Homework Help is Here – Start Your Trial Now!
Science
Chemistry
| This problem can be worked with Equations 18-6 on a calculator or with the spreadsheet in Figure 18-5. Transferrin is the iron-transport protein found in blood. It has a molecular mass of 81 000 and carries two Fe³* ions. Desferrioxamine B is a chelator used to 18-A. treat patients with iron overload (Box 11-1). It has a molecular mass of about 650 and can bind one Fe³*. Desferrioxamine can take iron from many sites within the body and is excreted (with its iron) through the kidneys. Molar absorptivities of these compounds (satu- rated with iron) at two wavelengths are given in the table. Both com- pounds are colorless (no visible absorption) in the absence of iron. E(M' cm-') A(nm) Transferrin Desferrioxamine 428 3 540 2 730 470 4 170 2 290 (a) A solution of transferrin exhibits an absorbance of 0.463 at 470 nm in a 1.000-cm cell. Calculate the concentration of transferrin in mil- ligrams per milliliter and the concentration of bound iron in micro- grams per milliliter. (b) After adding desferrioxamine (which dilutes the sample), the absorbance at 470 nm was 0.424, and the absorbance at 428 nm was 0.401. Calculate the fraction of iron in transferrin and the fraction in desferrioxamine. Remember that transferrin binds two iron atoms and desferrioxamine binds only one.

| This problem can be worked with Equations 18-6 on a calculator or with the spreadsheet in Figure 18-5. Transferrin is the iron-transport protein found in blood. It has a molecular mass of 81 000 and carries two Fe³* ions. Desferrioxamine B is a chelator used to 18-A. treat patients with iron overload (Box 11-1). It has a molecular mass of about 650 and can bind one Fe³*. Desferrioxamine can take iron from many sites within the body and is excreted (with its iron) through the kidneys. Molar absorptivities of these compounds (satu- rated with iron) at two wavelengths are given in the table. Both com- pounds are colorless (no visible absorption) in the absence of iron. E(M' cm-') A(nm) Transferrin Desferrioxamine 428 3 540 2 730 470 4 170 2 290 (a) A solution of transferrin exhibits an absorbance of 0.463 at 470 nm in a 1.000-cm cell. Calculate the concentration of transferrin in mil- ligrams per milliliter and the concentration of bound iron in micro- grams per milliliter. (b) After adding desferrioxamine (which dilutes the sample), the absorbance at 470 nm was 0.424, and the absorbance at 428 nm was 0.401. Calculate the fraction of iron in transferrin and the fraction in desferrioxamine. Remember that transferrin binds two iron atoms and desferrioxamine binds only one.

Introduction to General, Organic and Biochemistry
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter22: Proteins
Section: Chapter Questions
Problem 22.65P: 22-65 (a) What is the difference in the quaternary structure between fetal hemoglobin and adult...
See similar textbooks
icon
Related questions
Question
This problem can be worked with Equations 18-6 on a calculator or with the spreadsheet in Figure 18-5. Transferrin is the iron-transport protein found in blood. It has a molecular mass of 81 000 and carries two Fe³* ions. Desferrioxamine B is a chelator used to 18-A. treat patients with iron overload (Box 11-1). It has a molecular mass of about 650 and can bind one Fe³+. Desferrioxamine can take iron from many sites within the body and is excreted (with its iron) through the kidneys. Molar absorptivities of these compounds (satu- rated with iron) at two wavelengths are given in the table. Both com- pounds are colorless (no visible absorption) in the absence of iron. e(M¯' cm¯') A(nm) Transferrin Desferrioxamine 428 3 540 2 730 470 4 170 2 290 (a) A solution of transferrin exhibits an absorbance of 0.463 at 470 nm in a 1.000-cm cell. Calculate the concentration of transferrin in mil- ligrams per milliliter and the concentration of bound iron in micro- grams per milliliter. (b) After adding desferrioxamine (which dilutes the sample), the absorbance at 470 nm was 0.424, and the absorbance at 428 nm was 0.401. Calculate the fraction of iron in transferrin and the fraction in desferrioxamine. Remember that transferrin binds two iron atoms and desferrioxamine binds only one.
Transcribed Image Text:This problem can be worked with Equations 18-6 on a calculator or with the spreadsheet in Figure 18-5. Transferrin is the iron-transport protein found in blood. It has a molecular mass of 81 000 and carries two Fe³* ions. Desferrioxamine B is a chelator used to 18-A. treat patients with iron overload (Box 11-1). It has a molecular mass of about 650 and can bind one Fe³+. Desferrioxamine can take iron from many sites within the body and is excreted (with its iron) through the kidneys. Molar absorptivities of these compounds (satu- rated with iron) at two wavelengths are given in the table. Both com- pounds are colorless (no visible absorption) in the absence of iron. e(M¯' cm¯') A(nm) Transferrin Desferrioxamine 428 3 540 2 730 470 4 170 2 290 (a) A solution of transferrin exhibits an absorbance of 0.463 at 470 nm in a 1.000-cm cell. Calculate the concentration of transferrin in mil- ligrams per milliliter and the concentration of bound iron in micro- grams per milliliter. (b) After adding desferrioxamine (which dilutes the sample), the absorbance at 470 nm was 0.424, and the absorbance at 428 nm was 0.401. Calculate the fraction of iron in transferrin and the fraction in desferrioxamine. Remember that transferrin binds two iron atoms and desferrioxamine binds only one.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Spectroanalytical Methods
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Introduction to General, Organic and Biochemistry
Introduction to General, Organic and Biochemistry
Chemistry
ISBN:
9781285869759
Author:
Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:
Cengage Learning
Chemistry for Today: General, Organic, and Bioche…
Chemistry for Today: General, Organic, and Bioche…
Chemistry
ISBN:
9781305960060
Author:
Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:
Cengage Learning
SEE MORE TEXTBOOKS