JEOL 1200EX II

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School

Rumson Fair Haven Reg H *

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Course

101

Subject

Mechanical Engineering

Date

Nov 24, 2024

Type

pdf

Pages

3

Uploaded by CoachRiverTiger30

Report
P RACTICAL E XAM ON JEOL 1200EX II F OR TEM L ABORATORY C OURSE & C HECKLIST OF R EQUIREMENTS FOR U SING THE TEM U NSUPERVISED User: Date: Examiner: Users must score 100% to use the JEOL 1200EX II Unsupervised. Users can be checked out any number of times until her/his score is 100%. Scope must be used at least once a semester to maintain unsupervised status. YES NO 1. Does user know all steps, in order for starting up microscope? Pre-start Checklist : Power On, Vacuum operating at High Vacuum/Green LED Log Book Signed in LN 2 trap filled - focusing scope & viewing window covered Spot Size on 4 Bright-LED On All deflectors off (Obj-Stig, Cond-Stig Dark Tilt, Bright Tilt and Image-Shift) Condenser aperture selected Objective aperture out Intermediate aperture out Function to Mag-1 Magnification at 10,000x Room lights G G 2. Can user locate, center and saturate filament correctly? Beam Generation : Filament fully CCW Step up High Voltage Bias Set Brightness (CL#3) Set Wehnelt (Filament) Image Beam Alignment : Beam Centered, Spot 1 vs Spot 4 Lenses Cleared Gun Tilts Set Condenser Lens Stigmated Condenser Aperture Centered Filament Saturated Beam Spread CW LN 2 trap topped off G G G G 3. Can user recognize objective aperture alignment, locate and center Aperture? Beam Centered Function Diff used to located aperture Aperture centered G G
4. Can user correctly insert and remove specimens? Magnification reduced [10K or lower] Beam Spread CW Specimen trans controls centered at X=0 and Y=0,and tilt=0 E Loading Specimen Holder : Specimen rod holder used Closed forceps used to open & close securing plate Grid loaded specimen-side-down Specimen rod not touched Lint on o-ring can be removed with naked finger Inserting Sample : Rod inserted with guide pin aligned Rod pushed and held while roughing pumps starts Air lock pumped out TWO times (for tungsten) Rod turned 90 E CW to stop and rod eased into column Removing sample : Specimen trans controls centered at X=0 and Y=0,and tilt=0 E Specimen properly retrieved from column Specimen plates closed Rod returned to air lock G G G G G G G G 5. Can user correctly change between grid position 1 & 2? Magnification reduced [10K or lower] Beam Spread CW Current grid to X=0, Y=0 [Page-2] Function to Low-Mag and Mag at 120X Swing Objective Aperture out. Move from one position to the other. Return: Objective Aperture in, Function to MAG-1, converge the beam. G G 6. Can user correct Objective Lens Astigmatism G G 7. Can the user correct use the digital camera system? Camera power ON Login to workstation and start MaxIM DL 5 software CCD Temperature to > -9 C Modify Annotations to show correct Accelerating Voltage Sample image focused & stigmated Adjust Brightness to fill the screen Lift Screen before capture image Set Magnification and mag bar on image Convert to Grey Scale: Save image - Check (Contrast /Brightness change) Screen down Can Use Video window G G
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