Genomic sequencing cannot be used to: Predict a protein coding gene Predict structure and function of a protein encoded in a gene Locate similar sequences (sequence homology) Locate repetitive sequences Predicting alternative splicing patterns
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- b) A gene was isolated from mouse and sequenced by a research group. When the sequencing result was obtained, the research leader requested her assistant to generate the results below (Figure 1) using a Bioinformatics tool. >>> Descriptions Graphic Summary Sequences producing significant alignments select all 200 sequences selected Alignments Taxonomy Mus musculus itin 1(Sit1), transcript variant 1. mRNA Mus musculus Siapha(S)RNA completed Mus musculus Sic2alpha orain IS2alphai mRNA complete ods PREDICTED: Mus.caroll situin 1(Sirt1) transcript variant X1. mRNA ✔Mus musculus situin 1(S1), transcript variant 2.mRNA ✔PREDICTED: Anvicanthis niloticus situin 1 (Sirt1) transcript variant X1 mRNA PREDICTED: Acanthis nicticus siruin 1.(Sirt1) transvaatX2 NA (i) Description (ii) Download ✔Mus musculus mutent stem cell CRL-2070 NE cDNA. RIKEN M-length enriched library, clone:G430107915 productsituin 10sent: 0030 6805 95% PREDICTED:Mastommys.coucha sittuin 1 (Sirt1), transcript variant X1. mRNA…A portion of the coding sequence of a cloned gene is shown here:5΄–GCCCCCGATCTACATCATTACGGCGAT–3΄3΄–CGGGGGCTAGATGTAGTAATGCCGCTA–5΄This portion of the gene encodes a polypeptide with the aminoacid sequence alanine–proline–aspartic acid–leucine–histidine–histidine–tyrosine–glycine–aspartic acid. Using the method ofsite- directed mutagenesis, a researcher wants to change the leucinecodon into an arginine codon, using an oligonucleotide that is19 nucleotides long. What is the sequence of the oligonucleotidethat should be used? Designate the 5′ and 3′ ends of the oligonucleotidein your answer. Note: The mismatch should be in the middleof the oligonucleotide, and a 1-base mismatch is preferableover a 2- or 3-base mismatch. Use the bottom strand as the templatestrand for this site-directed mutagenesis experiment.You have assembled a new prokaryotic genome and are annotating the genes. If you were to search for highly conserved sequences necessary for normal gene expression, what type of annotation are you doing? Group of answer choices ad hoc annotation homology based annotation preliminary annotation ab initio annotation
- Place the steps necessary to perform RNA-Seq in the correct order. Drag the text blocks below into their correct order. Reset MAAAAAAAAAKK MAAAAAAAAAAM Compare sequences to known genome sequence. Create cDNA using reverse transcription with primers complementary to linkers. Attach linkers to the ends of the RNAs. Perform next-generation DNA sequencing. Isolate RNA from cells or tissues of interest. Fragment the RNAs.Can Bioinformatics be used for sequence annotation to identifyprotein-coding and noncoding sequences of a gene ? If yes then explain how ?This descriptor refers to the highest alignment score of all matched sequences to the sequence placed in the BLASTn search. Query cover Maximum score and Total score E-value Ident Accession number
- What is meant by the term "alignment" in genomic research and in what ways does alignment aid sequence assembly? How can relying on alignment lead to potential errors?What is a database? What types of information are stored within adatabase? Where does the information come from? Discuss theobjectives of a genome database.A protein has the following amino acid sequence: Met-Tyr-Asn-Val-Arg-Val-Tyr-Lys-Ala-Lys-Trp-Leu-Ile-His-Thr-Pro You wish to make a set of probes to screen a cDNA library for the sequence that encodes this protein. Your probes should be at least 18 nucleotides in length. Q. Which amino acids in the protein should be used to construct the probes so that the least degeneracy results?
- Annotation involves identifying genes and gene-regulatory sequences in a genome. List and describe characteristics of a genome that are hallmarks for identifying genes in an unknown sequence. What characteristics would you look for in a bacterial genome? A eukaryotic genome?What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.List down and briefly describe three other sequence alignment tools other than BLAST used in Bioinformatics.