How do the following factors affect the activity of restriction enzymes? pH conditions Mg2+ concentrations
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How do the following factors affect the activity of restriction enzymes?
- pH conditions
- Mg2+ concentrations
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- in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of CentrifugationTRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?In bacterial transformation, What is the purpose of ampicillin and arabinose in the growth media?
- All of the following are true about Restriction endonucleases except fashiona)List and describe the purpose of each component of the restriction enzyme digest. b)Why do some tubes have no restriction enzyme?What are the reagents found in a restriction enzyme digestion buffer? What are the roles of these reagents?
- Please determine the relative location of the three restriction enzymes. Enzyme Number of fragments Size (kb) Xbal 2 17, 21 Xhol 2. 5, 33 Xbal/Xhol 5, 12, 21 Kpnl 6, 7, 58 Xbal/Kpnl 6, 7, 8, 17The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.What is the role of the following in the alkaline plasmid screen? G buffer (Cell Suspension Solution) Denaturing Solution (Cell Lysis Solution) Neutralization Solution
- Which of the following parameters should be altered to increase the amount of vector-insert constructs compared to other ligation products? vector:insert ratio o type of vector O incubation temperature O incubation timeYou are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?1 2. 3. Two different cultures of Escherichia coli bacteria were inoculated onto nutrient agar plates. One culture was transformed with pGLO plasmid and the other culture was not. All of the plates shown above are illuminated with ultraviolet light. • Which plate was inoculated with E.coli+PGLO bacteria and included ampicillin antibiotic but did not include arabinose? • Which plate was inoculated with E.coli+pGLO bacteria and included both ampicillin antibiotic and arabinose. • What would you expect to observe on a plate that included ampicillin and was inoculated with E. coli minus (without) PGLO plasmid?