Glucose 1-phosphate = glucose 6-phosphate 4.5 х 10-3 м 9.6 x 10-2 M
Q: Phosphoglycerate-mutase catalyses the isomerization of 3-phospho-glycerate to 2-phospho-glycerate.…
A: Kcat is defines as a no of moles of substrate transformed per minute per mole of active subunit or…
Q: Calculate kcat for an enzyme where Vmax is 12 U s. mL and the total enzyme concentration is 0.016 U…
A: Given Values: Vmax = 12 u s-1ml-1 Total enzyme concentration = 0.016 U ml-1
Q: Given the following information: 1) Glucose-6-Phosphate → 2) culate BOTH AG! AND K'eq for the…
A: Gibbs free energy is the amount of energy, which is used to perform several other functions.
Q: Assume that you have a solution of 0.1 M glucose 6-phosphate. To this solu- tion you add the enzyme…
A: An enzyme is a substance that acts as a catalyst in living organisms, regulating the rate at which…
Q: value of AG" for the conversion of 3-phosphoglycerate to 2-phosphoglycerate (2PG) is +4.40 kJ/mol.…
A: dGo = -RTlnKeq T = 25 + 273 = 298 K Keq = [2-phosphoglycerate]/[3phosphoglycerate]
Q: dihydroxyacetone phosphate --> glyceraldehyde-3-phosphate AGO' = -7.90 kJ/mol a. Calculate the…
A: The reactions of glycolysis are intimately linked to release of energy in biological system and…
Q: . For parts (a) and (b) of this problem use the following standard reduction potentials, free…
A: The equilibrium constant of a chemical reaction is the value of its reaction quotient at chemical…
Q: 1x10exp-7 From the following enzyme-catalyzed kinetic data, estimate K, and V, 'm m 25 20 15 10 1…
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: In a major metabolic pathway involving the monosaccharide glucose, one of the reactions involve the…
A: The given reaction is a part of the glycolysis pathway. During glycolysis, glucose is first…
Q: G6P(ag) 2 F6P(ag) AGº=+1.7kJ/mol at 25 °C Consider the reaction: Estimate the fraction of F6P…
A: The conversion of glucose 6 phosphate to fructose 6 phosphate is the second step of glycolysis. The…
Q: 1. Penicillin is hydrolyzed and thereby rendered inactive penicillinase, an enzyme present in some…
A:
Q: A catalyst like lactase that assists in the metabolism od milk is best described as Group of answer…
A: Note: We are authorized to answer one question at a time since you have not mentioned which question…
Q: A) Is this reaction ( in picture provided) in equilibrium? B) If it is not then ,what is ∆G' at…
A: Gibbs free energy change, equilibrium constant and reaction quotients are values for a reaction…
Q: Table 1 shows the kinetic data that have been obtained for glucoamylase from Aspergillus niger at…
A: Enzymes catalyse the biochemical reactions by decreasing the activation energy. Enzyme kinetics are…
Q: Consider the reaction: Fructose-1,6-bisphosphate (F1,6BP) +→ Glyceraldehyde 3-phosphate (GAP) +…
A: All cells require energy in the form of ATP to carry out a variety of functions, such as active…
Q: The following were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics:…
A: Enzymes are proteins which accelerate the rate of biochemical reactions. The Michaelis-Menten plot…
Q: For the series of reactions below, what is the overall reaction and the value of ∆Go’(kJ/mol) for…
A: Consider a set of reactions 1 and 2; Reaction 1 : a→b have a ∆G0' = y Reaction 2 : c→d have a ∆G0' =…
Q: Consider the binding reaction L + R → LR, where L is a ligand and R is its receptor. When 1 × 10−3 M…
A: The equilibrium constant of a chemical reaction is the value of its reaction quotient at chemical…
Q: 62. How do you calculate ATP in beta oxidation of even numbered fatty acid? * The formula (7 x C)-6…
A: β oxidation is the process of degradation of fatty acids to form acetyl CoA and energy in the form…
Q: The enzyme lysozyme kills certain bacteria by attacking a sugar called N-acetylglucosamine (NAG) in…
A: Enzymes are proteinaceous substances which are capable of catalyzing chemical reactions of…
Q: If 0.1 M glucose 1-phosphate is incubated with phosphoglucomutase, the glucose 1- phosphate is…
A: Answer to (i) Given, concentration of glucose-1-phosphate at equilibrium = 4.5 x 10-3 M And…
Q: The rate constant for the uncatalyzed reaction of two molecules of glycine ethyl ester to form…
A: Given values: Rate constant in the uncatalyzed reaction = 0.6 M-1s-1 In the presence of catalyst…
Q: 4. The standard free energy change for the reaction catalyzed by phosphoglucomutase is -7.1 kJ/mol.…
A: 4. Enzymes function as biological catalysts as they increase the rate of reactions by lowing…
Q: In a 250ul reaction containing 0.3 nmol of a Michaelis enzyme with Km = 4.4 x 10 M and saturating…
A: Given, Vo= 6.3 * 10-3 M min-1 Km= 4.4 * 10-5 M [S]= 1 (Because its given that the condition is…
Q: Neutral sphingomyelinase 2 converts sphingomyelin into ceramide and phosphorcholine. What kind of…
A: Neutral sphingomyelinase 2 (nSMase2) helps in breakdown of sphingomyelin to ceramide and…
Q: Inside cells, the AG value for the hydrolysis of ATP to ADP + Pi is approximately -50 kJ/mol (-12…
A: Gibbs free energy refers to amount of free energy (G) present. In respect to chemical reactions,…
Q: Begining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate…
A: The reaction given in the problem is the reaction between Pyruvate to Lactate conversion which is…
Q: The ΔG°′ for hydrolytically removing a phosphoryl group from ATP is about twice as large as the ΔG°′…
A: Adenosine triphosphate is an organic compound and hydrotrope that provides energy to drive many…
Q: [S] (MM) 0.5 1.0 1.5 Vo No Inhibitor (mM min-¹) Vo With Inhibitor (mM min¹¹) 16.67 25.25 30.49 23.5…
A: Enzymes are protein molecules that increase the rate of reactions by decreasing the activation…
Q: The decomposition reaction of atmospheric peroxyacetylnitrate (PAN) to products was studied, and the…
A: Multiple subparts asked. I will answer the initial 3 subparts, as allowed by guidelines. Arrhenius…
Q: Consider the following equilibrium at 25ºC :Glucose-1-Phosphate Glucose-6-PhophateUsing the…
A: The change in Gibbs free energy ∆G0 is a thermodynamics potential that is used to calculate the free…
Q: 28. What is the intracellular glucose concentration if the AG for the following reaction is -20.1…
A: The reaction given in the question is Glucose-6-Phosphat -> Glucose + Pi Delta G = -20.1 kJ/mol…
Q: 9. If 32p-labeled inorganic phosphate were introduced to erythrocytes undergoing glycolysis which of…
A: Glycolysis is the process of conversion of one molecule of pyruvate into two molecules of pyruvate.…
Q: For the following calculations, please provide your answers in whole numbers (no decimals). A…
A: Enzymes are biocatalysts which increase the rate of chemical reaction by decreasing the activation…
Q: You have obtained experimental kinetic data for two versions of the same enzyme, a wild‑type and a…
A: Michaelis-Mention constant (Km) or equilibrium constant for the dissociation of the E-S complex is…
Q: While furthering your studies of iocane powder you determine that a ceular enzyme, iocase will…
A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…
Q: (i) a) Construct an empty table with the following column headings: Substrate concentration [S] and…
A: Enzymes are usually protein molecules and catalyzes biochemical reactions without being consumed in…
Q: Given the following reagents, give the moles of each component (acid & base). 2. What are the…
A: "Since you have asked multiple questions, we will solve the first two question for you. If you want…
Q: Given that the standard free-energy change for the reaction glucose + Pi →glucose 6-phosphate is…
A: Given, ∆G°' for glucose + Pi → glucose 6-phosphate is 13.8 kJ/mol.∆G°' for ATP → ADP + Pi is −30.5…
Q: Calculate AG for the below balanced redox reaction as written. You may find a list of standard…
A: I2 + 2e- gives 2I-1 Standard potential is 0.54 V(E° cathode) Al gives Al3+ + 3e- Standard potential…
Q: Phosphoglycerate-mutase catalyses the isomerization of 3-phospho-glycerate to 2-phospho-glycerate.…
A: Enzymes are biological catalysts that aid in the speeding up of chemical reactions. Proteins called…
Q: 1. Enzyme X has a molecular weight of 35,000 Da. When this enzyme is assayed for enzyme activity,…
A: Enzyme kinetics is used to study the rates of enzyme catalysed biochemical reactions. In this study…
Q: Phosphoglycerate-mutase catalyses the isomerization of 3-phospho-glycerate to 2-phospho-glycerate.…
A:
Q: A bacterial enzyme catalyzes the hydrolysis of maltose as shown in the reaction given below:…
A: the enzyme kinectics is governed entirely by one equation, the Michaelis-Menten Equation. the…
Q: If 32P labeled (radioactive phosphate) inorganic phosphate were introduced to erythrocytes…
A: Glycolysis converts glucose into pyruvate or lactate with the generation of energy. The process of…
If a 0.1 M solution of glucose 1- phosphate at 25 °C is incubated with a catalytic amount of phosphoglucomutase, the glucose 1-phosphate is transformed to glucose 6-phosphate. At equilibrium, the concentrations of the reaction components are
Calculate Keq and ΔG′° for this reaction.
Step by step
Solved in 3 steps
- Materials - 2021FA-CHM-1O X Bb 4427577 d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig 6 / 9 100% Exercise 1: Standard Curve for Protein Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and spectrophotometer: following measurements were obtained using a Protein Concentration (mg/ml) Absorbance (A) 0.26 0.098 0.56 0.213 0.383 0.84 1.12 0.473 1.40 0.527 TASKS: 1. Enter the data into Excel - the…6-26. Given the following equilibria, calculate the concentration of each zinc species in a solution saturated with Zn(OH),(s) and containing [OH ] at a fixed concentration of 3.2 x 10-7 M. Zn(OH),(s) Kp = 3 × 10 16 Zn(OH) B1 = 1 × 104 Zn(OH),(aq) B2 = 2 × 1010 Zn(OH), Zn(OH); B3 = 8 × 1013 2- ВA — 3х 101580mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
- 6:38 71.0 KB/S 90 Assign B.JPG Assignment B Polysaccharides 1. What is the difference between the structure of cellulose and the structure of amylose? 2. How does the structure of amylose differ from that of amylopectin and glycogen? 3. What is the major physiological purpose of glycogen? 4. Where in the body do you find glycogen stored? 5. Where are a-amylase and ß-amylase produced? 6. Where do a-amylase and ß-amylase carry out their enzymatic functions?Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7 Fibrinogen 5.8 340 Y-globulin 6.6 160 Collagen Hemoglobin (monomer) 6.6 115 7.1 16 Myoglobin 7.0 16.7 From the given table, draw the appearance after visualization of a protein mixture containing the seven proteins in the table when subjected to SDS-PAGE.Acetocholinesterase is an enzyme possessing a single active site that metabolizesacetylcholine with a turn over number of 1.4 x 10^4s-1. How many grams of acetylcholine(molecular formula C7NO2H16+) will 2.16 x 10^-6 g acetocholinesterase metabolize in 60minutes? (The enzyme’s molecular mass is 4.2 x 10^4 g/mol).
- 1N(10 M min'y1 3. To the right is a Lineweaver-Burk plot for enzyme X along with two additional (A) 501 lines from testing a low and high concentration of a new inhibitor. a. Mark which line is the enzyme with no inhibitor, low concentration of inhibitor and high concentration of inhibitor. b. What is the KM for this enzyme? -50 50 150 What is the Vmax for this enzyme? 1/S (M-1) С. d. What type of inhibition occurs in the presence of the new inhibitor? -25C17H29COOH linolenic acid non-saponifiable ω-3 fatty acid All are correctThe tripeptide abbreviated as Ala-Ser-Val is the same as the tripeptide abbreviated as Val- Ser-Ala. O True O False
- Given this: Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7 Fibrinogen 5.8 340 Y-globulin 6.6 160 Collagen 6.6 115 Hemoglobin (monomer) 7.1 16 Мyoglobin 7.0 16.7 What is the appearance after visualization of a protein mixture containing the seven proteins above when subjected to SDS-PAGE?Given this: Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7 Fibrinogen 5.8 340 Y-globulin 6.6 160 Collagen 6.6 115 Hemoglobin (monomer) 7.1 16 Myoglobin 7.0 16.7 Does all proteins separated properly w/ isoelectric focusing?calculate the pI for tripeptide R-H-D