the binding constant and the free energy of binding

Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter22: Proteins
Section: Chapter Questions
Problem 22.99P
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I need help calculating the binding constant and the free energy of binding

Base Pairs – A & C

5 ml each

Temperature, (°C)

25.00

[A], (M)

0.0441955

[C], (M)

0.0441955

[AC], (M)

0.00580452

 

Base Pairs – A & T

5 ml each

Temperature, (°C)

25.00

[A], (M)

0.00163564

[T], (M)

0.00163564

[AT], (M)

0.0483644

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