Column Chromatography Report (1) (1)

Q: Use the mass spectrometry data from Table 3.1 to determine the RMM for the protein. +14 +13 +12 Table 3.1 Data for Figure 3.5 +15 Measured +11 m/z 1193.417 108.381 1085.036 90.353 +16 994.683 76.437 +10 918.246 65.517 Figure 3.5 Example mass spectrometry data for an intact protein of theoretical RMM 11924.2 (solid bars) with calculated charge (z) indicated. The shaded peaks indicate possible impurities. 852.729 56.782 795.947 49.685 746.262 800 900 1000 1100 1200 m/z Relative abundance

PERFORM THE FOLLOWING EXERCISES DOING MOL - GRAMS STECHIMETRIC ANALYSIS

Column Chromatography Alumina Chromatography Mixture 9:1 Hexanes:Ether 8:2 Hexanes:Ether 1:1 Hexanes:Acetone Amount Used 3.962 g 0.143 g 9.50 mL 9.50 mL 11.00 mL Additional Observations (Color, etc.) BIU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BIU X₂ X² →

Qno3 solve please dont reject. Fill all and asignmnt list also fill dont reject okk

use the following chromatogram and table as your data.       What is the %composition of the Vitamin A peak in the sample?     78.43%        77.73%     7.867%       72.74%

analyte concentration(C)(mg/ml) injection volume (ul) elution time (time) peak DAD signal(mAU) caffeine 1 1 4.67 302.85 aspartame 5 1 7.53 15.83 benzoic acid 1 1 8.14 89.98 saccharin 1 1 1.91 84.86 mixture(add everything above with 1:1:1:1 ratio)   1 4.47 69.58 How to get the concentration of the mixture in this case?

The following data are from the HPLC assay of paracetamol 500 mg tablets using the calibration curve method: - Weight of active ingredient in powdered tablets taken for analysis: 180 mg • Initial extraction volume: 200 mL - Dilution steps: 10 mL into 100 mL, then 5 mL into 100 mL - Linear equation: y = 12000x - 50 %3! - Area of chromatographic peak for sample is 4750. What is the theoretical concentration in final solution in mg/100 mL? (2 decimal places). Please do not write the units, just numerical value. Answer:

Can you please fill the rest of tghe data based on the on inforamtion you have and  answer  Average concentratuon of NaOH  Standard deviation of concentration

anch of the following components cannot be detected by gas chromatography? 4 Essential oils Alcohols b) Volatile compounds d) Non-volatile compounds 13- is measured as time elapsed from the point of injection to the peak maximum? a)-Column efficiency d)- Delectability c)-Retention time 14- Which of the following can be used to detect the components in gas chromatography a) non-volatile compounds b) volatile compounds c) Salts d) b&c 15-Which of the following methods is often referred to as mechanical separation processes. a)-Gas Chromatography b)-column chromatography c)- Sedimentation 16- For obtaining better resolution in TLC. and then developed with a second solvent at right angles to the first a) Stepwise b) Descending e) Ascending 17- This is defined as transport of electrically charged particles in a direct-current electric field a) ion exchange chromatography b) Electrophoresis c) ultracentrifuge d) exclusion 18-This figure indicates to schematic Diagram of a) Gas chromatography…

please help me find average and standard deviation

two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used?

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1

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6-9 and 11-17

50 Percentage Red No. 40 Soln 100% 80% 60% 40% Table 4.1: Dilutions of Red No. 40 & Absorbance Volume of Red No. 40 Soln (mL) 10me 8mL опи 4mL Percentage Red No. 40 Soln 100% 80% 60% 40% 20% Volume of DI Water (mL) OML 2mL LIML M UML 8ML Table 4.2: Dilutions of Red No. 40 & Absorbance Concentration Red No. 40 2. Report the wavelength with the maximum absorbance of Red No. 40 dye. Absorbance 20% 2ML Part A: Making a Calibration Curve for Beer's Law 1. Calculate the concentrations of each of your diluted Red No. 40 solutions and add these to Table 4.2. Show at least one sample calculation in the space below. 0.251 0.192 Total Volume 0.181 0·119 0.060 (mL) 10 mL 10 mL 10 mL 10 mL 10 mL

1,3,5 what the correct answer?

A2

Only qno2 solve full accurate with diagrams follow all instructions of qno2. ok

2

Question 24 use the following chromatogram and table as your data. Relendon Time 10 15 20 25 35 Mntes VWD: Signal A, 284 nm Retention Area Identity Time (min) 7.867 3214697 Vitamin A 8.493 15687 19.410 140354 19.710 64346 20.803 884315 Vitamin E 21.640 36745 22.100 63054 What is the %composition of the Vitamin A peak in the sample? O 7.867% O 77.73% O 78.43% O 72.74% E8.403 is 20.803

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The uses of thin layer chromatography are: 1) To identify unknown compounds 2) Determine purity of products 3) Analyze reaction mixtures 4) To waste time in lab 5) Determine the melting point of the product U 6) Put an astronaut on the moon.

Table 1: Chromatography Results Mobile Phase: Distilled Water Solvent front distance (cm): 8 cm   M&M Candy Separated Spot Color Migration Distance (cm) (distance to center of spot from origin) Red M&M Red 7.5 cm             Green M&M Yellow 5.3 cm   Blue 7.7 cm       Blue M&M Blue 7.8 cm             -please help with this part Calculations/Results:             Table 1: Results with Distilled Water M&M Candy Separated Spot Color Rf Value   Red M&M                 Green M&M                 Blue M&M                             Rf = Distance traveled by component in a given time/                       Distance traveled by solvent in same time             Show work with units for one sample calculation.

Take into account the sample was DILUTED TWO FOLD.

Compare the quantities For a normal phase chromatographic technique: KA > KB Choose A if Polarity of A > Polarity of B B if Polarity of A< Polarity of B C if % both is equal D Cannot Be Determined

Why chromatography is an important tool for analysis of organic compounds?