the complete destruction or elimination of all viable organisms. There is no degree of sterilization either it is or is not. Disinfecting is lowering the number to safe levels
B. List five sterilization methods, how they work, and what they are used for. 1. Filtration – 2. Boiling – 3. Autoclaving – 4. Dry Heat – 5. Incineration -
What is pure culture? Why is it important to work with a pure culture?
A pure culture is one in which all organisms are descendants of the same organism, it is important to work with a pure culture due to microorganisms usually exist as mixed populations so to be able study, characterize, and identify microorganisms they must be in the form of a pure culture.
What is aseptic technique? Why is it so critical?
Aseptic technique is the method of preventing unwanted microorganisms from gaining access, this is crucial for allowing the transfer of growing organisms to a sterile medium with out introducing any outside contaminants.
C. Describe three common forms of growth that you are likely to see in a broth culture?
Pellicle – mass of organisms floating in or on top if broth
Turbidity – organisms appear as a general cloudiness through the broth Sediment – mass of organisms appears as a deposit at the
After this was determined, the bacteria was transferred into a nitrate reduction broth tube. Nitrate broth contains a durham tube which is used to indicate fermentation, nutrients essential for
An unknown bacterium was handed out by the lab instructor. The methods that have been learned so far in identifying bacteria were applied to this unknown. Procedures were followed as stated in the lab manual and biochemical test handouts. The first procedure that was done was a gram stain followed by a streak of the unknown on a TSA plate in order to determine the gram reaction and observe the colony morphology. After that, specific biochemical tests were performed for gram positive, since unknown number five was determined to be gram positive rod. The other tests were performed in this order: Mannitol Salt (MSA) streak, Blood Agar streak, Catalase test, Nitrate Reduction test, and Phenyl
The sole purpose of this project was to identify an unknown bacteria sample #7. Many tests were carried out to determine what this unknown was. Aside from a microbiology lab, understanding and identifying various organisms are important in disease processes, pharmaceutical arenas, and even in the industrial field. Proper lab techniques, including aseptic technique were used throughout the process of identification.
Introduction The main purpose for completing the experiment is to understand bacterial growth. In order for bacteria to grow effectively, two important factors are required, physical and nutritional. Physical factors include temperature, pH, osmotic pressure and gaseous requirements1. Bacterial growth is temperature sensitive.
Background information: In this lab you will be looking at the growth of bacteria under different conditions to see the how populations of bacteria grow. Read about cells in the text or e-text. For everyone, in your e-text read chapter 13, sections 13.3 and 13.4 to learn more about bacteria. Then answer the questions below.
After gaining some knowledge about bacteria, we were giving an investigating bacteria growth lab to do. Our objective was to observe the conditions required for bacteria to grow and to test the effectiveness of substances that may be antibacterial, disinfecting, and or sanitizing. My group and I began our procedure by gathering all the bacteria by swabbing our necks and mouths. After this, we inoculated the culture by rubbing the bacteria on the agar, a nutrient rich gel made from sea kelp, on the bottom side of the container where we grow bacteria, the Petridish. We hoped for the results to come back with little or even no colonies and an immense zone of inhibition around the tiny circle cut out of filter paper covered in toothpaste, Neosporin, and Chlorhexidine Gluconate 4% Solution.
Epidemiology – the science that studies when and where diseases occur and how they are transmitted. CDC, Atlanta, tracks and traces diseases. ONE World (the idea that it’s all in one place, it could happen anywhere; locally, states, large counties track the incidences and occurrences of disease)
Sidney LeVine Ms. Kim Science 6 Jan. 14 2015 Reverse Osmosis filtration method Paragraph 1: Overview Reverse Osmosis filtration method, and explain what “reverse” and “osmosis” means, explain theseus, explain Paragraph 2: Explain why it is necessary for this filtration method to be used (disease/bacteria removed), and how if affects the people using it and the people applying it to their everyday lives. Paragraph 3: Pros and Cons overviewed with explanation Paragraph 4: Review Reverse Osmosis, briefly cover highlights from text, finished. Theseus statement: Reverse osmosis is an effective filtration method that positively affects the surrounding community by giving them guaranteed clean tap water. Paragraph 1: Reverse osmosis is an effective filtration method that positively affects the surrounding community by giving them guaranteed clean water.
These media can also help facilitate metabolism or inhibit. These options are in a form of selective media, meaning the goal is to isolate certain bacteria. While the other type of media, called differential media, helps as a general growth medium, but also helps show the differences in metabolic growth between chosen organisms. Peter, the microbiologist, is again tying the relationship between metabolism and microbiology media by saying, “These respective exergonic (energy-yielding) and endergonic (energy-requiring) reactions are catalyzed within the living bacterial cell by integrated enzyme systems, the result being self-replication of the cell. The capability of microbial cells to live, function, and replicate in an appropriate chemical milieu (such as a bacterial culture medium) and the chemical changes that result during this transformation constitute the scope of bacterial metabolism” (Jurtshu, 1996). That is the main functioning behind culture mediums in this specific lab and in general. Having microbiological mediums allows for bacterial outside a host and can help further study of the organism. Further studying leads to a better understanding on how to treat bacterial infections or how to prevent it. The history behind this lab and its contents are rich. The foundation of taxonomy with regards to bacterium can be gathered from this lab alone along with its findings. In 1966 Edwards and Ewing's
A mixed culture of two unknown bacteria was provided by the instructor. The methods used for
The other type of process for bacterial growth is anaerobic respiration, these bacteria grow only if there is oxygen present. The final requirements for growth are ph. level and time. Each type of bacteria flourish in certain levels of ph., for example acidophiles grow best with a ph. level under 5.5. Then if the requirements for the bacteria are met more efficiently then the bacteria grows at a more rapid pace.
THEORY: Nutrient agar media is used to facilitate the growth of the wide range of non-fastidious bacteria.
A number of factors must be considered to enable the use and control of the MICP process in field applications, including the concentrations of bacteria solution, the concentrations of the chemical solutions, in addition to methods to introduce the bacteria and these chemical solutions to the soil.
Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a
Microbial cultures are foundational and basic diagnostic methods used extensively as a in research tool in molecular It is important to have pure culture isolation. A pure culture is a population of cells as a multicellular organisms growing in the absence of other species or types.