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25
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- QUESTION 48 Identify the best match between the mutation description and term. a. Synonymous mutation: has the potential to cause large changes in transcription and subsequence amino acid sequence due to reading frameshifts b. Nonsense mutation: causes a drastic change in phenotype because the change causes a premature stop in the amino acid sequence c. Indel: a change in the DNA that changes the codon code from one amino acid to another amino acid d. Missense mutation: results in a change in single nucleotide from a purine to another purine or a pyrimidine to another pyrimidine but does not change the amino acid sequenceQuestion 12 The "natural" function of restriction endonucleases is to A help bacteriophages infect cells. B) regulate gene expression from specific promoters. protect bacterial cells which have them. D) remove chromatin from histones.Transposons in bacterial genomes can carry genes,including those conferring ________ resistance
- QUESTION 25 Select all true statements regarding CRISPR: A. CRISPR stands for Clustered regularly interspaced short palindromic repeats B. CRISPR is a natural defense mechanism in bacteria OC. CRISPR has been adapted by scientists for the laboratory for gene editing purposes. D. CRISPR can resurrect the deadQUESTION 23 You can introduce precise edits into the genome using Crispr/Cas9 and ) A. ligation B. homology directed repair C. T-DNA transformation D. CpG methylation E. nonhomologous end joiningIn the text box below, answer the following questions. Remember to label your answers that correspond to the questions, and attempt answering all of the questions to receive full credit. 1. Use the table of the genetic code to complete the following table. The columns represent transcriptional and translational alignments. Label the 5' and 3' ends of DNA and RNA, as well as the amino (N) and carboxyl (C) ends of the protein. end end DNA double G A helix 3' CA U MRNA transcribed G C A TRNA anticodon Trp Amino acid