Antibacterial Colonies Lab Report

Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).

The main idea of this experiment was to correctly identify the unknown bacteria, #3. Identification of unknown bacteria yields multiple benefits in many different areas in the research of microorganisms. In this experiment I performed many different test dealing with things such as the presence of enzymes, fermentation abilities and different chemical reactions. Observations made from the tests were then compared to a gram negative unknown chart in order to identify the bacteria. Based off of my results and the chart, I concluded the bacteria #3 was the bacteria Escherichia coli. E. coli is most commonly found in the intestines of warm blooded organisms. Most E. coli strands are non pathogenic however, there are strands

E. coli is a bacterium capable of being transmitted from one person to another. To ensure that the bacteria used in this experiment were not transmitted to other places, items, or organisms, several safety protocols were followed.

The purpose of this experiment is to investigate the effects of various household products may have on bacterial growth. The bacteria that was chosen as a test subject is Micrococcus luteus, which is generally considered a harmless bacterium unless the host has a weakened immune system. Among the household products that a set of groups will test are common cleaning and disinfecting products such as rubbing alcohol, Betadine, Germ-X, Neosporin, liquid hand soap, and Lysol cleaner. In the experiment, the bacterium, Micrococcus luteus, is to be swabbed and grown in a Petri dish divided into four sections with agar in each. After that, a small circle of thin paper is soaked in the four household items. Then, a piece of parafilm

When determining which bacteria I wanted to use for this experiment I had to decide on E.coli bacteria which is gram negative and staphylococcus a gram positive bacterium. These were chosen because they are safe enough to grow in a college laboratory and were supplied by the technician. Gram negative bacteria has an outer membrane making it more resistant to antiseptics and antibiotics, it also makes it more fatal to the human host it is inhabiting. Whereas gram

For the final lab activity, I randomly selected a test tube containing an unknown bacterial microorganism #8 which was in liquid broth form. The purpose of this lab is to utilize an assortment of lab techniques that we have learned throughout the semester and understand how the differences in results could help us in the identification process of an unknown bacteria. The case study corresponding with my unknown is an 86-year-old male nursing home patient who has been suffering from fever, chills, and bladder pain, associated with use of a catheter. To achieve accurate test results, proper techniques need to be done thoroughly and carefully. By forgetting to do something as simple as flaming a loop or needle before and after each use may lead to false results and conclusions.

The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment

In this lab experiment, students had to create a growth curve for E. coli. The E. coli growth curve would illustrate the progression of the population of E. coli a set time period. In this case, the growth curve depicted the population of E. coli over a 12-hour period. The growth curve for E. coli was created from the absorbance levels, the optical density(OD), recorded from the spectrophotometer.

Mouth Swab - looks like coccobacillus; plaque: looks like single baccilus; Thick multi-layered – positive gram stain; colorless moving particles that resemble droplets clustered together

This experiment illustrates the importance of handwashing and proves that hand washing is worth it. Since our hands are constantly coming into contact with ourselves and others, touching surfaces, grabbing objects, being sneezed into, etc., keeping our hands clean is one of the most effective, yet simple way we can take to avoid getting sick and spreading germs to others. Many diseases and conditions are spread by not washing hands with soap and clean, running warm water. “The human skin is a host to anywhere between 10,000-10,000,000 bacteria per square centimeter and since health care providers come into contact with pathogenic bacteria by being engaged in patient care, hand washing can reduce the risk of spreading diseases (page 3).” The objective of the experiment is to test the effectiveness of hand washing and demonstrate normal flora. This report presents the procedures and materials for the experiment, the experiment's results, and an analysis of those results.

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

Life on this planet began with microorganisms. Through millions of years microorganisms have found ways to successfully adapt and survive. These adaptations have created a wide biodiversity, allowing them to basically populate in all places. Why are these microbes so important? Because they shape the history of our world. Some microbes can be deathly to humans while some others are favorable, for example, bacteria that lives in the gut of both humans and animals and helps during the process of digestion (Alfred Brown & Heidi Smith, 2006). Understanding these interactions help scientists to find ways to protect humans from potential deathly pathogens. In order to observe microbes, microscope proficiency and microorganisms’ identification are crucial skills in a microbiology lab. During this laboratory session, samples of environmental and human organisms were inoculated into two different rich media and incubated to their according temperature. After this, appropriate use and calibration of the microscope was performed. Lastly, morphology and size of different species of bacteria, algae, fungi and protozoan were recorded.

This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a

This assignment will encompass how the results of the personal hygiene and susceptibility microbiology experiments provide a framework for the basis of the NICE Clinical Guidance (CG139) on Infection and how crucial the reasons for the hand wash protocol and hygiene is in all healthcare settings.