pH level and temperature affects enzyme activity. Enzymes are proteins in living things that speeds up chemical reactions. We used a specific enzyme, catalase which breaks down hydrogen peroxide into water and oxygen. The oxygen released from the chemical reactions creates gas bubbles, also known as foam. By measuring the foam, we are able to determine how active the enzyme is. If there is a big amount of foam produced in the test tubes, there is a lot of enzyme activity. If there is little to no
Abstract Enzymes are proteins known as catalysts—substances that increase the rate of chemical reactions. Within this experiment, an enzyme called catalase was measured in two experiments and observed to determine the time required to make 10 mL of oxygen through a catalyzed breakdown of hydrogen peroxide. For experiment 1, the effect of varying substrate concentration on enzyme kinetics, we tested the hypothesis that the higher the substrate concentration, the slower the reaction will be. In experiment
Enzymes Introduction: An enzyme is substance created by living organisms as a catalyst to create a particular biochemical reaction. Biological catalyst are enzymes, biological catalyst are also compounds that increase the amount of a chemical reaction the reaction being changed. Catalysis takes place when the active site of an enzymes starts to combine with the substrate. Which will form the enzyme-substrate complex. When it’s complete the complex separates into enzymes and product(s). Chemicals
Enzyme Activity Lab Hypothesis: 1] Are enzymes reusable? Yes, enzymes are reusable. 2] Which of the following do you think contain catalase? [Manganese dioxide, chicken meat, apple, carrot, potato, or chicken liver] I think that the manganese dioxide, apple, and carrot contain the catalase. 3] Would grinding the materials affect enzyme activity? I think that grinding the materials will increase enzyme activity. 4] Would temperature affect enzyme activity? I think that
Enzyme Lab Conclusion In this lab three hypotheses were tested. If the catalase Solution is added then, the H2O2 will be broken down into harmless byproducts/ there will be an increase in temp compared to the control, if the boiled catalase is added to the H2O2, then there will be no significant increase in temp compared to the control, and if the catalase with acid is added to the H2O2, then there will also be no significant temperature increase compared to the control. The first hypothesis of
between the amount of substrate and the amount of enzyme in a reaction. In our experiment the reaction rate did not increase, it decreased. Because we reduced the amount of substrate in each mixture but kept the amount of enzyme the same the enzyme cannot react with what is not there. The Yeast (enzyme) was the catalyst in the experiment, and not having the same amount of substrate did not cause the 5 reaction to take place faster, it caused the enzyme to not be used. This is why the reaction rate of
#4: Enzymes was having a fungal amylase and a bacterial amylase which are poured into separate sets of 24 wells, which then someone from the group pours three drops of iodine on each row representing four different temperatures (0 degrees, 40 degrees, 55 degrees, and 75-85degrees) and different times reaching from 0 to 10min. The aim for this research was to see which wells with the mixture of the amylases turned yellow, slightly yellow or black. If it turned yellow that means that the enzyme became
The pH scale operates as a logarithmic function because the value changed 10x or greater. A pH of 3 is 10x more acidic than a substance with a pH of 4. 2. Explain what an activation energy is and how it effects a chemical reaction. How does an enzyme and/or a catalyst change the activation energy? Activation energy is the minimum amount of energy for a reaction to undergo. Only a small amount of collisions between
would allow for a wider range of results and a higher accuracy reading of the ideal conditions for enzyme reactivity.
TITLE: ENZYME CATALYSIS PURPOSE: Measure the effects of changes in catalase concentration, substrate concentration, and salinity on the reaction rates of an enzyme. RESEARCH COMPONENTS: What is being tested-what do you know about enzymes? PRELAB QUESTIONS: Explain how the structure of an enzyme is related to its function/activity. All enzymes are structured to react with unique substrates. and each enzyme has an active site where the substrate bonds to the enzyme. The active site of an enzyme