Lab 4 S22

16. Your Excel data graph for Serial Dilution lab:** 16a. Include a clear and labeled graph of DF (x axis) versus Absorbance (y axis). See box above for expectations and review video tutorial of this Excel work. Do not graph two of your data points: your blank or your Cuvette #1 (DF=1). 1 Q A Multiple Styles W S X 3 E D $ 4 To C R F % 5 I T V U ^ 6 G Y 7 H U 8 J BN S ( 9 K M X

Question: Bacterium is gram positive, bacillus, single, short chain. How to determine the bacterium step by step through biochemical tests? Bacteria pool: B. cereus, B. subtilis, B. megaterium, C. perfringens, M. phlei, L. acidophilus, C. butyricum, C.sporogenes, L. lactis

Question shown in picture about microscope calculations

question below is the reference you can also add the procedure but i need the answer most importantly and use the reference if you need it , draw it please for me to understand better

Please help with this

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Discusses the methods to -1 optimise fluorescent cellular (staining (priority first List the freezing -2 parameters of the slow cooling technique Parameter of verification -3 precedure of freezing 9

9. What relationship exists between molecular weight and the rate of diffusion?

how did you get 104 as the dilution factor? I got 10-2 as the dilution factor for the CNFL plate

with possible single or multi-answer types please provide complete solution fast

Question 4: Starting with a culture that contains 5.0 x 107 CFU/ml, describe a serial dilution scheme that would give a plate with 50 colonies.

Question: Assume two Silver/Silver-Chloride electrodes are interfacing with human tissue using the Direct contact and Gel-filled contact approaches. In one paragraph, describe which method is the better technique to reduce motion artifacts and explain why.

QUESTION: Microbes from a blood culture inoculated with blood drawn from a catheter were determined by the appearance of growth on Mannitol Salts Agar and through other tests to be Staphylococcus epidermidis. It can be concluded that: a.  a bloodstream infection caused by S. epidermidis is occurring in the patient from whom the blood was drawn b.  The bacteria fermented mannitol, changing the color of the growth medium from pink to yellow. c  S. epidermidis is present in the sample as a normal flora contaminant d.  That in order to determine if a bloodstream infection is present, these results must be compared to a blood culture of a sample drawn from a peripheral vein

HC Results 61 - 120 of 3161 for Me. 从Mywcc H Take Test: POBS Remaining Time: 1 hour, 24 minutes, 07 seconds. * Question Completion Status: A concentration gradient exists in a solution when the concentration of solutes in the solution is O a. less than the amount of solvent. O b. greater than the amount of solvent. Oc. the same everywhere. O d. not the same everywhere. QUESTION 38 2.5 points Save. The total magnification of an image is determined by lens used. O a adding the magnification power of the ocular lens and the magnification power of the objective O b. multiplying O c. subtracting O d. dividing QUESTION 39 2.5 points Save An A good scientific experiment is reproducible. What does this mean? O a The analysis of the experiment is correct. O b. Anyone can conduct the same experiment in another lab and expect the same results. O c. The experiment is easy to pull off. O d. The experiment has been done many times before with different results each time. 2.5 points Save Answ…

General Electrophoresis Questions: 1. What makes macromolecules move through the gel in electrophoresis?2. What determines the speed at which macromolecules move through the gel in electrophoresis? In a single gel, why do some move faster than others?3. Why do we use different procedures for DNA and protein electrophoresis?

Activity 13 Urine Culture Inoculating Urine with a calibrated loop   The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures.   PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…

DILUTION COLONY COUNT CFU/mL 1:106 155000   1:107 15500   1:108 1550   1:109 155   Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?

Q/Write differences between the fusion embedding method and evaporation embedding method

2nd picture only

2. Compute for the calibration factor. Show your solution. CF = stage micrometer divisions subtended by om x value of one stage µm division ocular micrometer divisions subtended by stage micrometer (1 su x 10μm) 10 ou CF = CF = 1μm Ocular scale Stage micrometer scale or CF = Computation: (stage units x 10μm) ocular units 60 70 80 90 100 Name of Organism: Organism A Objective Used: HPO Total Magnification: 450x Ocular Micrometer units (violet rods): 10 Length of Organism:

I do not understand the results of this experiment, explain what the results of this experiment are and the purpose Table 2   Absorbance vs CoCl2 concentration   Test Tube Number Cobalt Chloride Concentration    (mol/mL) Absorbance at 510 nm       1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138   7   (Unknown)   0.088

Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn v

what is the most efficient way to solve this dilution word problem?

Answer number 2

Questions: For the first two questions, refer to this photo of a SDS PAGE of ladder and antibody. The lane on the left (1) shows a protein ladder and the lane on the right (2) shows antibody. Question 1a. How many different proteins are in the protein ladder? 1b. Ladder and antibody samples are loaded into different the gel. 1c. The approximate size (in kDa) of "Heavy Chain" is: 1d. The approximate size (in kDa) of "Light Chain" is: on Answer 188 kDa 93- 38- 28- 18- 14 6- 3- 12 -heavy chain -light chain

EXPERIMENT : CELL COUNTING FOR DETERMINING VIABLE CELL CONCENTRATION Objectives: To count adherent and suspension cell using hemocytometer.  To calculate viable cell concentration in a suspension. Procedures: Counting Viable Cells using Hemocytometer/Automated Cell Counter▪ Prepare the hemocytometer slide by cleaning the surface with 70% ethanol carefully (do not scratch the semi-silvered coating). Apply the same step for the coverslip. Wet the edges very lightly and press it down over the grooves and semi- silvered counting area. ▪ Collect 20 μl samples from the cell suspension using a pipettor and transfer it immediately to the edge of the hemocytometer chamber. ▪ Expel the suspension and allow it to be drawn under the coverslip by capillary force. Do not overfill the chamber. Blot off any surplus liquid and place the slide under the microscope. ▪ Alternatively, trypan blue dye can be used to stain cells by mixing an equal volume of trypan blue to a cell suspension (1:1) to…

explain what the results of this experiment are and the purpose Table 2   Absorbance vs CoCl2 concentration   Test Tube Number Cobalt Chloride Concentration    (mol/mL) Absorbance at 510 nm       1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138   7   (Unknown)   0.088