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A technician prepares a buffer solution that will be used to facilitate the optimal pH for an enzyme involved in the biotechnological degradation of organic compounds. The buffer compound will facilitate a stable pH. To prepare the buffer they need to determine the required concentration of anita hann [conjugate acid or base]_08 They have been provided with the buffer parameters provided in the images. Provide the answer to three decimal places and include an appropriate unit.

A technician prepares a buffer solution that will be used to facilitate the optimal pH for an enzyme involved in the biotechnological degradation of organic compounds. The buffer compound will facilitate a stable pH. To prepare the buffer they need to determine the required concentration of anita hann [conjugate acid or base]_08 They have been provided with the buffer parameters provided in the images. Provide the answer to three decimal places and include an appropriate unit.

Chemistry: Principles and Practice
Chemistry: Principles and Practice
3rd Edition
ISBN:9780534420123
Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Chapter15: Solutions Of Acids And Bases
Section: Chapter Questions
Problem 15.130QE: A solution is made by dissolving 15.0 g sodium hydroxide in approximately 450 mL water. The solution...
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A technician prepares a buffer solution that will be used to facilitate the optimal pH for an enzyme involved in the biotechnological degradation of organic compounds. The buffer compound will facilitate a stable pH. To prepare the buffer they need to determine the required concentration of anita hara [conjugate acid or base]_08 They have been provided with the buffer parameters provided in the images. Provide the answer to three decimal places and include an appropriate unit. Note: You may need to round the numbers to get the required answer. Final volume of solution: 200 ml VEENER Total buffer compound concentration: 150 mM Ratio conjugate base/weak acid: 1.58/1 2000 Concentration weak acid: 0.058 M Final pH of solution: 6.5 Buffer compound pk.: 6.3 14 13 12 11 10 9
Transcribed Image Text:A technician prepares a buffer solution that will be used to facilitate the optimal pH for an enzyme involved in the biotechnological degradation of organic compounds. The buffer compound will facilitate a stable pH. To prepare the buffer they need to determine the required concentration of anita hara [conjugate acid or base]_08 They have been provided with the buffer parameters provided in the images. Provide the answer to three decimal places and include an appropriate unit. Note: You may need to round the numbers to get the required answer. Final volume of solution: 200 ml VEENER Total buffer compound concentration: 150 mM Ratio conjugate base/weak acid: 1.58/1 2000 Concentration weak acid: 0.058 M Final pH of solution: 6.5 Buffer compound pk.: 6.3 14 13 12 11 10 9
A professor prepares a buffer solution that they need for the purification of protein from human cell lysates. They did mix weak acid and conjugate base and obtained an INITIAL solution, which is characterized by the parameters shown in the images. Unfortunately, they did not pay attention to the instructions and added an additional 5 ml of 1M HCl to the initial solution. They were then wondering whether they can still use the solution for their purification experiment and what the pH of the resulting FINAL buffer solution would be? Determine the LINAL H and provide it in the answer how to one decimal place. Chang pH addition of strong base or acid_004 ed answer. Note: You may Hydrochloric Acid M Solution IS17013 500 ml 1 M HCI Final volume of solution: 200 ml Total buffer compound concentration: 150 mM ASSCO Initial concentration weak acid: 0.036 M Initial concentration conjugate base: 0.114 M INITIAL pH of solution: 3.1 Buffer compound pk₂: 2.6 14 13 12 11 10 9
Transcribed Image Text:A professor prepares a buffer solution that they need for the purification of protein from human cell lysates. They did mix weak acid and conjugate base and obtained an INITIAL solution, which is characterized by the parameters shown in the images. Unfortunately, they did not pay attention to the instructions and added an additional 5 ml of 1M HCl to the initial solution. They were then wondering whether they can still use the solution for their purification experiment and what the pH of the resulting FINAL buffer solution would be? Determine the LINAL H and provide it in the answer how to one decimal place. Chang pH addition of strong base or acid_004 ed answer. Note: You may Hydrochloric Acid M Solution IS17013 500 ml 1 M HCI Final volume of solution: 200 ml Total buffer compound concentration: 150 mM ASSCO Initial concentration weak acid: 0.036 M Initial concentration conjugate base: 0.114 M INITIAL pH of solution: 3.1 Buffer compound pk₂: 2.6 14 13 12 11 10 9
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