Exercise 10 & 11:Carbohydrate catabolism in general - primarily looking at fermentation paths in these exercisesExoenzymes & Amylase - only specific enzyme for this exerciseStarch agar (what looks like for positive and negative, what enzyme you are testing for, how to do test - mustadd iodine reagent before reading!)Sugar Fermentation tubes:what testing for (acid or acid/gas production), observe and interpret results completely (A = acid, Ag =Acid + gas, K= alkaline) [Acid = yellow; K= red}MRVP broth - do twice, once for MR methyl red pathway and one for VP test Voges Proskauer pathwayHow to do both tests, what is each testing for, observe and interpret results completely, what reagentsare addedMR (mixed acid fermentation)VP (2,3 butanediol fermentation)Citrate slant (citrate sole carbon source)TSI (glucose/lactose/sucrose fermentation to acids, H₂S production)observe and interpret results completely like A/A, K/K, A/H2S, etc.what processes are occurring in butt vs slantfor slant sugar reactions - recall, in slant, you cannot distinguish if one or the other sugar present(lactose and or sucrose) has been fermented to acids, only that at least one of them has beenfermented to acids

Catabolism and anabolism are two fundamental processes involved in the metabolism of organic…

Answered: Exercise 10 & 11: Carbohydrate…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 22P: Based on your residing of this chapter, what would you expect to be the most immediate effect oil...

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Activity 13.2. Label the steps of Krebs cycle. NADH Krebs cycle ADP FADH,
Test Tube # Enzyme 1 (uL added) Product Formation (umol) 1 0 0 2 25 0.08 3 50 0.15 4 75 0.22 5 100 0.3 6 125 0.37 7 150 0.45 8 175 0.53 9 200 0.59 10 225 0.68 11 250 0.74 A. Make a graph is Excel/Google Sheets plotting Product formation (umol) on the y-axis and Enzyme volume added on the x-axis. Paste the graph below. Make sure to have axis labels and a title on the graph. B. What can you conclude from this graph, ie how is the amount of product formed changing as a function of the amount of enzyme present?
3:43 ll LTE O moellim.riphah.edu.pk Biochemistry-l Dashboard / My courses / Biochemistry-II | General / QUIZ 1 Question 7 Not yet answered Marked out of 1.00 P Flag question ADP will the regulatory enzymes of TCA cycle? Select one: O a. None O b. Both depending on condition O c. Inhibit O d. Activate Next page Course Outline
Case Study: Case Study: Catalase Activity Catalase H,O, 0 + O2 (2) Catalase is an enzyme that converts hydrogen peroxide (H,O) to oxygen and water. An experiment investigated the effect of temperature on the rate of the catalase reaction Small (10 cm) test tubes were used for the reactions, each containing 0.5 cm of enzyme and 4 cm of hydrogen peroxide. Reaction rates were assessed at four temperatures (10°C, 20°C, 30'C. and 60 C). For each temperature, there were two reaction tubes (e.g. tubes 1 and 2 were both kept at 10°C). The height of oxygen bubbles present after one minute of 10°C 20°C 30°C 60°C Height of охудen bubbles 4 cm H,02+ 0.5 cm erzyme reaction was used as a measure of the reaction rate: a laster reaction rate produced more bubbles. The entire experiment, involving eight tubes, was repeated on two separate days. Tubes Tubes Tubes 1 & 2 Tubes 3 & 4 5 & 6 7 & 8 1. What is the purpose of this experiment? inrestrgatee effect of demperalure on the rate ot he 2. Write a…
CELLULAR RESPIRATION AND FERMENTATION Name: Date: Instructor: Section/Group: POST-LAB QUESTIONS 1. Both cellular respiration and fermentation ordinarily begin with which molecule? ves 2. What role does cellular respiration play in the metabolism of an organism? 3. Glucose breakdown results in the breaking of C-H bonds and stored energy is released. Compare and contrast the end products of fermentation and cellular respiration in terms of their energy produced. 4. Fermentation results in the net production of only 2 ATP, while cellular respiration results in the production of at least 36 ATP. Explain these results with reference to the end products of both of these processes. Cellular Respiration and Fermentation 93
Instructions: 1.provide a solution using a table, listing all the specific individual reactions that will generate ATP either by substrate level phosphorylation (SLP) or by oxidative phosphorylation (OP). 2. Shall the given question require a shuttle system, please use both and indicate the net ATP for each. 3. Get the net ATP for each question given you Specific reaction Number of ATP (+ or -) Question 1: fructose 6-phosphate to 6C02
Name: Sincere Frias Date: (0/2/2od0 Period Ced th nzy ck. Enzyme Models & Factors Affecting Enzyme Action PART I – Lock and Key Model st In this part you will construct models of enzymes. Remove the last page and cut all shapes out. 2. The different shapes will represent both enzymes and substrates. 3. Match up as many of the pieces as you can. 1. Questions 1. The enzyme maltase combines the substrates h ydrolysis + Starch molecule to produce the disaccharide maltose. 2. The enzyme ATPase combines the substrates_molecule and to produce the ATP (Adenosine TriPhospate). Iriglyceride 3. The enzyme lipase works on the substrate called 4. The enzyme phosphatase works on the substrate called 5. What is the relationship between the substrate and the enzyme?
QUESTION 54 Classification. Use the five compounds listed below for the following questions. a. Ethanol This compound is utilized in alcoholic fermentation and lactate - vfermentation. b. Pyruvate c. Lactate This compound is produced by fast-twitch muscle fibers. d. Carbon dioxide e. Acetaldehyde
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Activity 13.5. Arrange the following enzymes of glycolysis. Hexokinase, enolase, aldolase, Pyruvate kinase, phosphofructokinase, phosphoglucoisomerase, phosphoglycerokinase, triose phosphate dehydrogenase, Isomerase, Phosphoglyceromutase, 1. 6. 2. 7. 3. 8. 4. 9. 5. 10.
Challenge question 1: Calculate the number of ATP that will be CH. H-C-coo produced if a microorganism makes use of the following as substrate: HO-C-H socitrate coo NAD ase NADH co, • Lactate Coo glutarate CH, • Succinate čoo NAD COA • Malate

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