BME346H1S_2019_BIOMEDICALENGINEERING_OMICSTECHNOLOGIES_E

pdf

School

York University *

*We aren’t endorsed by this school

Course

1C

Subject

Biology

Date

Dec 6, 2023

Type

pdf

Pages

24

Uploaded by MinisterQuail2783

Report
UNIVERSITY OF TORONTO FACULTY OF APPLIED SCIENCE AND ENGINEERING FINAL EXAMINATION, April 26, 2019 DURATION: 2 and Y2 hrs Third Year - Biomedical Systems Engineering BME3461-11 S - Biomedical Engineering & Omics Technologies Calculator Type: 2 Exam Type: B Examiner — N. Matsuura Page 1 of 24
Instructions for this Exam: Wireless communication (i.e., cell phones, tablets, etc.) and other electronic devices are not permitted. These must be turned OFF and kept in your bag/backpack. You are NOT permitted to retain these devices in your pant or coat pocket, or on your desk. Textbook, lecture and lab materials are not permitted on your desk. Pencil (or other) cases are not permitted on your desk. Drinking and eating are prohibited. Place your Photo ID (Tcard) at the top right corner of your desk for the duration of the exam. The maximum amount of marks for this exam is 100. The number of marks available is indicated in parentheses ( ) at the end of each question. Please adhere to specified sentence limits. One mark will be deducted for each sentence over the specified limit. This exam is double-sided. Only answers written in space below questions will be considered. If necessary, use blank sheets provided for calculations or draft JiIWkJ1! The "time remaining" will be announced at 10 minutes and 5 minutes before the end of the exam. You may not leave the exam room during the first hour (2:00 to 3:00), or after the 5 minutes remaining announcement. You have 2.5 hours to complete this exam. Page 2 of 24
BME3461-11I EXAMINATION 2019 1. Fill in the table below. (6 marks) -Ome What is it made of? What does it show? Genome Transcriptome Proteome Metabolome Epigenome Phenome Page 3 of 24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
2. In one sentence, what is a "biomarker?" Give 6 examples of biomarker sources. (4 marks) 3. In one sentence, what is a "liquid biopsy?" In one sentence, what does a liquid biopsy replace? State 3 advantages and 3 disadvantages of liquid biopsies. (5 marks) 4. In one sentence, what is "radiomics?" (1 mark) Page 4 of 24
5. In radiomics, what is "consensus contouring" and why is it used? (2 marks) 6. In one sentence, what is "precision health?" (1 mark) 7. In one sentence, what is the "exposome?" Give two examples. (2 marks) Page 5of24
8. State the meanings of the following WHMIS symbols. (2 marks) a. b. C. d. e. f. h. 9. In Question 8, the WHMIS symbols are from 1998. Name two changes that were made to WHMIS symbols in 2015. (1 mark) Page 6 of 24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
10. You have just completed an experiment involving cell culture (growing in media) in a flask. During this experiment you also used pipettes and needles. How should you now safely dispose of your cells and additional materials in the IBBME UG Teaching Laboratory? (2 marks) 11. Name four potential routes of entry for a chemical into the human body. Explain how to best protect yourself against each scenario while working in the IBBME UG Teaching Laboratory. (4 marks) Page 7 of 24
12. You have a small biohazard spill on the lab bench in the IBBME UG Teaching Laboratory. How do you clean this up? (1 mark) 13. Which of the following should be always be disposed of in the yellow waste bin when working in the IBBME UG Teaching Laboratory (1 mark): Gloves Paper towel contaminated with chemical waste Pipet tips, regardless of use Liquid biological waste None of the above 14. When designing primers for qPCR, which of the following criteria should you follow (1 mark): Melting temperature of 65-70°C An amplicon length above 150 b.p. A primer that binds an exon-intron junction A primer length of 18-22 nucleotides A and D Page 8 of 24
15. During the course, you have been using a Class 2 Type B3 biosafety cabinet. a. Match the components of the cabinet as shown in the diagram with the labels provided below. (3 marks) Labels: HEPA filter (exhaust); HEPA filter (supply); Sash; Positive pressure plenum; Negative pressure plenum; Front opening % iA#- \i-w / '. . •. . V an i0i P H Proul kw b. Why do we not allow any strong and vaporous chemicals inside the BSC? (1 mark) Page 9 of 24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
16. Michelle Spence, from the Engineering & Computer Science Library, delivered a lecture on online library resources. In relation to this lecture: a. State what the acronym "CRAAP" stands for, and briefly explain what each element of CRAAP means. (5 marks) b. What is a "false drop?" Give an example of this. (2 marks) Page 10of24
c. What does "P100" stand for? (1 mark) d. How does P100 help formulate a search strategy? (1 mark) Page 11 of 24
17. Darren Kraemer described the product development cycle below in his guest lecture. Based on this lecture, label the following on the timeline below. (7 marks) Clarity on markets & production efficiency Risk Valley of death Costs Investment in large scale production Uncertainty in estimated profits Investment in pilot production Page 12 of 24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
18. "Medical devices" are classified by Health Canada according to their risk level. In the table below, state the Canadian Classification (I to IV) and the Risk Level (High to Lowest) for each medical device. (5 marks) Medical Device Canadian . . Classification Risk Level Glucose monitor Bandage Surgical gloves HIV test kits Contact lenses Culture media Pacemakers Orthopaedic implants I. Diagnostic ultrasound systems j. Reusable surgical scalpel Page 13of24
19. State what kind of clinical trial design describes each following situation. (5 marks) A study in which the participants are grouped primarily according to genetic mutations that may drive their disease, rather than by disease site or histology. A study in which the participants' treatments is allowed to be modified during the study by observing the participants' outcomes during the treatment. A study in which the participants are divided by chance into separate groups that compare different treatments or other interventions. At the time of the trial, it is not known which treatment is best. A study where there is a single participant. A study where participants are assigned to different treatment arms based on their type of cancer and their genetic mutations. 20. Why is human DNA polymerase unsuitable for PCR? (2 marks) Page 14 of 24
21. When analyzing DNA and RNA, what does the 260/280 ratio refer to? Why is this important? What ratio should you aim for when working with DNA? What ratio should you aim for when working with RNA? Limit your answer to each question to a single sentence. (5 marks) 22. Briefly describe the similarities and differences between PCR and qPCR. Describe a situation where PCR would be more suitable than qPCR and vice versa. (5 marks) Page 15of24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
1.0 23. The figure below shows receiver operating characteristic (ROC) curves illustrating the performance of computer-aided detection 1 (CAD1) algorithm (ROC), CAD2 algorithm (ROC2), and a radiologist (ROC3), for the detection of prostate cancer. 01 0.2 0.5 1.0 False Positive Rate (1 - Specificity) How does the performance of radiologist (ROC3) compare to that of CAD1 algorithm (ROC1) for prostate cancer detection? (1 mark) For prostate cancer screening, assuming that the maximum acceptable false positive rate is 20%, which CAD algorithm (CAD1 or CAD2) would perform better and why? (2 marks) Page 16of24
In what scenario would you pick CAD1 algorithm over CAD2 algorithm? (1 marks) In what scenario would you pick CAD2 algorithm over the radiologist? (1 marks) 24. In the attached paper by N. Shen et al., "Steps toward Maturation of Embryonic Stem Cell-Derived Cardiomyocytes by Defined Physical Signals," the authors explored different techniques to assess ESC-CM maturation. Based on this paper, please answer the questions below. a. The bioreactor was designed such that the flow rates of 1.48 to 5.92 mL/min could be tested. What shear stresses over the membrane were expected to be created? Why did the authors select these flow rates? (2 marks) Page 17of24
b. What is "Raman microspectroscopy?" Name one advantage and one disadvantage it can have over other optical methods (e.g., flow cytometry and fluorescence microscopy)? (3 marks) 25. Please answer the following questions based on the attached paper by G. Benton et al., "In Vitro Microtumors Provide a Physiologically Predictive Tool for Breast Cancer Therapeutic Screening." In one sentence, what is the authors' overarching motivation for conducting this study? (1 mark) Did the authors conclude that the in vitro microtumors provided a physiologically predictive tool for breast cancer therapeutic screening? How did they reach that conclusion? (1 mark) Page 18of24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
c. Assuming the author's conclusions are correct, to what extent do you think this study provides a physiologically predictive tool for breast cancer therapeutic screening and why? (2 marks) d. How could the authors separate the cells in their co-culture design so they could look at the effects of drugs on different cell types? (1 mark) 26. Please answer the following questions based on the attached paper by Y. Chen et al., "Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis." a. What is the novel scientific advance do the authors claim to achieve in this paper? (1 mark) Page 19of24
b. What tools do the authors use to assess their system? What was/were the control(s) used and do you think this was sufficient? (2 marks) c. What are some possible limitations of this study? (2 marks) 27. Please answer the following questions based on the attached paper by E. Adeghate et al., "Effect of Electrical Field Stimulation on Insulin and Glucagon Secretion from the Pancreas of Normal and Diabetic Rats." a. What EFS condition did the authors claim was the best? (1 mark) Page 20 of 24
b. What is the expected relationship between insulin and glucagon secretion? Did this study verify this relationship? (2 mark) c. The authors claim that their study shows that electrical field stimulation increased insulin secretion from the normal and diabetic rat pancreas. Do you agree with this conclusion? Why or why not? (2 marks) Page 21 0f24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
-This Page Left Intentionally Blank- Page 22 of 24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
-This Page Left Intentionally Blank- Page 23 of 24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help
-This Page Left Intentionally Blank- Page 24 of 24
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help

Related Documents

F23 Lab 6 SDS-PAGE Protocol.docx
Chapter 32.pdf
Chapter 30.pdf
RQ 5 Test Submission- RQ#5 with Answers – Spring 2023 BIOL 1436 29 ....pdf
Ashley Paulino Chapter 13 Worksheet.docx
Chapter 7 - Unanswer.pdf
Chapter 8 - Unanswer.pdf
Deol_55463780_SpeakerSeries6.pdf
SCIE 113 Speaker Series Worksheet 3.docx
Ashley Paulino Lizard Evolution Virtual Lab Report.docx
Ch4_Quiz_ Environmental Science_ ENVIR-010-101-109038.pdf
Lizard Evolution Virtual Lab Report.docx
Recommended textbooks for you
Text book image
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage
Text book image
3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Text book image
Essentials Health Info Management Principles/Prac...
Health & Nutrition
ISBN:9780357191651
Author:Bowie
Publisher:Cengage
Text book image
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
Text book image
Understanding Health Insurance: A Guide to Billin...
Health & Nutrition
ISBN:9781337679480
Author:GREEN
Publisher:Cengage
SEE MORE TEXTBOOKS
Recommended textbooks for you
Text book image
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage
Text book image
3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Text book image
Essentials Health Info Management Principles/Prac...
Health & Nutrition
ISBN:9780357191651
Author:Bowie
Publisher:Cengage
Text book image
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
Text book image
Understanding Health Insurance: A Guide to Billin...
Health & Nutrition
ISBN:9781337679480
Author:GREEN
Publisher:Cengage
SEE MORE TEXTBOOKS