QUESTION 1Have a look at the plasmid map of PGEX-6P-1-InIA on page 11 of your prac guide. If you perform a restriction digestion onPGEX-6P-1-InlA with BamHI and Not/ in order to cut out the In/A functional domain; what size will the two bands be if thedigestion is run on an agarose gel?A. 1380 and 4967B. 1393 and 4954C. 2339 and 4008D. 2339 and 4954 Apol (5322)Apal (5258)laclApoI (4612)tac promoterpGEX-6P-1-INIA6347 bpPstI (3301)AmpicillinGSTC3 cleavage siteApal (941)Bam HI (946)Pst I (1091)Pst I (1412)inlA funct domPst I (1937)NotI (2339)

Restriction enzymes are enzymes that cleave DNA sequences at specific sites. Due to this ability,…

Answered: Have a look at the plasmid map of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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BM4_DNA AND PROTEIN S X /1FAIPQLSDP_g5B-629FSHNpGnTMIEppLS4A71zBd4vcUBqNUILubXONw/formResponse 4. What is the nitrogen base pair of Adenine in transcription? O Cytosine O Uracil O guanine O thymine 5. The central dogma of Molecular Biology states that There are four nitrogen bases in DNA, two purines (adenine and guanine) and two pyrimidines (cytosine and thymine). Which process is not included in the central dogma? duplication transcription translation O translocation Leadple
why is the tas2r38 gene also called the ptc gene
e B 1_30*_SP23 - General Biology I (for majors)/11364 of € 2 A us page X F1 What conditions would we find on the gene of a prokaryote if there is low amounts of tryptophan within the cytoplasm? Select one: a. lactose would attach to the repressor removing it from the promoter promoting the transcription of lactase b. no repressor is on the promoter creating constant transcription of lactase c. tryptophan would attach to the repressor removing it from the promoter promoting the transcription of lactase O d. repressors would bind to the promoter stopping the transcription of lactase e. lactose would attach to the repressor removing it from the promoter promoting the transcription of tryptophan producing enzymes no repressor is on the promoter creating constant transcription of tryptophan producing enzymes tryptophan would attach to the repressor binding it to the promoter and stopping the transcription of tryptophan producing enzymes O f. O g. 2 Oh. tryptophan would attach to the…
Each year in the United States, there are over 230,000 newcases of prostate cancer and almost 28,000 deaths. A 3.8-Mbregion on chromosome 8 (8q24), called a gene desert, has genes but contains enhancer sequences that potentiallyconfer significant risks for prostate cancer. One particular enhancerallele, which is known to be associated with an elevated risk forprostate cancer, physically interacts with the promoter region ofthe nearby MYC gene and facilitates its upregulation. Overexpressionof MYC, which encodes a cell-cycle regulatory protein, isobserved in multiple types of cancer (see Chapter 24). The riskallele has a frequency of 49 percent in men of European descentand 81 percent in men of African ancestry. Most of the differentialMYC activity associated with the risk allele occurs during prenataldevelopment, raising the possibility that testing for this alleleearly in life can be used to identify those in the African-Americanpopulation who are at very high risk for prostate…
BclI cleaves after the first T: 5’ T GATCA 3’ 3’ ACTAG T 5’ Does cleavage by BclI result in a 5’ or 3’ overhang? What is the sequence of this overhang?
Transforming an Animal In order to create the transgenic cow, your lab first needs to create a DNA vector containing the insulin gene. This step involves a considerable amount of scientific terminology. Make sure you understand the meaning of key terms. Match the following terms with their correct definitions. | ampicillin resistance gene 5 restriction site 6 Origin of replication 7 Ligase 2 promoter 3 Xhol Ч ехоn is a region of DNA that is not transcribed. is the location in the plasmid that is recognized by the restriction enzyme Xhol. is an enzyme that joins DNA fragments together. is the location on the plasmid where DNA replication begins. is a region of DNA that initiates transcription of a gene. is an restriction enzyme that looks for the sequence TCGA. is a gene that enables you to identify bacterial cells that have taken up the plasmid.
Identify the ligand that bind to uniprotkb-P39086, the type of allostery, and describe the general function of the protein
Certain hormones, such as epinephrine, can increase the levels ofcAMP within cells. Let’s suppose you pretreat cells with or withoutepinephrine and then prepare a cell extract that contains theCREB protein.You then use an electrophoretic mobility shift assay to analyzethe ability of the CREB protein to bind to a DNA fragmentcontaining a cAMP response element (CRE). Describe what theexpected results would be.
Anti apoptosome anti N anti protein X anti caspase based on thé data above where is the WT protein actually cleaved D380 D380 and D835 D835 D835 and D840 D840 all are cleaved none are cleaved D380 and D840 if you compared the size of WT and the D380E mutant before apoptosis on a western blot how would they appear? D380E would be larger they would be indistinguishable WT would be larger how many amino acids long is the larger band in the lane labeled D380E/D835E
In addition to Tc1, the C. elegans genome contains otherfamilies of DNA transposons such as Tc2, Tc3, Tc4, andTc5. Like Tc1, their transposition is repressed in thegerm line but not in somatic cells. Predict the behaviorof these elements in the mutant strains where Tc1 is nolonger repressed due to mutations in the RNAi pathway.Justify your answer.
Propose a mutation for Uniprotkb-P39086 and it’s effect
Xist gene is located in a 450 kb region of DNA called the X inactivation center (XIC) and is transcribed only from the future inactive X chromosome. The Xist noncoding RNA (ncRNA) triggers X inactivation by coating the X chromosome that produces it and by recruiting enzymes that mediate Barr body formation. How one X chromosome is chosen to express Xist is unknown. Active X • Histone modifying proteins ~Xist ncRNA XIC O Histone methyltransferase (HMT) Histone acetyl transferase (HAT) Histone deacetylase (HDAC) DNA methyltransferase (DNMT) Inactive X Xist Į Which of these enzymes is unlikely to be recruited by Xist?

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