Selective and Differential Media Questions

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University Of Arizona *

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181R

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Biology

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Apr 3, 2024

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docx

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Selective and Differential Media Questions Mannitol Salt Agar Escherichia coli Growth on Mannitol Salt Agar (yes or no) Mannitol Salt Agar Medium Color (red or yellow) 24-hour observation 48-hour observation 72-hour observation Staphylococcus epidermidis Growth on Mannitol Salt Agar (yes or no) Mannitol Salt Agar Medium Color (red or yellow) 24-hour observation 48-hour observation 72-hour observation Paste the Required Photos Below: 1. What chemical in MSA makes it selective media? How? 2. What chemical makes MSA a differential media? How? 3. Did you observe any growth on your MSA plate? Did you observe any color change on your MSA plate?
4. What color does MSA appear with the growth of Staphylococcus aureus ? What color does MSA appear with the growth of Staphylococcus epidermidis ? 5. Below is a photo of an inoculated, incubated mannitol salt agar plate. Is the organism growing on the right hand side of the plate Gram-positive or Gram-negative? Does the organism on the right-hand side of this plate ferment mannitol? 6. How do we identify hospital staff as carriers of Staphylococcus aureus ? 7. How is S. aureus transmitted? 8. How can MRSA infections be prevented? 9. The microbiology department is celebrating the end of the school year in May by holding its traditional picnic. The speeches drag on for a couple of hours, but finally all the faculty and students can dig into the food: chicken salad, tomatoes, onions, salad, and custard pie. By evening, the whole department, except for two vegetarian students who did not eat the chicken salad, is stricken with nausea, vomiting, retching, and abdominal cramping. Several individuals complain of diarrhea. One patient shows signs of shock (low blood pressure). Blood and stool samples are collected from patients, and an analysis of all foods served at the meal is conducted. Bacteria can cause gastroenteritis (inflammation of the stomach and intestinal tract) either by colonizing and replicating in
the host, which is considered an infection, or by secreting toxins, which is considered intoxication. Signs and symptoms of infections are typically delayed, whereas intoxication manifests within hours, as happened after the picnic. Blood samples from the patients showed no signs of bacterial infection, which further suggests that this was a case of intoxication. Since intoxication is due to secreted toxins, bacteria are not usually detected in blood or stool samples. MacConkey agar results ruled out E. coli , Salmonella , and Shigella . Analysis of the chicken salad revealed an abnormal number of gram- positive cocci arranged in clusters. A culture of the gram-positive cocci releases bubbles when mixed with hydrogen peroxide. The culture turned mannitol salt agar yellow after a 24-hour incubation. A. What Genus and species of bacteria gave the picnic goers food poisoning? B. Is the intoxication caused by an exotoxin or endotoxin? Please explain. MacConkey Agar Escherichia coli Growth on MacConkey Agar (yes or no) Colony Color (colorless or pink) 24-hour observation 48-hour observation 72-hour observation Staphylococcus epidermidis Growth on MacConkey Agar (yes or no) Colony Color (colorless or pink) 24-hour observation 48-hour observation 72-hour observation Paste the Required Photos Below: 1. What type of bacteria are inhibited from growing on MacConkey agar? What ingredients in MacConkey agar selects against those bacteria?
2. What ingredient makes MacConkey agar differential? 3. What color colonies are produced on MacConkey agar by organisms that ferment lactose? 4. Below is a photo of an inoculated, incubated MacConkey agar plate. Is the organism growing on this plate Gram-positive or Gram-negative? Does this organism ferment lactose? 5. Did you observe any growth on your MacConkey plate? Did you observe any color change on your MacConkey plate?
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