Question 3: Look carefully at the structures of the two molecules shown below and answer thefollowing questions.O=ddCTPO=OHO11HO-P-O-P-O-P-OIOH OHNH₂ddCMPO11HO-P-OOHNH₂A) What would you expect to happen if you added ddCTP to a DNA synthesis reaction in vitroin large excess over the concentration of dCTP?B) What would happen if ddCTP were added at 10% of the concentration of dCTP?C) What effect would you expect if ddCMP were added to the synthesis in large excess overdCTP? At 10% of the concentration of dCTP?

ddCTP is 2',3'-Dideoxycytidine-5'-triphosphate. It does not contain a 3’OH group in the deoxyribose…

Answered: A) What would you expect to happen if…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 10. Which statement on the migration of DNA fragments through agarose gels is false A) Small fragments migrate faster than larger fragments because they can move faster through the agarose pores. B) Large fragments migrate faster than small fragments because they carry more negative charges. C) DNA fragments migrate towards the positive pole. D) Supercoiled DNA may migrate significantly different through the gel than linear DNA of equal size. E) The higher the agarose concentration the better the separation of smaller fragments as compared to larger fragments.
11:29 Protein 6-10092015113530.pdf https:api.schoology.comv1attachment169963838... Name Class Date 2. How are enzymes involved in this process? 3. hаppens anzips"? 4. Why is it important that exact copies of DNA be made? 5. Suppose that a sequence of one DNA strand is T-A-C-A-A-C-G-T-G. What is the corresponding sequence on the other strand? E Concept Mapping The construction of and theory behind concept mapping are discussed on pages vil-ix in the front of this Study Guide. Read those pages carefully. Then consider the concepts presented in Section 7-1 and how you would organize them into a concept i page 74. Notice that the concept map has been started for you. Add the key Now look at the concept map for Chapter 7 on concepts you are important Secti When you have finished the chapter, you will have a completed concept map. 69 1 of 1
Question:- A mixed copolymer was synthesized using 2 parts Cytosine and 1 part Adenine and the resulting mRNA was used for translation. What is the probability of producing the following amino acids? NOTE: Answer in fractions only. 1. Threonine _____ 2. Asparagine _____ 3. Histidine _____ 4. Lysine _____ 5. Proline _____
Question 4. What is the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3' ? b) Assuming that the % GC is = 60%. O (2/10)4 (3/10)² O (3/10)4 (2/10)² O (1/6)4 O (1/4)6 O (6/10)4 (4/10)2
Question 8. You isolate the DNA from the bacterial cells and apply the Sanger dideoxy sequencing method. You then separate the products of the reactions by gel electrophoresis and obtain the following pattern. What is the sequence of the template and the DNA on the gel? ddATP ddTTP ddCTP ddGTP Template V [ Choose 3'-CTAGTCAAGG-5' 5'-CTAGTCAAGG-3' Sequence 3'-GATCAGTTCC-5' 5'-GATCAGTTCC-3' 5'-GGAACTGATC-3'
Question 1: Look at the following normal and mutant DNA sequences. Normal Sequence (5'-3'): ATG AAC GTT ATC GCA Mutated Sequence (5'-3'): ATG AAT GTC ATC GCA a) What type of mutation has occurred (be specific)? b) Fill in the table for the normal and mutated sequences. Starting with the given 5'-3' sequence, input the complementary DNA, transcribed RNA from the 3'-5' DNA and translated polypeptide sequence for both. Hint: use the codon table! Normal sequence Mutant sequence DNA 5'-3' (given) DNA 3'-5' RNA Polypeptide (use 3 letter codes) c) Based on parts A and B above, what is the ultimate effect this mutation has had on the polypeptide? (1 sentence summary)
Question:- 1. ATT GAC CAA ATC CAT TGA GAC CAA What chains occur when modified DDTP thymine is added to the DNA sequence above.
Agarose gel clectrophoresis is a technique used to separate DNA fragments on an agarose matrix. The DNA is then viewed by staining or under UV light.1068 (i) What is the direction of DNA migration in electric field on the agarose gel? (ii) Explain the principle for the migration of DNA molecules in an electric field. (iii) DNA loading dye is used to prepare DNA markers and samples for loading on the agarose gel. State the function of bromophenol blue in the loading dye.
Question 47 The supercoiled DNA can either be positively or negatively supercoiled. A) True B) False
Question 33 What will be the newly synthesized DNA from the template given? DNA Template 3 - CGGATGCCCGTATAC -5 O 3- GCCTACGGGCATATG -5 O 5- GCCTACGGGCATAAG -3 O 5- GCCTACGGGCATATG -3 O 3- CGGATGCCCGTATAC -5
Show the expected labeled cleavage products if the following DNA segment were subjected to each of the four cleavage reactions: 32P-A-A-C-A-T-G-G-C-G-C-T-T-A-T-G-A-C-G-A
Question 4. What is the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3'? a) Assuming that the four bases are equally likely to be found in a DNA molecule. O (1/4)6 O (1/4)4 O (1/6)4 O 1 in 6 O (1/6)6

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