What are two ways to measure the total amount of all proteins in a purification sample? O 1) Calculating the yield and 2) calculating the purity of a purification. O 1) Measuring absorbance at 280 nm and 2) using an enzymatic assay for your protein of interest. O 1) Quantitating the amount of your protein of interest using binding of a labeled antibody (e.g.. an ELISA) and 2) using a dye-binding assay. O 1) Measuring absorbance at 280 nm and 2) using a dye-binding assay.

Organic Chemistry
8th Edition
ISBN:9781305580350
Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions
Section: Chapter Questions
Problem 20.26P
icon
Related questions
Question
What are two ways to measure the total amount of all proteins in a purification sample?
O 1) Calculating the yield and 2) calculating the purity of a purification.
O 1) Measuring absorbance at 280 nm and 2) using an enzymatic assay for your protein of interest.
O 1) Quantitating the amount of your protein of interest using binding of a labeled antibody (e.g.. an ELISA) and
2) using a dye-binding assay.
O 1) Measuring absorbance at 280 nm and 2) using a dye-binding assay.
Transcribed Image Text:What are two ways to measure the total amount of all proteins in a purification sample? O 1) Calculating the yield and 2) calculating the purity of a purification. O 1) Measuring absorbance at 280 nm and 2) using an enzymatic assay for your protein of interest. O 1) Quantitating the amount of your protein of interest using binding of a labeled antibody (e.g.. an ELISA) and 2) using a dye-binding assay. O 1) Measuring absorbance at 280 nm and 2) using a dye-binding assay.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps

Blurred answer
Knowledge Booster
Analytical Separations
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9781305580350
Author:
William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Publisher:
Cengage Learning